Effect of MUC1 Over-expression on Chemotherapy of 5-Fluorouracil and Cisplatin for Esophageal Cancer Cells_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHENGMin ^1,2 , YEQing ³ , YANGCheng-guang ² , XUYe ² , GUOMing ² ,  XUZhi-fei ¹

1. Department of Thoracic Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200061, P. R. China;
2. Department of Thoracic Surgery, Shanghai Changning District Central Hospital, Shanghai 200336, P. R. China;
3. Department of Thoracic Surgery, Shanghai Renji Hospital, School of Medicine of Shanghai Jiaotong University, Shanghai 201210, P. R. China;

Corresponding?author：

XUZhi-fei, Email: doctorxzf@163.com

Keywords：

Esophageal cancer; MUC1; Cisplatin; 5-Fluorouracil; Nude mice

DOI：

10.7507/1007-4848.20140107

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Objective To investigate MUC1 over-expression on chemotherapy of 5-fluorouracil and cisplatin for esophageal cancer cells. Methods MUC1 over-expression and stable silencing of MUC1 expression esophageal cancer cell lines were constructed. Xenograft model of esophageal cancer was established in nude mice. Cisplatin (8 mg/kg, day 1 and day 7)and 5-fluorouracil (20 mg/kg, day 1 to 6)were injected intraperitoneally. Tumor volume and body weight of nude mice were measured. Tumor growth curve and body weight curve were drawn, and tumor inhibitory rate was calculated. Results Both cisplatin and 5-fluorouracil suppressed tumor growth of MUC1 over-expression esophageal cancer nude mice. Body weight and tumor volume of nude mice of cisplatin and 5-fluorouracil groups were significantly smaller than those of the control group (P < 0.05), and the inhibitory effects of cisplatin were significantly greater than those of 5-fluorouracil (P < 0.05). There was no significant inhibitory effect in stable silencing of MUC1 expression esophageal cancer nude mice. Conclusion Both cisplatin and paclitaxel can suppress the growth of MUC1 over-expression esophageal cancer, and cisplatin has greater inhibitory effects than 5-fluorouracil in tumor volume and body weight of nude mice.

Citation： ZHENGMin, YEQing, YANGCheng-guang, XUYe, GUOMing, XUZhi-fei. Effect of MUC1 Over-expression on Chemotherapy of 5-Fluorouracil and Cisplatin for Esophageal Cancer Cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2014, 21(3): 394-398. doi: 10.7507/1007-4848.20140107 Copy

1.	Creaney J, Segal A, Sterrett G, et al. Overexpression and altered glycosylation of MUCl in malignant mesothelioma. Br J Cancer, 2008, 98 (9):1562-1569.
2.	王立順, 朱迅. MUC1和MUC2 mRNA在不同腫瘤組織的表達及其意義.中國腫瘤臨床, 2000, 27 (4):258-261.
3.	于秀文, 李淑霞, 馮美燕, 等.黏蛋白MUC1和MUC2聯合表達與大腸癌的相關性.臨床與實驗病理學雜志, 2004, 20 (5):611-614.
4.	趙立明, 王永翔, 劉學軍, 等.膀胱尿路上皮細胞癌MUC1和MUC2蛋白的表達及臨床意義.中國臨床醫學, 2009, 16 (2):239-241.
5.	Nozoe T, Ezaki T, Kabashima A, et al. Significance of immunohistochemical expression of cyclooxygenase-2 in squamous cell carcinoma of the esophagus. Am J Surg, 2005, 189 (1):110-115.
6.	逄旭光, 范虹, 葛棣. HER4基因抑制對食管癌細胞Eca-109遷移和侵襲能力的影響.復旦學報:醫學版, 2008, 35 (4):521-527.
7.	趙璇, 李沛, 馬俊芬, 等. TIMP3、E-Cadherin基因在食管癌細胞系EC1和EC9706中的甲基化及蛋白表達.世界華人消化雜志, 2010, 18 (4):379-383.
8.	Feng JG, Liu Q, Qin X, et al. Clinicopathological pattern and Annexin A2 and Cdc42 status in patients presenting with differen tiation and lymphnode metastasis of esophageal squamous cell carcinomas. Mol Biol Rep, 2012, 39 (2):1267-1274.
9.	趙意, 蔡開燦, 劉德綱, 等.恩度聯合TP方案對食管癌細胞抑制作用的實驗研究.實用醫學雜志, 2012, 28 (12):1963-1965.
10.	Guang W, Czinn SJ, Blanchard TG, et al. Genetic regulation of MUC1 expression by Helicobacter pylori in gastric cancer cells. Biochem Biophys Res Commun, 2014, 445 (1)145-150.
11.	Lavrsen K, Madsen CB, Rasch MG, et al. Aberrantly glycosylated MUC1 is expressed on the surface of breast cancer cells and a target for antibody-dependent cell-mediated cytotoxicity. Glycoconj J, 2013, 30 (3):227-236.
12.	Deng JL, Wang L, Chen HM, et al. The role of tumour-asso ciated MUC1 in epithelial ovarian Cancer metastasis and progression. Cancer Metastasis Rev, 2013, 32 (3/4):535-551.
13.	Suzuki Y, Sutoh M, Hatakeyama S, et al. MUC1 carrying core 2 O-glycans functions as a molecular shield against NK cell attack, promoting bladder tumor metastasis. Int J Oncol, 2012, 40 (6):1831-1838.
14.	翁羽蕙, 張鈾. MUCl的研究進展.重慶醫學, 2010, 39 (10):1288-1290.
15.	Chou Tc. Theoretical basis, experimental design, and compu-terized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev, 2006, 58 (3):621-681.
16.	蘇翔宇, 李蘇宜, 劉琳.奈達鉑聯合順鉑對人食管癌細胞株(Eca-109)抑制作用機制的研究.實用腫瘤雜志, 2010, 25 (2):150-155.
17.	Jin Y, Li J, Rong LF, et al. Anti-hepatocarcinoma effects of 5-fluorouracil encapsulated by galactosylceramide liposomes in vivo and in vitro. World J Gastroenterol, 2005, 11 (17):2643-2646.
18.	Abdi K, Khalaj A, Ostad SN, et al. Cytotoxicity and radiosensitising activity of synthesized dinitrophenyl derivatives of 5-fluorouracil. Daru, 2012, 20 (1):3.
19.	Milross CG, Mason KA, Hunter NR, et al. Relationship of mitotic arrest and apoptosis to antitu-mor effete of paelitaxel. Natl Cancer Inst, 1996, 88 (18):1308-1314.
20.	Uguz AC, Cig B, Espino J, et al. Melatonin potentiates chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor cells. J Pineal Res, 2012, 53 (1):91-98.

