人凋亡相關新基因PNAS-4與放射聯合治療Lewis肺癌的實驗研究_《華西醫學》

作者：

曾輝 ¹ , 石華山 ² ,  李志平 ¹

四川大學1.華西醫院腹部腫瘤科;2.國家重點腫瘤生物治療實驗室,四川成都 610041;

關鍵詞：

基因 hPNAS-4 Lewis肺癌放射治療放療敏感性

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目的:探討人凋亡相關新基因PNAS-4(hPNAS-4)基因通過脂質體轉染至Lewis肺癌LL2細胞后對放射治療的增敏作用。〖HTH〗方法〖HTSS〗:用脂質體介導的轉染技術,將hPNAS-4基因導入Lewis肺癌LL2細胞中,Western blot鑒定其過表達后,觀察X射線照射對其集落形成的影響;流式細胞儀檢測hPNAS-4基因或／和放療(0,1,2,4,6 Gy)對LL2細胞生長抑制及凋亡的影響。〖HTH〗結果〖HTSS〗:通過Western blot證實了hPNAS-4基因在LL2細胞中的過表達。Lip-hPNAS-4加放射治療處理組細胞的生存能力明顯降低,腫瘤細胞的克隆形成明顯減少,流式細胞術檢測體外培養的腫瘤細胞凋亡明顯增加。〖HTH〗結論〖HTSS〗:hPNAS-4基因聯合放射治療能產生協同抗腫瘤效應。

引用本文： 曾輝,石華山,李志平. 人凋亡相關新基因PNAS-4與放射聯合治療Lewis肺癌的實驗研究. 華西醫學, 2009, 24(4): 926-929. doi: 復制

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1. HUH J J, WOLF J K, FIGHTMASTER D L, et al. Transduction of adenovirus-mediated wild-type p53 after radiotherapy in human cervical cancer cells[J]. Gynecol Oncol,2003,89:243-250..
2. SAH N K, MUNSHI A, NISHIKAWA T, et al. Adenovirus-mediated wild-type p53 radiasensitizes human tumor cells by suppressing DNA repair capacity[J]. Mol Cancer Ther,2003,2:1223-1231.
3. LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
4. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
5. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
6. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
7. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
8. YANG F(楊帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. In vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
9. YANG F(楊帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. In vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
10. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
11. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
12. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
13. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
14. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694.
15. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694..
16. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
17. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
18. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
19. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
20. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
21. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
22. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.[3]LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
23. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.

《華西醫學》

人凋亡相關新基因PNAS-4與放射聯合治療Lewis肺癌的實驗研究

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《華西醫學》

人凋亡相關新基因PNAS-4與放射聯合治療Lewis肺癌的實驗研究

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