Granulomatous lobular mastitis (GLM) is a rare mastitis disease, which mainly occurs in childbearing period. The etiology, diagnosis and treatment of GLM are still disputed. In recent years, the role of Corynebacterium infection in the pathogenesis of GLM has attracted more and more attention. Cystic neutrophilic granulomatous mastitis (CNGM) has been proposed, and more and more studies have targeted CNGM as a unique subtype of GLM. This article summarizes the published literature on CNGM so far to discuss the research progress in clinicopathological features, microbiological findings, clinical diagnosis and treatment of CNGM.
目的 合成可生物降解的基因載體，并分析其生物毒性及轉染率。 方法 低分子量聚乙烯亞胺（PEI）通過雙硫鍵交聯合成可降解的高分子量PEI衍生物（SS-PEI），通過紅外光譜和核磁波譜分析技術分析其化學結構，采用細胞活力實驗和檢測大鼠肝腎功能指標分析其細胞和體內毒性，并轉染羥基熒光素修飾的siRNA（FAM-siRNA）分析細胞轉染率。 結果 紅外波譜和核磁波譜分析可見酰胺鍵的特征波譜，噻唑藍法和肝腎功能指標顯示SS-PEI不同劑量組與對照組的差異均無統計學意義（P＞0.05），SS-PEI/FAM-siRNA轉染率為（76.0 ± 2.8）%。 結論 SS-PEI的合成可明顯提高裝載siRNA的效率，具有安全、高效等特點。