Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 閆文貌 ,  程石

首都醫科大學附屬北京天壇醫院普通外科（北京100050）;

Corresponding?author：

程石, Email: sh_cheng@hotmail.com

Keywords：

重癥急性胰腺炎; 肺泡巨噬細胞; FasL蛋白; 凋亡; 氯化釓

DOI：

10.7507/1007-9424.20140205

Video：

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目的探討FasL在重癥急性胰腺炎（SAP）大鼠肺泡巨噬細胞（AM）凋亡機理中的作用。方法 SD大鼠按數字表法隨機分為對照組、SAP組及氯化釓（GdCl₃）組3組，每組16只。SAP模型制成6 h后，經支氣管肺泡灌洗獲取AM。取右肺下葉行HE染色檢查，透射電鏡觀察和雙染色法檢測AM凋亡情況，用蛋白免疫印跡法（Western blot法）檢測各組AM中FasL蛋白表達水平。結果 GdCl₃組電鏡下可見AM典型凋亡形態學特征，AM凋亡率為（22.48±1.44）%，明顯高于對照組〔（11.28±1.01）%〕及SAP組〔（6.86±1.35）%〕，其差異有統計學意義（P＜0.05）；GdCl₃組FasL蛋白相對表達量為（1.230±0.041）%，較對照組〔（0.936±0.024）%〕和SAP組〔（0.704±0.011）%〕明顯增高（P＜0.05）。AM凋亡率與AM中FasL蛋白表達水平呈線性正相關關系（R²=0.766，P＜0.01）。結論 GdCl₃可能通過激活FasL蛋白表達誘導SAP大鼠AM發生凋亡。

Citation： 閆文貌, 程石. Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(7): 869-871. doi: 10.7507/1007-9424.20140205 Copy

1.	?Takeda K, Mikami Y, Fukuyama S, et al. Pancreatic ischemia associated with vasospasm in the early phase of human acute necrotizing pancreatitis[J]. Pancreas, 2005, 30(1):40-49.
2.	閆文貌, 程石, 韓穎.氯化釓對重癥急性壞死性胰腺炎大鼠肺損傷的防護作用及機制[J].山東醫藥, 2008, 48(29):19-21.
3.	程石, 何三光, 張佳林.肺泡巨噬細胞活化在急性壞死性胰腺炎大鼠肺損傷中的作用[J].中華外科雜志, 2002, 40(8): 609-612.
4.	中華醫學會外科分會胰腺外科學組.重癥急性胰腺炎診治指南[J].中華外科雜志, 2007, 45(11):727-729.
5.	Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995, 269(5 Pt 1):C1295-C1304.
6.	Saluja A, Hofbauer B, Yamaguchi Y, et al. Induction of apoptosis reduces the severity of caerulein-induced pancreatitis in mice[J]. Biochem Biophys Res Commun, 1996, 220(3):875-878.
7.	張桂信, 陳海龍, 宮愛霞, 等.胰腺腺泡細胞凋亡于急性胰腺炎及治療策略[J].世界華人消化雜志, 2007, 15(10):1115-1120.
8.	Bhatia M. Apoptosis of pancreatic acinar cells in acute panereatitis:is it good or bad?[J]. J Cell Mol Med, 2004, 8(3):402-409.
9.	程石, 宋茂民, 史敬東.氯化釓對急性壞死性胰腺炎肺泡巨噬細胞分泌炎癥介質的影響[J].中國普通外科雜志, 2006, 15(11):836-839.
10.	Mao H, Liu Q, Zhang J, et al. Effects of specific antisense oligonucleotide inhibition of Fas expression on T cell apoptosis induced by Fas ligand[J]. Int Immunopharmacol, 2007, 7(13):1714-1722.
11.	Chang HW, Jeng CR, Lin CM, et al. The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocvte apoptosis invitro[J]. Vet Microbiol, 2007, 122(1-2):72-82.
12.	Uehara S, Gothoh K, Handa H, et al. Immune function in patients with acute pancreatitis[J]. J Gastroenteml Hepatol, 2003, 18(4):363-370.
13.	Kitsmura Y, Hashimoto S, Mizuta N, et al. Fas/FasL-dependent apoptosis of a1veolar cells after lipopolysaccharide-induced lung injury in mice[J]. Am J Respir Crit Care Med, 200l, 163(3 Pt l): 762-769.
14.	Peng Y, Gallagher SF, Haines K, et al. Nuclear factor-kappaB mediates Kupffer cell apoptosis through transcriptional activation of Fas/FasL[J]. J Surg Res, 2006, 130(1):58-65.
15.	Gallagher SF, Peng Y, Haines K, et al. Fas/FasL play a central role in pancreatitis-induced hepatocyte apoptosis[J]. J Gastrointest Surg, 2005, 9(4):467-475.
16.	Gallagher SF, Yang J, Baksh K, et al. Acute pancreatitis induces FasL gene expression and apoptosis in the liver[J]. J Surg Res, 2004, 122(2) :201-209.