1. Creaney J, Segal A, Sterrett G, et al. Overexpression and altered glycosylation of MUCl in malignant mesothelioma. Br J Cancer, 2008, 98 (9):1562-1569.
2. 王立順, 朱迅. MUC1和MUC2 mRNA在不同腫瘤組織的表達及其意義.中國腫瘤臨床, 2000, 27 (4):258-261.
3. 于秀文, 李淑霞, 馮美燕, 等.黏蛋白MUC1和MUC2聯合表達與大腸癌的相關性.臨床與實驗病理學雜志, 2004, 20 (5):611-614.
4. 趙立明, 王永翔, 劉學軍, 等.膀胱尿路上皮細胞癌MUC1和MUC2蛋白的表達及臨床意義.中國臨床醫學, 2009, 16 (2):239-241.
5. Nozoe T, Ezaki T, Kabashima A, et al. Significance of immunohistochemical expression of cyclooxygenase-2 in squamous cell carcinoma of the esophagus. Am J Surg, 2005, 189 (1):110-115.
6. 逄旭光, 范虹, 葛棣. HER4基因抑制對食管癌細胞Eca-109遷移和侵襲能力的影響.復旦學報:醫學版, 2008, 35 (4):521-527.
7. 趙璇, 李沛, 馬俊芬, 等. TIMP3、E-Cadherin基因在食管癌細胞系EC1和EC9706中的甲基化及蛋白表達.世界華人消化雜志, 2010, 18 (4):379-383.
8. Feng JG, Liu Q, Qin X, et al. Clinicopathological pattern and Annexin A2 and Cdc42 status in patients presenting with differen tiation and lymphnode metastasis of esophageal squamous cell carcinomas. Mol Biol Rep, 2012, 39 (2):1267-1274.
9. 趙意, 蔡開燦, 劉德綱, 等.恩度聯合TP方案對食管癌細胞抑制作用的實驗研究.實用醫學雜志, 2012, 28 (12):1963-1965.
10. Guang W, Czinn SJ, Blanchard TG, et al. Genetic regulation of MUC1 expression by Helicobacter pylori in gastric cancer cells. Biochem Biophys Res Commun, 2014, 445 (1)145-150.
11. Lavrsen K, Madsen CB, Rasch MG, et al. Aberrantly glycosylated MUC1 is expressed on the surface of breast cancer cells and a target for antibody-dependent cell-mediated cytotoxicity. Glycoconj J, 2013, 30 (3):227-236.
12. Deng JL, Wang L, Chen HM, et al. The role of tumour-asso ciated MUC1 in epithelial ovarian Cancer metastasis and progression. Cancer Metastasis Rev, 2013, 32 (3/4):535-551.
13. Suzuki Y, Sutoh M, Hatakeyama S, et al. MUC1 carrying core 2 O-glycans functions as a molecular shield against NK cell attack, promoting bladder tumor metastasis. Int J Oncol, 2012, 40 (6):1831-1838.
14. 翁羽蕙, 張鈾. MUCl的研究進展.重慶醫學, 2010, 39 (10):1288-1290.
15. Chou Tc. Theoretical basis, experimental design, and compu-terized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev, 2006, 58 (3):621-681.
16. 蘇翔宇, 李蘇宜, 劉琳.奈達鉑聯合順鉑對人食管癌細胞株(Eca-109)抑制作用機制的研究.實用腫瘤雜志, 2010, 25 (2):150-155.
17. Jin Y, Li J, Rong LF, et al. Anti-hepatocarcinoma effects of 5-fluorouracil encapsulated by galactosylceramide liposomes in vivo and in vitro. World J Gastroenterol, 2005, 11 (17):2643-2646.
18. Abdi K, Khalaj A, Ostad SN, et al. Cytotoxicity and radiosensitising activity of synthesized dinitrophenyl derivatives of 5-fluorouracil. Daru, 2012, 20 (1):3.
19. Milross CG, Mason KA, Hunter NR, et al. Relationship of mitotic arrest and apoptosis to antitu-mor effete of paelitaxel. Natl Cancer Inst, 1996, 88 (18):1308-1314.
20. Uguz AC, Cig B, Espino J, et al. Melatonin potentiates chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor cells. J Pineal Res, 2012, 53 (1):91-98.

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