Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WANG Yanqiu ¹ , XU Lili ² , JIANG Tao ¹ , XUE Juan ¹

1. Department of Laboratory, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P. R. China;
2. Institute of Genetics and Reproductive Medicine, Nantong Maternal and Child Health Hospital, Nantong, Jiangsu 226000, P. R. China;

Corresponding?author：

WANG Yanqiu, Email: wjm7410z@163.com

Keywords：

ulcerative colitis; long non-coding RNA ANRIL; procalcitonin; diagnostic value

DOI：

10.7507/1007-9424.202103100

Video：

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Objective To investigate the level of serum long non-coding RNA antisense non-coding RNA INK4 locus (LncRNA ANRIL) in patients with ulcerative colitis (UC), and to analyze the diagnostic value of serum LncRNA ANRIL level in UC. Methods A total of 143 UC patients admitted to the First Affiliated Hospital of Henan University of Science and Technology from February 2015 to November 2019 were retrospectively analyzed, and 145 healthy people with normal physical examination in the First Affiliated Hospital of Henan University of Science and Technology were selected as the control group. The relationship between serum LncRNA ANRIL level and PCT/IL-17 level was analyzed, the serum levels of LncRNA ANRIL, PCT, and IL-17 were compared between the two groups, and their diagnostic value for UC was explored.Results The disease degree of 143 UC patients: 41 cases were mild, 59 cases were moderate, and 43 cases were severe; endoscopic grade: 38 cases were grade Ⅰ, 65 cases were grade Ⅱ, and 40 cases were grade Ⅲ. Compared with the control group, the serum levels of LncRNA ANRIL, PCT, and IL-17 were increased in the UC group (P<0.05); the levels of serum LncRNA ANRIL, PCT, and IL-17 in the UC group increased gradually with the increase of disease severity and endoscopic grade (P<0.05). The serum levels of LncRNA ANRIL were positively correlated with the levels of PCT and IL-17 in the UC patients (r=0.596, P<0.001; r=0.492, P<0.001). The area under the curve (AUC) of serum LncRNA ANRIL level in the diagnosis of UC was 0.851, the cut-off value was 1.29, the sensitivity and specificity were 75.5% and 83.4%, respectively. The AUC of serum LncRNA ANRIL combined with PCT in the diagnosis of UC was 0.898, the corresponding sensitivity and specificity were 81.8% and 87.6%, respectively. The sensitivity and diagnostic value of combination of LncRNA ANRIL and PCT were higher than that of serum LncRNA ANRIL alone (Z=2.102, P=0.036). Conclusions The serum level of LncRNA ANRIL in UC patients is increased, which has a certain diagnostic value, and it combines with PCT can better predict UC.

Citation： WANG Yanqiu, XU Lili, JIANG Tao, XUE Juan. Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2021, 28(11): 1439-1444. doi: 10.7507/1007-9424.202103100 Copy

