ObjectiveTo explore the dyeing conditions of reticular fiber staining of liver puncture tissue stained by BenchMark automatic special staining instrument, evaluate the staining effect, and share the using experience.MethodsA total of 30 cases of liver puncture specimens from April to May 2019 in West China Hospital of Sichuan University were selected. They were fixed with 4% neutral formaldehyde fix solution, treated with automatic dehydrator, embedded in paraffin, stained with adhesive slides, and baked in 65℃ oven for 30 min. Each specimen was sliced in duplicate, with a thickness of 5 μm. One was reticular fiber stained with BenchMark automatic special staining instrument under the dyeing condition according to the using experience of the laboratory, and the other was reticular fiber stained by manual Foot staining method according to the standard operating procedure of the laboratory. The staining effect under microscope was observed by a chief pathologist with extensive slide-reading experience.ResultsThe success rate of instrumental method was 100.0% (30/30), and that of the manual method was 76.7% (23/30). The difference in success rate between the two methods was statistically significant (χ2=5.143, P=0.023).ConclusionThe reticular fiber staining effect of BenchMark automatic special staining instrument is stable and reliable, and the success rate is higher than that of manual Foot staining.