Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

XUShi-jun ,  MUJun-sheng , ZHANGJian-qun , BOPing

Department of Cardiac Surgery, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Capital Medical University, Beijing 100029, P. R. China;

Corresponding?author：

MUJun-sheng, Email: wesleymu@hotmail.com

Keywords：

Mouse induced pluripotent stem cells; Myocardial Patches; Poly-3-hydroxybutyrate-co-3-hydroxyhex-anoate(PHBHHx)film; Stem cell therapy

DOI：

10.7507/1007-4848.20160196

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the external biocompatibility bewteen the mouse induced pluripotent stem cells (miPSCs) and poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx). Methods After we recovered and subcultured miPSCs, we divided them into two groups. There was one group cultured with material of PHBHHx films outside the body. We observed the adhesive pattern of miPSCs on film by fluorescence of 4, 6-diamidino-2-phenylindole (DAPI) staining. The cell vitality was detected by cell counting kit-8 (CCK-8). The morphology of miPSCs attached on the film was visualized under scanning electron microscope (SEM). We used the traditional petri dish to culture miPSCs and detect the cell activity by CCK-8. Results MiPSCs can adhere and proliferate on PHBHHx films. The result of cell vitality which detected by CCK-8 showed that there was a statistical difference in OD value between culturing on PHBHHx films and traditional cultivation (0.617±0.019 vs. 0.312±0.004, P < 0.05). Conclusion There are adhesion and proliferation on the surface of cells patch made by miPSCs co-culturing with PHBHHx film. Compared with traditional culturing in the cell culture dish, culturing in PHBHHx films have great advantages in the process of adhesion and proliferation. PHBHHx can be used as one of the scaffold for stem cells treating various disease.

Citation： XUShi-jun, MUJun-sheng, ZHANGJian-qun, BOPing. Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2016, 23(8): 822-826. doi: 10.7507/1007-4848.20160196 Copy

1.	穆軍升, 李獻帥, 袁樹民, 等.直接貼壁法誘導人胚胎干細胞分化為心肌細胞.中國胸心血管外科臨床雜志, 2013, 20(5):564-569.
2.	李獻帥, 袁樹民, 穆軍升, 等.懸浮培養誘導人胚胎干細胞分化為心肌細胞的研究.心肺血管病雜志, 2014, 33(1):93-97.
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6.	Yoshida Y, Yamanaka S. iPS cells:a source of cardiac regeneration. J Mol Cell Cardiol, 2011, 50(2):327-332.
7.	牛紅星, 穆軍升, 張健群, 等.骨髓間充質干細胞與聚3-羥基丁酸酯-co-4-羥基丁酸酯生物材料共培養細胞補片的初步研究.中國生物工程雜志, 2012, 32(10):74-79.
8.	Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-capro-lactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(4):5.
9.	Jain K, Verma PJ, Liu J. Isolation and handling of mouse embryonic fibroblasts. Methods Mol Biol, 2014, 1194(6):247-252.
10.	呂海俠, 楊志倩, 盧曉云, 等. PHBHHx膜表面親水改性及其與神經干細胞生物相容性.生物工程學報, 2012, 28(10):1216-1226.
11.	Matar AA, Chong JJ. Stem cell therapy for cardiac dysfunction. Springerplus, 2014, 3(3):440.
12.	劉麗, 張海波.誘導多能干細胞體外分化為心肌細胞研究現狀.中華臨床醫師雜志(電子版), 2012, 28(10):1216-1226.
13.	Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007, 318(5858):1917-1920.
14.	Eminli S, Utikal J, Arnold K, et al. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression. Stem Cells, 2008, 26(10):2467-2474.
15.	Nelson TJ, Martinez-Fernandez A, Yamada S, et al. Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation, 2009, 120(5):408-416.
16.	Li X, Zhang F, Song G, et al. Intramyocardial injection of pig pluri-potent stem cells improves left ventricular function and perfusion:A dtudy in a porcine model of scute myocardial infarction. PLoS One, 2013, 8(6):e66688.
17.	Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
18.	Wu S, Liu Y, Cui B, et al. Intravascular biocompatibility of decellu-larized xenogenic vascular scaffolds/PHBHHx hybrid material for cardiovascular tissue engineering. Sheng Wu Gong Cheng Xue Bao, 2008, 24(4):610-616.
19.	Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials, 2005, 26(33):6565-6578.
20.	Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(6):5.

1. 穆軍升, 李獻帥, 袁樹民, 等.直接貼壁法誘導人胚胎干細胞分化為心肌細胞.中國胸心血管外科臨床雜志, 2013, 20(5):564-569.
2. 李獻帥, 袁樹民, 穆軍升, 等.懸浮培養誘導人胚胎干細胞分化為心肌細胞的研究.心肺血管病雜志, 2014, 33(1):93-97.
3. Kovacic JC, Fuster V. Cell therapy for patients with acute myocar-dial infarction:accured evidence to date. Circ Res, 2015, 116(8):1287-1290.
4. Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
5. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4):663-676.
6. Yoshida Y, Yamanaka S. iPS cells:a source of cardiac regeneration. J Mol Cell Cardiol, 2011, 50(2):327-332.
7. 牛紅星, 穆軍升, 張健群, 等.骨髓間充質干細胞與聚3-羥基丁酸酯-co-4-羥基丁酸酯生物材料共培養細胞補片的初步研究.中國生物工程雜志, 2012, 32(10):74-79.
8. Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-capro-lactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(4):5.
9. Jain K, Verma PJ, Liu J. Isolation and handling of mouse embryonic fibroblasts. Methods Mol Biol, 2014, 1194(6):247-252.
10. 呂海俠, 楊志倩, 盧曉云, 等. PHBHHx膜表面親水改性及其與神經干細胞生物相容性.生物工程學報, 2012, 28(10):1216-1226.
11. Matar AA, Chong JJ. Stem cell therapy for cardiac dysfunction. Springerplus, 2014, 3(3):440.
12. 劉麗, 張海波.誘導多能干細胞體外分化為心肌細胞研究現狀.中華臨床醫師雜志(電子版), 2012, 28(10):1216-1226.
13. Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007, 318(5858):1917-1920.
14. Eminli S, Utikal J, Arnold K, et al. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression. Stem Cells, 2008, 26(10):2467-2474.
15. Nelson TJ, Martinez-Fernandez A, Yamada S, et al. Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation, 2009, 120(5):408-416.
16. Li X, Zhang F, Song G, et al. Intramyocardial injection of pig pluri-potent stem cells improves left ventricular function and perfusion:A dtudy in a porcine model of scute myocardial infarction. PLoS One, 2013, 8(6):e66688.
17. Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
18. Wu S, Liu Y, Cui B, et al. Intravascular biocompatibility of decellu-larized xenogenic vascular scaffolds/PHBHHx hybrid material for cardiovascular tissue engineering. Sheng Wu Gong Cheng Xue Bao, 2008, 24(4):610-616.
19. Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials, 2005, 26(33):6565-6578.
20. Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(6):5.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body

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