PNAS-4 is a novel pro-apoptosis gene identified latetly. In recent years, there has been a large number of research reports on the basic studies about PNAS-4 in cancer gene therapy and gene therapy of PNAS-4 alone or combined with chemotherapy or radiotherapy manifested a good application prospect, but its molecular mechanisms to promote apoptosis is not clear yet. In this paper, recent research about PNAS-4 in cancer gene therapy is briefly reviewed, and recent hypotheses on its molecular mechanisms to promote apoptosis are especially elucidated. Based on its newly identified characteristics of structural domain, we made a point that PNAS-4 might regulate functions of some target protein related to apoptosis by deSumoylation as a new deSumoylating isopeptidase, and consequently promote apoptosis.
目的:探討人凋亡相關新基因PNAS-4(hPNAS-4)基因通過脂質體轉染至Lewis肺癌LL2細胞后對放射治療的增敏作用。〖HTH〗方法〖HTSS〗:用脂質體介導的轉染技術,將hPNAS-4基因導入Lewis肺癌LL2細胞中,Western blot鑒定其過表達后,觀察X射線照射對其集落形成的影響;流式細胞儀檢測hPNAS-4基因或／和放療(0,1,2,4,6 Gy)對LL2細胞生長抑制及凋亡的影響。〖HTH〗結果〖HTSS〗:通過Western blot證實了hPNAS-4基因在LL2細胞中的過表達。Lip-hPNAS-4加放射治療處理組細胞的生存能力明顯降低,腫瘤細胞的克隆形成明顯減少,流式細胞術檢測體外培養的腫瘤細胞凋亡明顯增加。〖HTH〗結論〖HTSS〗:hPNAS-4基因聯合放射治療能產生協同抗腫瘤效應。