ObjectivesTo explore the poor population’s cognition and satisfaction on medical assistance policies in Sichuan province, so as to provide evidence for improving health poverty alleviation policies.Methods A telephone survey was conducted between October and December 2017 among 1 280 poor individuals in Sichuan Province, with multi-stage stratified random sampling. The contents of the survey included general demographics of the poor population, and knowledge and satisfaction of health poverty alleviation policies.ResultsThe awareness rate of medical assistance policy was 91.80%, and the satisfaction rate was 91.88%. Poor individuals from non-poor counties, who had been out of poverty, and who reported that they had not signed up for family doctors, had low awareness of poverty alleviation policies. Poor individuals from non-poor counties, who usually went to the municipal hospital, who reported that they have not signed up for family doctors, and who do not know about health policies for poverty alleviation had a lower satisfaction rate.ConclusionsThe overall awareness rate and satisfaction rate of medical assistance policies in Sichuan province are relatively high, however, there are still some shortage. In the future, more attention should be paid to strengthen the promotion of health poverty alleviation policies for non-poor areas and those who had been lifted out of poverty, speeding up the contract service of family doctors and exploring ways to further alleviate the burden of medical expense of patients with serious diseases.
Objective To investigate the influence of different pressures and duration of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expressions of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cell lines including MKN-45, SGC-7901, and MKN-28 were exposed to CO2 in different environments: 0 mm Hg （1 mm Hg=0.133 kPa）, 9 mm Hg （2 h, 4 h）, and 15 mm Hg （2 h, 4 h）. The expressions of mRNA of E-cadherin, intercellular adhesion molecule-1 (ICAM-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor-A (VEGF-A) in the different environments were measured by RT-PCR. The expressions of protein of E-cadherin and ICAM-1 in the environments of 0 mm Hg and 15 mm Hg （4 h） were measured by FCM. Results With the increase of duration or pressure, RT-PCR showed that there was a downward trend in the expression of E-cadherin mRNA as well as there were upward trends in the expressions of ICAM-1, MMP-2, and VEGF-A mRNA; FCM showed that there was a downward trend in the expression of E-cadherin protein while the expression of ICAM-1 protein showed the opposite change. But there were no obvious differences under different environment (P＞0.05). Conclusions Under low pressure (≤15 mm Hg) and short time (≤4 h) of CO2 pneumoperitoneum, the adhesive and invasive ability of gastric cancer cells could not be affected, which means that under this environment, CO2 pneumoperitoneum will not increase the possibility of neoplasm metastasis.
ObjectiveTo compare the biological features of early and late endothelial progenitor cells (EPCs) by isolating and culturing early and late EPCs from the human peripheral blood so as to find some unique properties of EPCs and to propose a suitable strategy for EPCs identification. MethodsMononuclear cells were isolated from the human peripheral blood using density gradient centrifugation. Then, the cells were inoculated in human fibronectin-coated culture flasks and cultured in endothelial cell basal medium 2. After 4-7 days and 2-3 weeks culture, early and late EPCs were obtained respectively. The morphology, proliferation potential, surface markers, cytokine secretion, angiogenic ability, and nitric oxide (NO) release were compared between 2 types of EPCs. Meanwhile, the human aortic endothelial cells (HAECs) were used as positive control. ResultsThe morphology of early and late EPCs was different:early EPCs formed a cell cluster with a spindle shape after 4-7 days of culture, and late EPCs showed a cobblestone appearance. Late EPCs were characterized by high proliferation potential and were able to form capillary tubes on Matrigel, but early EPCs did not have this feature. Both types EPCs could ingest acetylated low density lipoprotein and combine with ulex europaeus Ⅰ. Flow cytometry analysis showed that early EPCs did not express CD34 and CD133, but expressed the CD14 and CD45 of the hematopoietic stem cell markers;however, late EPCs expressed CD31 and CD34 of the endothelial cell markers, but did not express CD14, CD45, and CD133. By RT-PCR analysis, the expressions of vascular endothelial growth receptor 2 and vascular endothelial cadherin in early EPCs were significantly lower than those in the late EPCs and HAECs (P<0.05), but no significant difference was found in the expression of von Willebrand factor and endothelial nitric oxide synthase (eNOS) between 2 type EPCs (P>0.05). The concentrations of vascular endothelial growth factor, granulocyte colony-stimulating factor, and interleukin 8 were significantly higher in the supernatant of early EPCs than late EPCs (P<0.05). Western blot assay indicated eNOS expressed in both types EPCs, while the expression of eNOS in late EPCs was significantly higher than early EPCs at 5 weeks (P<0.05). Both cell types could produce similar amount of NO (P>0.05). ConclusionThe expression of eNOS and the production of NO could be used as common biological features to identify EPCs, and the strategy of a combination of multiple methods for EPCs identification is more feasible.
【摘要】 目的 通過對老年人燒傷的原因進行調查與分析，為預防老年人燒傷提供有效的依據。 方法 調查分析2000年1月-2009年6月收治的270例60歲以上老年燒傷住院患者的致傷因子、好發年齡、燒傷月份、燒傷程度、燒傷地點、家庭及居住情況。 結果 270例老年燒傷中，致傷因子以火焰燒傷最多占147例（54.44%），與其他的致傷因子比較有統計學意義（Plt;0.05）；好發年齡以60～69歲年齡段發生率最高占153例（56.67%），與其他年齡段比較有統計學意義（Plt;0.05）；老年燒傷一年四季均可發生，但以寒冷的冬季12月-次年2月居多，占158例（58.52%），與其他季節比較有統計學意義（Plt;0.05）；燒傷程度以輕度為主共142例（52.59%）；燒傷地點大多發生在家中共213例（78.89%），且為獨居占191例（70.74%）；老年燒傷家庭以農村家庭居多共208例（77.04%）。 結論 隨著老年人群逐步增加，燒傷發生率也增高，在日常生活中應采取有效防范措施，防止和減少老年燒傷的發生。【Abstract】Objective To provide the effective evidence for preventing burn injury of aged people by investigating and analyzing the reasons of burn injury. Methods The injury factors, age, month, degree, place, family and habitation of 270 aged people over 60 years old were analyzed between January, 2000 to June, 2009 in this department. Results In 270 aged patients, there were 147 patients whose injury factors were flame (54.44%) , and there was statistical difference compared with other injury factors (Plt;0.05) . The injury age of the highest incidence rate was 60 to 69 (153 patients, 56.67%) , and there was statistical difference compared with other injury age (Plt;0.05) .The aged burn injury may happen in all seasons, but the highest incidence rate appeared in winter (from December to next February) and there was statistical difference compared with other seasons (Plt;0.05) (158, 58.52%). There were 142 minor injury degree patients mainly (52.59%). The most injury places were at home (213 patients, 78.89%) and 191 patients (70.74%) were living alone.Two hundred and eight patients (77.04%) came from countryside. Conclusion With the aged people increasing, the incidence rate of burn injury is also raised.So effective methods should be adopted to prevent and decrease the incidence of age burn injury.