目的 探討胃嗜酸性肉芽腫的診斷、誤診原因和治療方法。方法 對14例胃嗜酸性肉芽腫的臨床資料進行回顧性分析。結果 全部病例均有上腹疼痛和返酸史； 伴潰瘍形成11例，穿孔4例，上消化道出血3例； 術前行胃鏡檢查2例，X線鋇餐透視檢查6例，無1例獲確診； 其余病例亦全部誤診為胃潰瘍或癌腫。結論 胃鏡多部位取材，特別是在潰瘍與周邊粘膜移行處，采取挖掘式取材，能減少誤診率； 胃大部切除術是主要的治療方法。
目的 探討HIF-1α和BAK蛋白在胃癌中的表達情況，以及二者在胃癌中的相互關系及作用。方法 應用免疫組化SABC染色法檢測80例胃癌組織和20例正常胃組織中的HIF-1α和BAK蛋白的表達情況。結果 胃癌中HIF-lα和BAK蛋白的表達陽性率分別為56.3%（45/80）和67.5%（54/80），而在胃正常組織中分別為5.0%（1/20）和20.0%（4/20），二者在胃癌中的表達顯著高于胃正常組織，其差異有統計學意義（P＜0.05）。HIF-1α蛋白表達與胃癌組織的浸潤范圍、分化程度及淋巴結轉移有關（P＜0.05），與臨床分期、年齡及性別無關（P＞0.05）；BAK蛋白表達與胃癌浸潤及分化程度相關（P＜0.05），與淋巴結轉移、臨床分期、年齡及性別無關（P＞0.05）。胃癌組織中HIF-1α與BAK蛋白的陽性表達之間呈正相關（列聯系數r=0.056，P＜0.05）。結論 HIF-1α與BAK蛋白在胃癌的臨床分期及浸潤轉移中存在關系，這對于研究胃癌的發生和發展，以及對于探索以二者為靶點的抗腫瘤治療有重要意義。
目的 系統評價華蟾素治療胃癌的療效。 方法 計算機檢索Cochrane （2011年第3期），PubMed （1966年1月－2011年9月），EMBASE（1974年1月－2011年9月），中國生物醫學數據庫（CBM，1978年1月－2011年9月），維普（1989年1月－2011年9月），中國期刊全文數據庫（1994年1月－2011年9月）；手工檢索相關雜志。納入華蟾素治療胃癌的隨機對照試驗（RCT），對納入的RCT進行質量評價，并用RevMan 5.0軟件進行Meta分析。 結果 共納入9個RCT，Meta分析結果顯示：華蟾素聯合常規化學療法（化療）治療中晚期胃癌的有效率高于常規化療組，且差異有統計學意義[RR=1.23，95%CI（1.03，1.46），P=0.02]，而1年生存率兩組比較差異無統計學意義[RR=1.28，95%CI（0.69，2.36），P=0.43]。 結論 常規化療加華蟾素治療胃癌的療效優于常規化療，但因為納入研究質量較低，尚需高質量、大樣本、長期的隨機對照試驗進一步驗證上述結論。
Objective To investigate the expression of urokinase-type plasminogen activator (uPA) mRNA in gastric cancer tissues and cancer-adjacent tissues and the relationship between its expression and biologic behavior of tumor. Methods Fourty-eight cases with gastric cancer were detected for the expression of uPA mRNA by fluorogenic probe quantitative reverse transcription polymerase chain reaction (RTPCR). Results The positive expression rate of uPA mRNA was 83.3%, 25.0%, 93.8% and 62.5% in gastric cancer tissues,cancer-adjacent tissues, gastric cancer tissues with lymph node metastasis and with non-lymph node metastasis respectively. Expression of uPA mRNA was positively related with the invasion depth of gastric cancer. Conclusion Expression of uPA mRNA is significantly increased in gastric cancer and it can be used as an indicator to judge the metastasis and prognosis of tumor.
