Objective To investigate the effect s of T lymphoma invasion and metastasis inducing factor 1 ( Tiam 1) antisense oligonucleotides (ASODN) on morphological remodeling of gast ric cancer cells. Methods The high-invasive and metastastic subgroup (MH ) was separated f rom human gast ric cancer cell line MKN245 (M0 ) by laminin adhesion method in vi t ro. And they were divided into four group s according to different further t reatment s : no t ransfection group (cont rol group ) , liposome t ransfection group , sense oligonucleotides2liposome t ransfection group ( SODN t ransfection with liposome group ) and antisense oligonucleotides2liposome t ransfection group (ASODN t ransfection with liposome group) . Then the expressions of Tiam 1 mRNA and protein were detected by RT-PCR and flowcytomet ry , respectively. The morphology changes between Tima 1 ASODN t ransfected MH cells and no t ransfected cells were observed by using HE stain , cytoskeletal protein stain and scanning elect ronic microscope (SEM) . Results Compared with the other group s , the expressions of Tiam 1 mRNA and protein in MH cells were significantly decreased af ter the cells were t ransfected with 0. 43 μmol/ L Tiam 1 ASODN ( P lt; 0. 01) . Additionally , it was observed that the t ransfected MH cells had less membrane surface projections , fewer or shortener pseudopodia , less irregular cytoskeletal network and less spotted-like actin bodys than no t ransfected MH cells did. Conclusion ASODN t ransfection could effectively suppress the expression of Tiam 1 and the remodeling in gast ric cancer cells , which may play an important role in the invasion and metastasis of gast ric cancer cells.
【Abstract】Objective To explore the effect against gastric cancer induced by Newcastle disease virus modified autologous tumor vaccine （NDV-ATV）pulsed dendritic cells(DCs). Methods The Newcastle disease virus infected the gastric cancer lines (MNK45) and was lost its activity. Peripheral blood mononuclear cell (PBMC) were cultured under condition of recombinant human granulocyte macrophage-colony stimulating factor (1 000 u/ml)+IL-4(1 000 u/ml) + TNF-α(100 ng/ml). The tumor antigen specific cytotoxic T lymphocytes (CTL) was generated from activated autologous T cell by the Newcastle disease virus infected the MNK45 pulsed DC. And Cyto Tox 96TM in vitro assayed the cytotoxicity of CTL to MNK45. Thawed gastric cancer cell antigen were used as control in these experiments. Results The killing rate of MNK45 by antigen specific CTL reached (90.15±9.82)%, which was nearly twice as high as that of control(60.57±5.74)%. The CTL had much higher cytotoxicity to different differentiated type of gastric cancer cells such as MGC803〔(52.23±6.45)% 〕 and SGC7901〔 (61.75±8.84)%〕, as compared with LOVO〔(9.11±3.42)%〕 and HepG2 〔 (8.30±3.12)%〕tumor cells(P＜0.05). Conclusion Efficient and specific of against gastric cancer immunoreaction can be induced in virtue of NDV-ATV pulsed DCs, NDV-ATV loaded DCs might provide a new kind of theraputic means for gastric cancer.
目的 探討腹腔鏡下乙狀結腸癌根治術保留盆腔自主神經的可行性及對術后泌尿、生殖功能的影響。 方法 選取2007年8月－2009年7月60例行腹腔鏡下乙狀結腸癌根治術患者，手術按標準腹腔鏡下乙狀結腸癌前切除手術方式操作。術后采用問卷調查方式對患者術后泌尿功能、男性勃起功能、女性性功能進行效果評價。生殖泌尿功能評估包含國際前列腺增生評分（IPSS）、國際勃起功能評分量表（IIEF）、女性性功能評估量表（FSFI）。 結果 58例患者成功保留自主神經，56例接受術后泌尿、生殖功能測定，置放尿管時間為1～7 d，平均3 d，拔除尿管后排尿功能良好（IPSS 0～7分）57例（95.0%），一般（IPSS 8～14分）2例（3.3%），差（IPSS 15～35分）1例（1.7%），術前、術后IPSS評分差異無統計學意義（P=0.075）。30例男性患者術后，射精良好27例（90.0%），一般2例（6.7%），差1例（3.3%）；但是勃起功能測定良好（IIEF 60～75分）28例（93.4%），一般（IIEF 44～59分）1例（3.3%），差（IIEF 5～43分）1例（3.3%），術前、術后IIEF差異無統計學意義。26例女性患者術后性功能評分，良好（FSFI 76～95分） 23例（88.5%），一般（FSFI 58～75分） 2例（7.7%），差（FSFI 4～57分）1例（3.8%）。盆腔神經保留成功的患者術前、術后差異無統計學意義（P=0.122）。 結論 多數腹腔鏡下乙狀結腸癌根治術患者能有效保留盆腔自主神經，且術后能保留泌尿、生殖功能。
ObjectiveTo investigate the short-term outcomes and the impact on postoperative peritoneal implantation after laparoscopic radical gastrectomy with natural orifice specimen extraction surgery (NOSES) compared with small incision specimen extraction. MethodsPatients who underwent standard laparoscopic radical gastrectomy with NOSES (NOSES group) admitted to The Second People’s Hospital of Yibin from September 2022 to October 2024 were retrospectively enrolled. Propensity score matching (PSM) at a 1∶1 ratio was performed to select patients receiving standard laparoscopic radical gastrectomy with small incision specimen extraction as controls (small incision group). Operative indicators, visual analogue scale (VAS) points at different postoperative time points (6 h, 12 h, 24 h, 72 h), oncological outcomes, complications, and patients’ satisfaction