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2.	蔣麗琳, 肖南平, 楊興平, 等. CTGF mRNA在潰瘍性結腸炎組織中的表達及與病情和預后的關系. 國際消化病雜志, 2020, 40(2): 125-129.
3.	Mohammed Vashist N, Samaan M, Mosli MH, et al. Endoscopic scoring indices for evaluation of disease activity in ulcerative colitis. Cochrane Database Syst Rev, 2018, 1(1): 11450-11456.
4.	劉麗, 柳云恩. 潰瘍性結腸炎患者血清miR-15表達水平與炎性反應狀態及預后的關系. 國際消化病雜志, 2020, 40(1): 41-46.
5.	Yarani R, Mirza AH, Kaur S, et al. The emerging role of lncRNAs in inflammatory bowel disease. Exp Mol Med, 2018, 50(12): 1-14.
6.	Qiao C, Yang L, Wan J, et al. Long noncoding RNA ANRIL contributes to the development of ulcerative colitis by miR-323b-5p/TLR4/MyD88/NF-κB pathway. Biochem Biophys Res Commun, 2019, 508(1): 217-224.
7.	中華中醫藥學會. 潰瘍性結腸炎診療指南. 中國中醫藥現代遠程教育, 2011, 9(10): 126-128.
8.	Jain S, Kedia S, Bopanna S, et al. Are Truelove and Witts criteria for diagnosing acute severe colitis relevant for the Indian population? A prospective study. Intest Res, 2018, 16(1): 69-74.
9.	Landy J, Wahed M, Peake ST, et al. Oral tacrolimus as maintenance therapy for refractory ulcerative colitis–an analysis of outcomes in two London tertiary centres. J Crohns Colitis, 2013, 7(11): e516-e521.
10.	Berends SE, Strik AS, L?wenberg M, et al. Clinical pharmacokinetic and pharmacodynamic considerations in the treatment of ulcerative colitis. Clin Pharmacokinet, 2019, 58(1): 15-37.
11.	李晶瑩, 孫斐, 劉智娟. 受體相互作用蛋白激酶1在潰瘍性結腸炎患者組織中的表達及意義. 中國臨床藥理學雜志, 2020, 36(23): 3888-3891.
12.	Gonzalez DO, Nwomeh BC. Complications in children with ulcerative colitis undergoing ileal pouch-anal anastomosis. Semin Pediatr Surg, 2017, 26(6): 384-390.
13.	高成超, 程永波. 血小板異常與潰瘍性結腸炎關系的研究進展. 山東醫藥, 2020, 60(35): 109-111.
14.	Lu C, Yang M, Luo F, et al. Prediction of lncRNA-disease associations based on inductive matrix completion. Bioinformatics, 2018, 34(19): 3357-3364.
15.	賀林, 程龍, 周程繼, 等. 長鏈非編碼RNA結腸癌相關轉錄因子1與胃癌的研究進展. 中國普外基礎與臨床雜志, 2020, 27(9): 1180-1183.
16.	Zacharopoulou E, Gazouli M, Tzouvala M, et al. The contribution of long non-coding RNAs in inflammatory bowel diseases. Dig Liver Dis, 2017, 49(10): 1067-1072.
17.	孟云超, 牛桂軍. 炎癥性腸病中長鏈非編碼RNA的研究進展. 重慶醫學, 2019, 48(10): 1741-1744.
18.	彭志浩, 李玉明. 長鏈非編碼RNA與結腸癌發生、發展關系的研究進展. 醫學研究雜志, 2020, 49(3): 9-11.
19.	Chen SW, Wang PY, Liu YC, et al. Effect of long noncoding RNA H19 overexpression on intestinal barrier function and its potential role in the pathogenesis of ulcerative colitis. Inflamm Bowel Dis, 2016, 22(11): 2582-2592.
20.	Zhou T, Wu L, Zong Z, et al. Long non-coding RNA mortal obligate RNA transcript inhibits the migration and invasion of colon cancer cells by inactivating transforming growth factor β1. Oncol Lett, 2020, 19(2): 1131-1136.
21.	Sun Y, Zheng ZP, Li H, et al. ANRIL is associated with the survival rate of patients with colorectal cancer, and affects cell migration and invasion in vitro. Mol Med Rep, 2016, 14(2): 1714-1720.
22.	Liu XW, Ma T, Liu W, et al. Sustained increase in angiopoietin-2, heparin-binding protein, and procalcitonin is associated with severe sepsis. J Crit Care, 2018, 45(1): 14-19.
23.	張濤, 朱孝明, 楊俊玲. D-二聚體、PCT和CRP檢測在重癥胰腺炎中的臨床價值. 解放軍預防醫學雜志, 2020, 38(1): 17-18.
24.	Wu HM, Wei J, Li J, et al. Serum procalcitonin as a potential early predictor of short-term outcomes in acute severe ulcerative colitis. Dig Dis Sci, 2019, 64(11): 3263-3273.
25.	Lee MH, Tung-Chieh Chang J, Liao CT, et al. Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients. Oncotarget, 2018, 9(11): 9825-9837.
26.	馬乾章, 李云, 丁原全. 白頭翁湯加味聯合美沙拉嗪緩釋片治療熱毒熾盛型潰瘍性結腸炎的臨床療效觀察. 海南醫學院學報, 2019, 25(12): 918-922.