【Abstract】ObjectiveTo study the effect of preoperative gastric arterial chemoembolization on apoptosis of lymph node metastasis of gastric cancer. MethodsForty patients with gastric cancer and lymph node metastasis underwent curative resection, among which there were 20 patients who received the preoperative gastric arterial chemoembolization, and they constituted the treatment group. The rest of the patients were included in the control group. The expressions of p53, CD95 and bcl-2 were examined by immunohistochemistry and apoptosis in the lymph node metastasis was examined by in situ terminal transferasemediated dUTP nick end labeling （TUNEL）. ResultsThe expression intensity of p53 and CD95 in lymph node metastasis of treatment group increased more significantly than that of control group, whereas the expression intensity of bcl-2 decreased in treatment group. There was a significantly positive correlation between the expressions of p53 and CD95 and the apoptosis.ConclusionPreoperative gastric arterial chemoembolization may affect the expressions of p53, CD95 and bcl-2 and may induce the apoptosis of lymph node metastasis. It may be helpful to improve the effect of curative resection of gastric cancer.
【摘要】 目的 觀察糖尿病合并胃癌圍手術期患者應用胰島素泵的治療效果。 方法 2004年1月-2010年12月收治的胃癌合并2型糖尿病患者86例，將患者隨機分為常規組（A組）46例和胰島素泵組（B組）40例，比較兩組患者在血糖控制、手術并發癥方面的差異。 結果 B組與A組出院時，空腹血糖以及餐后血糖均能達標，但B組明顯好于A組（Plt;0.05）；B組胃排空障礙發生率降低（Plt;0.05）。 結論 胰島素泵能更好地控制血糖、減少手術并發癥的發生。【Abstract】 Objective To observe the therapeutic effect of insulin pump on type 2 diabetes mellitus combined with gastric cancer during the perioperative period. Methods Between January 2004 and December 2010, 86 patients with type 2 diabetes mellitus combined with gastric cancer were randomly divided into control group (group A, 46 cases) and insulin pump group (group B, 40 cases). The differences in blood glucose level and infection rate of operation incisions were compared between the two groups. Results Both groups reached the aimed glucose level (fasting blood glucose and postprandial blood glucose) at the discharge from the hospital, which in group B were significantly better than those in group A （Plt;0.05）. The obstruction of gastric emptying in group B decreased significantly (Plt;0.05). Conclusion Insulin pump can control the blood glucose and reduce the surgical complications．
目的探討胃癌根治術后引起急性非結石性膽囊炎的原因及機理。方法回顧性分析2002年1月至2004年11月期間術前未發現膽囊疾患行遠端胃癌根治術的43例患者，術后發生急性非結石性膽囊炎的發病情況。結果43例患者中并發急性非結石性膽囊炎12例。結論胃癌根治術后并發急性非結石性膽囊炎與神經、體液、胃腸道動力、細菌感染、血液供應及內源性凝血因子的激活有關，合理的術式及預防措施有一定的預防作用。
Objective To study the effect of knockdown of signal transducer and activator of transcription 3 (STAT3) expression by short hairpin RNA (shRNA) on proliferation, apoptosis and invasion of human gastric cancer cell line MKN-45 in vitro . Methods Specific shRNA plasmids to STAT3 were constructed, and then transfected into MKN-45 cells by lipofectamine methods. Cells were divided into three groups: control group, psiRNA-H1 transfected group as negative group and psiRNA-H1/STAT3 transfected group. Semi-quantitative RT-PCR and Western blotting were used to detect the expression of STAT3 mRNA and protein, respectively. Proliferation and apoptosis of the transfected cells were observed by methyl thiazolyl tetrazolium (MTT) method and flow cytometry (FCM), respectively. The invasion of the transfected MKN-45 cells was measured by Boyden chamber. Results Compared with the negative control cells, semi-quantitative RT-PCR and Western blotting showed that the expressions of STAT3 mRNA and protein were down-regulated in the psiRNA-H1/STAT3 transfected group ( P ＜ 0.05) . The subcloned recombinant plasmid expressing shRNA effectively inhibited MKN-45 cell growth and proliferation while empty plasmid had no such specific effect. Cell apoptosis rate increased significantly in psiRNA-H1/STAT3 transfected group （ P ＜ 0.01）, and the invasion of MKN-45 cells was efficiently inhabited in psiRNA-H1/STAT3 transfected group as compared with control group and psiRNA-H1 transfected group( P ＜ 0.01).Conclusion Recombinant plasmid psiRNA-H1/STAT3 shRNA significantly inhibits the proliferation and invasion of MKN-45 cells and promotes their apoptosis.
