低分子肝素鈣對急性深靜脈血栓大鼠血清IL-6和TNF-α水平的影響_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 李安強 , 史浩 , 牛啟兵 ,  陳泉 , 董方

甘肅省人民醫院血管外科（甘肅蘭州 730000）;

Corresponding?author：

陳泉, Email: 13919093907@163.com

Keywords：

低分子肝素鈣; 深靜脈血栓; 炎性反應

DOI：

10.7507/1007-9424.20160286

Video：

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目的探討低分子肝素鈣（LMWH）對急性下肢深靜脈血栓（DVT）大鼠血清白介素6（IL-6）和腫瘤壞死因子α（TNF-α）水平的影響。方法將57只SD大鼠隨機分為空白對照組20只和DVT組37只，DVT組予以結扎近心端股靜脈，空白對照組大鼠不予結扎。再將DVT組大鼠隨機分為實驗對照組18只和LMWH組19只。空白對照組和實驗對照組大鼠均給予2 mL生理鹽水皮下注射，LMWH組大鼠給予2 mL LMWH皮下注射，每12小時注射1次，60 h后停止注射。分別于術前、術后36 h和術后72 h測量3組大鼠左下肢膝關節上6 mm處的左下肢周徑及血清IL-6和TNF-α水平，并進行組間比較。結果術前空白對照組、實驗對照組及LMWH組的左下肢周徑、血清IL-6水平和血清TNF-α水平比較差異均無統計學意義（P＞0.050），但術后36 h和術后72 h 3組的左下肢周徑、血清IL-6水平和血清TNF-α水平比較差異均有統計學意義（P＜0.050），均是實驗對照組＞LMWH組＞空白對照組。結論急性下肢DVT SD大鼠行LMWH治療后其血清IL-6和TNF-α水平均明顯降低，提示LMWH對急性下肢DVT具有抗炎作用。

Citation： 李安強, 史浩, 牛啟兵, 陳泉, 董方. 低分子肝素鈣對急性深靜脈血栓大鼠血清IL-6和TNF-α水平的影響. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(9): 1121-1124. doi: 10.7507/1007-9424.20160286 Copy

1.	黨曉衛, 姚振濤, 喬師師, 等.兔下腔靜脈球囊損傷后血管重塑相關因子的變化及G-CSF的干預作用.中國普通外科雜志, 2013, 22(6):721-725.
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4.	曹化, 居克舉, 倪桂華, 等.彈力襪預防出血性卒中患者下肢深靜脈血栓形成的有效性及安全性觀察.中國全科醫學, 2011, 14(3):333-335.
5.	Lytvyn OI, Kopitsa MP, Petyunina OV. Interaction between inflammation and thrombosis in acute coronary syndrome. Kardiol Pol, 2004, 61(8):110-116.
6.	Salobir B, Sabovic M. Possible vascular-bed-specific role of interleukin-6 in young women with a history of myocardial infarction, lacunar cerebral infarction and deep vein thrombosis. Cytokine, 2004, 25(6):265-272.
7.	Esmon CT. The impact of the inflammatory response on coagulation. Thromb Res, 2004, 114(5):321-327.
8.	van Aken BE, Reitsma PH, Rosendaal FR. Interleukin 8 and venous thrombosis:evidence for a role of inflammation in thrombosis. Br J Haematol, 2002, 116(1):173-177.
9.	Kaplanski G, Fabrigoule M, Boulay V, et al. Thrombin induces endothelial type Ⅱ activation in vitro:IL-1 and TNF-alpha-independent IL-8 secretion and E-selectin expression. J Immunol, 1997, 158(11):5435-5441.
10.	Wan MX, Zhang XW, T?rkvist L, et al. Low molecular weight heparin inhibits tumor necrosis factor alpha-induced leukocyte rolling. Inflamm Res, 2001, 50(12):581-584.
11.	Celasco G, Moro L, Bozzella R, et al. Efficacy of intracolonic administration of low-molecular-weight heparin CB-01-05, compared to other low-molecular-weight heparins and unfractionated heparin, in experimentally induced colitis in rat. Dig Dis Sci, 2008, 53(12):3170-3175.
12.	Papa A, Danese S, Gasbarrini A, et al. Review article:potential therapeutic applications and mechanisms of action of heparin in inflammatory bowel disease. Aliment Pharmacol Ther, 2000, 14(11):1403-1409.
13.	Otsuka M, Tsuchiya S, Aramaki Y. Comparison of inhibitory effects of polyanions on nitric oxide production by macrophages stimulated with LPS. Biol Pharm Bull, 2006, 29(3):499-502.
14.	Salas A, Sans M, Soriano A, et al. Heparin attenuates TNF-alpha induced inflammatory response through a CD11b dependent mechanism. Gut, 2000, 47(1):88-96.
15.	孫翠萍, 馬建軍, 李學, 等.無癥狀性腦梗死患者高敏C-反應蛋白、同型半胱氨酸、纖維蛋白原和血脂成分分析.中華實用診斷與治療雜志, 2009, 23(1):32-34.
16.	Van Deventer SJ. Tumor necrosis factor and Crohn's disease. Gut, 1997, 40(4):443-448.
17.	Backer S, Derlin RB, Haskill JS. Differential production of tumor necrosis factor macrophage colony stimulating factor and interleukin-1 by human alveolar macrophages. J Leukol Biol, 1989, 45(4):353-361.
18.	林春繁.低分子肝素治療新生兒膿毒癥并彌散性血管內凝血的臨床研究.國際醫藥衛生導報, 2014, 20(1):55-57.
19.	武夢驊.低分子肝素治療新生兒膿毒癥合并DIC 48例臨床療效觀察.淮海醫藥, 2015, 33(5):182-183.