were compared between the two groups. ResultsA total of 252 patients were enrolled. After PSM, 102 patients were included in the NOSES group and the small incision group respectively, and the baseline characteristics were well balanced between the two groups (all standardized mean differences <0.2). There were no statistically significant differences in operation time and intraoperative blood loss between the NOSES group and the small incision group (P>0.05). The time to first postoperative flatus, first ambulation, first liquid diet intake, and postoperative hospital stay were significantly shorter in the NOSES group than those in the small incision group (P<0.05). Repeated-measures analysis of variance for postoperative VAS pain points at multiple time points revealed a significant group-by-time interaction effect (Finteraction=58.969, P<0.001). VAS pain points gradually decreased over postoperative time in both groups (Ftime=102.582, P<0.001), and the main effect of group was statistically significant (Fgroup=8.635, P<0.001). At all identical observation time points of 6 h, 12 h, 24 h and 72 h postoperatively, VAS pain points were consistently lower in the NOSES group than those in the small incision group (all P<0.05). No statistically significant differences were observed between the NOSES group and the small incision group (all P>0.05) in the number of harvested lymph nodes [(35.67±9.78) per case vs. (36.12±10.23) per case], proximal resection margin length [(4.23±1.56) cm vs. (4.05±1.78) cm], distal resection margin length [(6.89±2.34) cm vs. (7.11±2.45) cm], R0 resection rate (100% vs. 100%), local recurrence rate [1.96% (2/102) vs. 0.98% (1/102)], peritoneal implantation rate [1.96% (2/102) vs. 0.98% (1/102)], distant metastasis rate [0.98% (1/102) vs. 0.98% (1/102)], and overall complication rate [2.94% (3/102) vs. 2.94% (3/102)]. The overall satisfaction rate was significantly higher in the NOSES group as compared with the small incision group [98.04% (100/102) vs. 80.39% (82/102), P<0.001]. ConclusionsThe results of this study suggest that both NOSES and small incision specimen retrieval after laparoscopic radical gastrectomy can achieve favorable therapeutic efficacy and safety. In addition, NOSES specimen extraction can alleviate postoperative pain, accelerate the recovery process, and improve patients’ satisfaction.
ObjectiveConstructing a prediction model for seizures after stroke, and exploring the risk factors that lead to seizures after stroke. MethodsA retrospective analysis was conducted on 1 741 patients with stroke admitted to People's Hospital of Zhongjiang from July 2020 to September 2022 who met the inclusion and exclusion criteria. These patients were followed up for one year after the occurrence of stroke to observe whether they experienced seizures. Patient data such as gender, age, diagnosis, National Institute of Health Stroke Scale (NIHSS) score, Activity of daily living (ADL) score, laboratory tests, and imaging examination data were recorded. Taking the occurrence of seizures as the outcome, an analysis was conducted on the above data. The Least absolute shrinkage and selection operator (LASSO) regression analysis was used to screen predictive variables, and multivariate Logistic regression analysis was performed. Subsequently, the data were randomly divided into a training set and a validation set in a 7:3 ratio. Construct prediction model, calculate the C-index, draw nomogram, calibration plot, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA) to evaluate the model's performance and clinical application value. ResultsThrough LASSO regression, nine non-zero coefficient predictive variables were identified: NIHSS score, homocysteine (Hcy), aspartate aminotransferase (AST), platelet count, hyperuricemia, hyponatremia, frontal lobe lesions, temporal lobe lesions, and pons lesions. Multivariate logistic regression analysis revealed that NIHSS score, Hcy, hyperuricemia, hyponatremia, and pons lesions were positively correlated with seizures after stroke, while AST and platelet count were negatively correlated with seizures after stroke. A nomogram for predicting seizures after stroke was established. The C-index of the training set and validation set were 0.854 [95%CI (0.841, 0.947)] and 0.838 [95%CI (0.800, 0.988)], respectively. The areas under the ROC curves were 0.842 [95%CI (0.777, 0.899)] and 0.829 [95%CI (0.694, 0.936)] respectively. Conclusion These nine variables can be used to predict seizures after stroke, and they provide new insights into its risk factors.