ObjectiveTo study the effects and mechanism of Octreotide to inhibit the proliferation of human gastric cancer cells in vitro. MethodsHuman gastric cancer cell line SGC7901 was treated with Octreotide. Human fibroblast cell line HF and 5FU were used as control. MTT assay and fluorescent microscopy as well as flow cytometry were performed in this study. ResultsOctreotide inhibited the growth of SGC7901 in vitro within certain concentrations. The suppression was quantity dependent but did not occur when up to a certain concentration. There was no difference between Octreotide and 5FU in their inhibition on SGC7901. Octreotide had no effects on normal human fibroblast cell line HF. When SGC7901 was treated with Octreotide, the typical apoptotic bodies were identified by flow cytometry and fluorescent microscopy. ConclusionOctreotide can inhibit the proliferation of human gastric cancer cell line SGC7901 in vitro. The induction of apoptosis by Octreotide might be the primary mechanism.
ObjectiveTo evaluate the value of omega-3 fish oil fat emulsion in parenteral nutrition support after radical resection of gastric cancer patients.MethodsThe clinical data of 80 patients in Tongling Peopl’s Hospital undergoing radical resection of gastric cancer from March 2018 to September 2019 were enrolled by retrospective method. Of them, 40 patients were treated with routine parenteral nutrition (control group) and 40 patients were treated with extra 100 mL omega-3 fish oil fat emulsion after operation (observation group). Nutritional and stress indicators included blood total albumin (TP), albumin (ALB), c-reactive protein (CRP), and procalcitonin (PCT) on one day before operation, and 1st, 3rd, and 7th day after operation were collected. The collected indexes of liver function included blood cholesterol (CHO), triglyceride (TG), total bilirubin (TBIL), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma glutamyl transpeptidase (γ-GT) on one day before operation, and 3rd and 7th days after operation.Results① Nutritional indicator. There was no significant differences in TP and ALB levels between the two groups on one day before operation, and on the 1st, 3rd, and 7th day after operation (P>0.05). In the same group compared with one day before operation, the average TP level of the control group and the observation group decreased on the 1st, 3rd, and 7th day after operation (P<0.05), and in the control group and the observation group, the ALB level decreased on the 1st day after operation (P<0.05), and increased on the 7th day after operation (P<0.05). ② Stress index. There was no significant difference in CRP and PCT levels between the two groups on one day before and one day after operation (P>0.05), and the CRP and PCT levels in the observation group were lower than those in the control group on the 3rd day and 7th day after operation (P<0.05). Compared with the CRP and PCT levels on the one day before operation, the average CRP and PCT levels in the observation group and the control group increased on the 1st, 3rd, and 7th day after operation (P<0.05). ③ Liver function index. There was no significant differences in CHO, TG, and γ-GT levels between the two groups on one day before operation and the 3rd day after operation (P>0.05), but the above indexes of observation group were lower on the 7th day after operation than those of the control group (P<0.05). There was no significant differences on TBIL, ALT, and ALP levels between the postoperative observation group and the control group (P>0.05). Compared with one day before operation in the same group, there was no significant difference on CHO and ALP levels on 3rd and 7th day after operation in both the observation group and control group (P>0.05). There was no significant differences in TG and TBIL levels in the observation group on 3rd and 7th day after operation (P>0.05), but the TG and TBIL levels in the control group were increased on 7th after operation (P<0.05), and the ALT and γ-GT levels in the observation group and the control group on 7th day after operation were increased in the same group compared with one day before operation (P<0.05).ConclusionParenteral nutrition with omega-3 fish oil fat emulsion do not improve the recent postoperative nutritional status, but reduce the inflammatory stress response and protecte liver function in patients with gastric cancer after operation.