目的研究外源性胰島素對糖尿病大鼠缺血后肢血管內皮生長因子(VEGF)表達的影響及其促血管新生的作用。方法取20只健康雄性SD大鼠,將其右后肢股動、靜脈及其分支和屬支結扎，制成糖尿病大鼠后肢缺血模型，然后將其用簡單隨機化方法隨機平均分為模型組與治療組，另取10只正常大鼠作為對照組。14 d后處死大鼠，應用 Western blot法檢測大鼠后肢肌肉組織中VEGF蛋白表達水平，并采用堿性磷酸酶(AKP)染色法測定大鼠后肢肌肉組織中毛細血管密度。結果對照組大鼠術前和術后7 d體重和血糖水平比較差異無統計學意義(Pgt;0.05); 模型組大鼠術后7 d與術前比較，體重明顯下降(Plt;0.05)，但血糖水平差異無統計學意義(Pgt;0.05); 而治療組給予皮下注射胰島素注射液術后7 d較術前體重明顯下降，并且血糖水平較術前也明顯下降，差異均有統計學意義(Plt;0.05)。與對照組比較，治療組及模型組大鼠術后7 d體重明顯下降、血糖明顯升高(Plt;0.05，Plt;0.01); 治療組與模型組比較，體重差異無統計學意義(Pgt;0.05)，但治療組大鼠術后7 d血糖水平較模型組明顯降低(Plt;0.05)。治療組大鼠缺血肢體肌肉組織中VEGF蛋白相對表達量(155.06±10.26)明顯高于模型組(94.30±11.23)，Plt;0.05; 對照組大鼠未檢測到VEGF蛋白表達。對照組大鼠右后肢肌肉組織中毛細血管密度明顯高于模型組和治療組(Plt;0.05)，而治療組又明顯高于模型組(Plt;0.05)。3組大鼠左后肢毛細血管密度差異無統計學意義(Pgt;0.05); 對照組大鼠左、右后肢毛細血管密度差異無統計學意義(Pgt;0.05); 模型組和治療組大鼠右后肢毛細血管密度均明顯低于左后肢(Plt;0.05)。結論胰島素可以增強糖尿病大鼠后肢缺血肌肉組織中VEGF蛋白表達，促進毛細血管生成，發揮保護作用。
本文研究殼聚糖-左氧氟沙星長效緩釋敷料的皮膚刺激性和燒傷治療效果。通過參照GB/T16886.10-2005評價該敷料的皮膚刺激性,同時制備燒傷兔模型,分為三組,空白對照組用凡士林油紗包扎,陽性對照組用納米銀創傷貼包扎,實驗樣品組用殼聚糖-左氧氟沙星長效緩釋敷料包扎,分別于3、6、9、14、21、28 d進行傷口未愈合面積測量、羥脯氨酸含量及組織病理學檢查。實驗結果顯示該敷料原發性刺激指數為0;實驗樣品組和陽性對照組在6、9、14、21 d時,未愈合傷口面積均小于空白對照組(P＜0.05),實驗樣品組在9 d和14 d時其未愈合傷口面積顯著低于陽性對照組(P＜0.05);實驗樣品組在6、9和14 d時羥脯氨酸含量顯著高于陽性對照組和空白對照組(P＜0.05);通過病理學觀察,在21 d時實驗樣品組最早出現附屬器。由此說明殼聚糖-左氧氟沙星長效緩釋敷料的皮膚刺激性極輕微,能夠有效促進燒傷創面愈合。
【摘要】 目的 研究2型糖尿病（type 2 diabetes mellitus，T2DM）合并非酒精性脂肪性肝病（non-alcoholic fatty liver disease， NAFLD）患者血漿脂肪細胞特異性脂肪酸結合蛋白（adipocyte-specific fatty acid-binding protein，A-FABP）的水平及其相關因素。 方法 2009年10月—2010年10月選取T2DM合并NAFLD組（A組）60例，未合并NAFLD組56例（B組）為研究對象。測定體質量指數（body mass index，BMI），檢測血脂、糖化血紅蛋白（hemoglobin A1c，HbA1c）等生化指標。放射免疫法測定空腹胰島素（fasting insulin，FINS），空腹C肽水平（fasting C-peptide，FCP），計算胰島素抵抗指數（homeostasis model of assessment-insulin resistance，HOMA-IR）、胰島素敏感指數（insulin sensitivity index，ISI），測定A-FABP、C反應蛋白（C-reaction protein，CRP）及腫瘤壞死因子-α（tumor necrosis factor-α，TNF-α）。 結果 與B組患者相比，A組患者其血漿A-FABP水平、BMI、腰圍、腰臀比、丙氨酸氨基轉移酶、門冬氨酸氨基轉移酶、CRP、TNF-α、FCP、FINS、總膽固醇、甘油三酯、Ln（HOMA-IR）升高，Ln（ISI）降低，差異有統計學意義（Plt;0.05）；兩組HbA1c差異無統計學意義（Pgt;0.05）。A-FABP水平變化與TNF-α、HOMA-IR、CRP呈正相關，與ISI呈負相關。 結論 T2DM伴NAFLD中，A-FABP升高與胰島素抵抗是并存的，且存在明顯相關關系，二者在疾病的發生發展中均可能具有重要的作用。【Abstract】 Objective To analyze the serum level of adipocyte-specific fatty acid-binding protein (A-FABP) in patients with type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD), and its related factors. Methods From October 2009 to October 2010, 112 patients with T2DM were categorized into two groups: the group with NAFLD (group A) with 60 patients, and the group without NAFLD (group B) with 56 patients. Body mass index (BMI), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and hemoglobin A1c (HbA1c) were detected. Radioimmunoassay was carried out to measure fasting insulin (FINS) and fasting C-peptide (FCP), and homeostasis model of assessment-insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated. At the same time, A-FABP, C-reaction protein (CRP) and tumor necrosis factor-α (TNF-α) were also detected. Results Compared with patients in group B, plasma levels of A-FABP, BMI, waistline, waist to hip ratio, ALT, AST, TG, TC, HDL-C, LDL-C, CRP, FCP, FINS, and HOMA-IR for patients in group A were all higher, while ISI was lower; and the differences in the above-mentioned parameters were statistically significant (Plt;0.05). The levels of HbA1c in the two groups were not significantly different (Pgt;0.05). The change of A-FABP level was positively correlated with TNF-α, HOMA-IR and CRP, while it wasnegatively correlated with ISI. Conclusions In patients with T2DM with NAFLD, there is an obvious correlation between the coexisting A-FABP rise and insulin resistance. Both of them played critical roles in the onset and developing of the disease.