1. 黨曉衛, 姚振濤, 喬師師, 等.兔下腔靜脈球囊損傷后血管重塑相關因子的變化及G-CSF的干預作用.中國普通外科雜志, 2013, 22(6):721-725.
2. White RH. The epidemiology of venous thromboembolism. Circulation, 2003, 107(23 Suppl 1):14-18.
3. 關振鵬, 呂厚山.外科相關靜脈血栓栓塞的防治.中國臨床醫生, 2009, 37(9):5-8.
4. 曹化, 居克舉, 倪桂華, 等.彈力襪預防出血性卒中患者下肢深靜脈血栓形成的有效性及安全性觀察.中國全科醫學, 2011, 14(3):333-335.
5. Lytvyn OI, Kopitsa MP, Petyunina OV. Interaction between inflammation and thrombosis in acute coronary syndrome. Kardiol Pol, 2004, 61(8):110-116.
6. Salobir B, Sabovic M. Possible vascular-bed-specific role of interleukin-6 in young women with a history of myocardial infarction, lacunar cerebral infarction and deep vein thrombosis. Cytokine, 2004, 25(6):265-272.
7. Esmon CT. The impact of the inflammatory response on coagulation. Thromb Res, 2004, 114(5):321-327.
8. van Aken BE, Reitsma PH, Rosendaal FR. Interleukin 8 and venous thrombosis:evidence for a role of inflammation in thrombosis. Br J Haematol, 2002, 116(1):173-177.
9. Kaplanski G, Fabrigoule M, Boulay V, et al. Thrombin induces endothelial type Ⅱ activation in vitro:IL-1 and TNF-alpha-independent IL-8 secretion and E-selectin expression. J Immunol, 1997, 158(11):5435-5441.
10. Wan MX, Zhang XW, T?rkvist L, et al. Low molecular weight heparin inhibits tumor necrosis factor alpha-induced leukocyte rolling. Inflamm Res, 2001, 50(12):581-584.
11. Celasco G, Moro L, Bozzella R, et al. Efficacy of intracolonic administration of low-molecular-weight heparin CB-01-05, compared to other low-molecular-weight heparins and unfractionated heparin, in experimentally induced colitis in rat. Dig Dis Sci, 2008, 53(12):3170-3175.
12. Papa A, Danese S, Gasbarrini A, et al. Review article:potential therapeutic applications and mechanisms of action of heparin in inflammatory bowel disease. Aliment Pharmacol Ther, 2000, 14(11):1403-1409.
13. Otsuka M, Tsuchiya S, Aramaki Y. Comparison of inhibitory effects of polyanions on nitric oxide production by macrophages stimulated with LPS. Biol Pharm Bull, 2006, 29(3):499-502.
14. Salas A, Sans M, Soriano A, et al. Heparin attenuates TNF-alpha induced inflammatory response through a CD11b dependent mechanism. Gut, 2000, 47(1):88-96.
15. 孫翠萍, 馬建軍, 李學, 等.無癥狀性腦梗死患者高敏C-反應蛋白、同型半胱氨酸、纖維蛋白原和血脂成分分析.中華實用診斷與治療雜志, 2009, 23(1):32-34.
16. Van Deventer SJ. Tumor necrosis factor and Crohn's disease. Gut, 1997, 40(4):443-448.
17. Backer S, Derlin RB, Haskill JS. Differential production of tumor necrosis factor macrophage colony stimulating factor and interleukin-1 by human alveolar macrophages. J Leukol Biol, 1989, 45(4):353-361.
18. 林春繁.低分子肝素治療新生兒膿毒癥并彌散性血管內凝血的臨床研究.國際醫藥衛生導報, 2014, 20(1):55-57.
19. 武夢驊.低分子肝素治療新生兒膿毒癥合并DIC 48例臨床療效觀察.淮海醫藥, 2015, 33(5):182-183.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

低分子肝素鈣對急性深靜脈血栓大鼠血清IL-6和TNF-α水平的影響

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