Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice_West China Medical Journal

Authors：

1. Department of Pathology, North Sichuan Medical College & Department of Pathology, Affiliated Hospital of North Sichuan Medical College; 1. Department of Otolaryngology-Head and Neck Surgery, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College; 3. Institute of Molecular Biology, North Sichuan Medical College; Nanchong, Sichuan 637007, P. R. China;

Corresponding?author：

Keywords：

ATM基因; 反義ATM; 喉鱗狀細胞癌

DOI：

10.7507/1002-0179.20130226

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　探討反義ATM多核苷酸（AS-Lipo）作用于裸鼠移植瘤對喉鱗狀細胞癌(Hep-2)放射治療敏感性的影響。方法　用克隆生存率來檢測喉鱗狀細胞癌放射治療后的生存能力；用流式細胞儀來分析喉癌細胞的凋亡情況；用Tunel染色法檢測各組瘤體中細胞凋亡情況。結果　在同等劑量的放射治療下，AS-Lipo組的生存分數最低。接受4 Gy放射治療后，行流式細胞儀檢測，結果發現AS-Lipo組的凋亡率為（30.7 ± 1.31）%，遠高于轉染Sen-Lipo、Mis-Lipo、Lipo組。ATM AS-Lipo+放療組的細胞凋亡數顯著高于單獨放射治療組；ATM AS-Lipo+放射治療組裸鼠瘤體內的凋亡指數為（17.12 ± 4.2）%，與其他組比較差異有統計學意義。結論　反義ATM多核苷酸增強了裸鼠喉鱗狀細胞癌對放射治療的敏感性。

Citation： LI Li,FENG Jun,WANG Chaoli,JING Baoqian.. Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice. West China Medical Journal, 2013, 28(5): 728-731. doi: 10.7507/1002-0179.20130226 Copy

1.	Hristov B, Bajaj GK. Radiotherapeutic management of laryngeal carcinoma[J]. Otolaryngologic clinics of North America, 2008, 41(4): 715-740.
2.	Li L, Story M, Legerski RJ. Cellular responses to ionizing radiation damage [J]. Int J Radiat Oncol Biol Phys, 2001, 49(4): 1157-1162.
3.	Christopher WJ, Dariel PG, Cecile VRT. Aberrant recombination involving the granzyme locus occurs in Atm-/-T-cell lymphomas[J]. Human Molecular Genetics, 2005, 14(18): 2671-2684.
4.	Helt CE, Cliby WA, Keng PC, et al. Ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein exhibit selective target specificities in response to different forms of DNA damage[J]. J BiolChem, 2005, 280(2): 1186-92.
5.	Kastan MB, Lim DS. The many substrates and functions of ATM[J]. Nat RevMol Cell Biol, 2000, 1(2): 179-186.
6.	Chong MJ, Murray MR, Gosink EC, et al. Atm and Bax cooperate in ionizing radiation induced apoptosis in the central nervous system[J]. Proc Natl Acad Sci USA., 2000, 97(2): 889-894.
7.	Borges HL, Chao C, Xu Y, et al. Radiation-induced apoptosis in developing mouse retina exhibits dose-dependent requirement for ATM phosphorylation of p53[J]. Cell Death Differ, 2004, 11(5): 494-502.
8.	江繼平, 馮俊, 唐嗣泉, 等．反義ATM在體外對喉鱗狀細胞癌ATM蛋白表達影響的研究[J]. 川北醫學院學報, 2011, 26(5): 394-396.
9.	李麗, 馮俊, 王朝莉, 等．反義ATM對喉鱗狀細胞癌ATM mRNA、蛋白影響的體外研究[J]．川北醫學院學報, 2012, 27(5): 438-440.

1. 　Hristov B, Bajaj GK. Radiotherapeutic management of laryngeal carcinoma[J]. Otolaryngologic clinics of North America, 2008, 41(4): 715-740.
2. 　Li L, Story M, Legerski RJ. Cellular responses to ionizing radiation damage [J]. Int J Radiat Oncol Biol Phys, 2001, 49(4): 1157-1162.
3. 　Christopher WJ, Dariel PG, Cecile VRT. Aberrant recombination involving the granzyme locus occurs in Atm-/-T-cell lymphomas[J]. Human Molecular Genetics, 2005, 14(18): 2671-2684.
4. 　Helt CE, Cliby WA, Keng PC, et al. Ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein exhibit selective target specificities in response to different forms of DNA damage[J]. J BiolChem, 2005, 280(2): 1186-92.
5. 　Kastan MB, Lim DS. The many substrates and functions of ATM[J]. Nat RevMol Cell Biol, 2000, 1(2): 179-186.
6. 　Chong MJ, Murray MR, Gosink EC, et al. Atm and Bax cooperate in ionizing radiation induced apoptosis in the central nervous system[J]. Proc Natl Acad Sci USA., 2000, 97(2): 889-894.
7. 　Borges HL, Chao C, Xu Y, et al. Radiation-induced apoptosis in developing mouse retina exhibits dose-dependent requirement for ATM phosphorylation of p53[J]. Cell Death Differ, 2004, 11(5): 494-502.
8. 　江繼平, 馮俊, 唐嗣泉, 等．反義ATM在體外對喉鱗狀細胞癌ATM蛋白表達影響的研究[J]. 川北醫學院學報, 2011, 26(5): 394-396.
9. 　李麗, 馮俊, 王朝莉, 等．反義ATM對喉鱗狀細胞癌ATM mRNA、蛋白影響的體外研究[J]．川北醫學院學報, 2012, 27(5): 438-440.

West China Medical Journal

Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content