1. Fischer S, Rath T, Neurath MF. Inflammatory bowel diseases: Crohn’s disease and ulcerative colitis. Internist (Berl), 2018, 59(7): 681-693.
2. 蔣麗琳, 肖南平, 楊興平, 等. CTGF mRNA在潰瘍性結腸炎組織中的表達及與病情和預后的關系. 國際消化病雜志, 2020, 40(2): 125-129.
3. Mohammed Vashist N, Samaan M, Mosli MH, et al. Endoscopic scoring indices for evaluation of disease activity in ulcerative colitis. Cochrane Database Syst Rev, 2018, 1(1): 11450-11456.
4. 劉麗, 柳云恩. 潰瘍性結腸炎患者血清miR-15表達水平與炎性反應狀態及預后的關系. 國際消化病雜志, 2020, 40(1): 41-46.
5. Yarani R, Mirza AH, Kaur S, et al. The emerging role of lncRNAs in inflammatory bowel disease. Exp Mol Med, 2018, 50(12): 1-14.
6. Qiao C, Yang L, Wan J, et al. Long noncoding RNA ANRIL contributes to the development of ulcerative colitis by miR-323b-5p/TLR4/MyD88/NF-κB pathway. Biochem Biophys Res Commun, 2019, 508(1): 217-224.
7. 中華中醫藥學會. 潰瘍性結腸炎診療指南. 中國中醫藥現代遠程教育, 2011, 9(10): 126-128.
8. Jain S, Kedia S, Bopanna S, et al. Are Truelove and Witts criteria for diagnosing acute severe colitis relevant for the Indian population? A prospective study. Intest Res, 2018, 16(1): 69-74.
9. Landy J, Wahed M, Peake ST, et al. Oral tacrolimus as maintenance therapy for refractory ulcerative colitis–an analysis of outcomes in two London tertiary centres. J Crohns Colitis, 2013, 7(11): e516-e521.
10. Berends SE, Strik AS, L?wenberg M, et al. Clinical pharmacokinetic and pharmacodynamic considerations in the treatment of ulcerative colitis. Clin Pharmacokinet, 2019, 58(1): 15-37.
11. 李晶瑩, 孫斐, 劉智娟. 受體相互作用蛋白激酶1在潰瘍性結腸炎患者組織中的表達及意義. 中國臨床藥理學雜志, 2020, 36(23): 3888-3891.
12. Gonzalez DO, Nwomeh BC. Complications in children with ulcerative colitis undergoing ileal pouch-anal anastomosis. Semin Pediatr Surg, 2017, 26(6): 384-390.
13. 高成超, 程永波. 血小板異常與潰瘍性結腸炎關系的研究進展. 山東醫藥, 2020, 60(35): 109-111.
14. Lu C, Yang M, Luo F, et al. Prediction of lncRNA-disease associations based on inductive matrix completion. Bioinformatics, 2018, 34(19): 3357-3364.
15. 賀林, 程龍, 周程繼, 等. 長鏈非編碼RNA結腸癌相關轉錄因子1與胃癌的研究進展. 中國普外基礎與臨床雜志, 2020, 27(9): 1180-1183.
16. Zacharopoulou E, Gazouli M, Tzouvala M, et al. The contribution of long non-coding RNAs in inflammatory bowel diseases. Dig Liver Dis, 2017, 49(10): 1067-1072.
17. 孟云超, 牛桂軍. 炎癥性腸病中長鏈非編碼RNA的研究進展. 重慶醫學, 2019, 48(10): 1741-1744.
18. 彭志浩, 李玉明. 長鏈非編碼RNA與結腸癌發生、發展關系的研究進展. 醫學研究雜志, 2020, 49(3): 9-11.
19. Chen SW, Wang PY, Liu YC, et al. Effect of long noncoding RNA H19 overexpression on intestinal barrier function and its potential role in the pathogenesis of ulcerative colitis. Inflamm Bowel Dis, 2016, 22(11): 2582-2592.
20. Zhou T, Wu L, Zong Z, et al. Long non-coding RNA mortal obligate RNA transcript inhibits the migration and invasion of colon cancer cells by inactivating transforming growth factor β1. Oncol Lett, 2020, 19(2): 1131-1136.
21. Sun Y, Zheng ZP, Li H, et al. ANRIL is associated with the survival rate of patients with colorectal cancer, and affects cell migration and invasion in vitro. Mol Med Rep, 2016, 14(2): 1714-1720.
22. Liu XW, Ma T, Liu W, et al. Sustained increase in angiopoietin-2, heparin-binding protein, and procalcitonin is associated with severe sepsis. J Crit Care, 2018, 45(1): 14-19.
23. 張濤, 朱孝明, 楊俊玲. D-二聚體、PCT和CRP檢測在重癥胰腺炎中的臨床價值. 解放軍預防醫學雜志, 2020, 38(1): 17-18.
24. Wu HM, Wei J, Li J, et al. Serum procalcitonin as a potential early predictor of short-term outcomes in acute severe ulcerative colitis. Dig Dis Sci, 2019, 64(11): 3263-3273.
25. Lee MH, Tung-Chieh Chang J, Liao CT, et al. Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients. Oncotarget, 2018, 9(11): 9825-9837.
26. 馬乾章, 李云, 丁原全. 白頭翁湯加味聯合美沙拉嗪緩釋片治療熱毒熾盛型潰瘍性結腸炎的臨床療效觀察. 海南醫學院學報, 2019, 25(12): 918-922.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis

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