ObjectiveTo investigate the influence of different temperatures of distilled water on the general sensation of patients during endoscopic ultrasonography (EUS) examination. MethodsSixty patients who received EUS examination were randomly assigned to trial group and control group. The trial group used (40±1) ℃ distilled water, while the control group accepted 26-28℃ distilled water. The operating time and the grade of discomfort symptoms such as general discomfort, abdominal pain, nausea, vomiting and terror were observed. ResultsSignificant differences in general discomfort, nausea, vomiting and terror were found between the two groups (P ＜ 0.001) . Abdominal pain was not found in either group. The examination time in each group did not show any significant difference (P ＞ 0.05) . ConclusionDifferent distilled water temperatures significantly influence the sensation of patients during the EUS examination, and distilled water with a temperature of (40±1) ℃ is able to relieve the discomfort of patients.