1. ?Takeda K, Mikami Y, Fukuyama S, et al. Pancreatic ischemia associated with vasospasm in the early phase of human acute necrotizing pancreatitis[J]. Pancreas, 2005, 30(1):40-49.
2. 閆文貌, 程石, 韓穎.氯化釓對重癥急性壞死性胰腺炎大鼠肺損傷的防護作用及機制[J].山東醫藥, 2008, 48(29):19-21.
3. 程石, 何三光, 張佳林.肺泡巨噬細胞活化在急性壞死性胰腺炎大鼠肺損傷中的作用[J].中華外科雜志, 2002, 40(8): 609-612.
4. 中華醫學會外科分會胰腺外科學組.重癥急性胰腺炎診治指南[J].中華外科雜志, 2007, 45(11):727-729.
5. Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995, 269(5 Pt 1):C1295-C1304.
6. Saluja A, Hofbauer B, Yamaguchi Y, et al. Induction of apoptosis reduces the severity of caerulein-induced pancreatitis in mice[J]. Biochem Biophys Res Commun, 1996, 220(3):875-878.
7. 張桂信, 陳海龍, 宮愛霞, 等.胰腺腺泡細胞凋亡于急性胰腺炎及治療策略[J].世界華人消化雜志, 2007, 15(10):1115-1120.
8. Bhatia M. Apoptosis of pancreatic acinar cells in acute panereatitis:is it good or bad?[J]. J Cell Mol Med, 2004, 8(3):402-409.
9. 程石, 宋茂民, 史敬東.氯化釓對急性壞死性胰腺炎肺泡巨噬細胞分泌炎癥介質的影響[J].中國普通外科雜志, 2006, 15(11):836-839.
10. Mao H, Liu Q, Zhang J, et al. Effects of specific antisense oligonucleotide inhibition of Fas expression on T cell apoptosis induced by Fas ligand[J]. Int Immunopharmacol, 2007, 7(13):1714-1722.
11. Chang HW, Jeng CR, Lin CM, et al. The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocvte apoptosis invitro[J]. Vet Microbiol, 2007, 122(1-2):72-82.
12. Uehara S, Gothoh K, Handa H, et al. Immune function in patients with acute pancreatitis[J]. J Gastroenteml Hepatol, 2003, 18(4):363-370.
13. Kitsmura Y, Hashimoto S, Mizuta N, et al. Fas/FasL-dependent apoptosis of a1veolar cells after lipopolysaccharide-induced lung injury in mice[J]. Am J Respir Crit Care Med, 200l, 163(3 Pt l): 762-769.
14. Peng Y, Gallagher SF, Haines K, et al. Nuclear factor-kappaB mediates Kupffer cell apoptosis through transcriptional activation of Fas/FasL[J]. J Surg Res, 2006, 130(1):58-65.
15. Gallagher SF, Peng Y, Haines K, et al. Fas/FasL play a central role in pancreatitis-induced hepatocyte apoptosis[J]. J Gastrointest Surg, 2005, 9(4):467-475.
16. Gallagher SF, Yang J, Baksh K, et al. Acute pancreatitis induces FasL gene expression and apoptosis in the liver[J]. J Surg Res, 2004, 122(2) :201-209.

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Role of FasL Expression in The Apoptosis of Alveolar Macrophages of Severe Acute Pancreatitis

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