Objective To investigate the efficacy of phloretin combined with sodium hyaluronate in preventing postoperative abdominal adhesion formation in rats and its possible mechanisms. Methods Forty rats were randomly divided into five groups, the rats in the sham-operatinon group only underwent open and closed abdominal surgery, and the remaining rats of four groups underwent cecum scratch-and-rub method of modeling to receive different treatments: the rats in the control group and the phloretin group (PHL group) were closed abdominally after modeling, while the rats in the sodium hyaluronate group (HA group) and the phloretin combined with sodium hyaluronate group (PHL+HA group) were closed abdominally by using 2 mL of sodium hyaluronate gel coated with the damaged abdominal wall and the cecum; the postoperative groups treated with phloretin (the PHL and PHL+HA groups) were treated with 2 mL of40 mg/kg phloretin dissolved in 0.5% sodium carboxymethylcellulose by gavage daily, and the rest of the groups were treated with 2 mL of 0.5% sodium carboxymethylcellulose solution by gavage. After general anesthesia, the rats were executed on the 7th day after surgery, and the Nair’s score was used to evaluate the adhesion status of each group on the 7th day after surgery; the adhesive tissue or normal peritoneal tissue were collected (cecum and its opposite side of the peritoneal tissue was collected in the sham-operation group), and immunohistochemistry was performed to evaluate the degree of staining with Nrf2 antibody, HE staining was performed to evaluate the inflammation scores, and Sirius red staining was performed to evaluate the thickness of the collagen fibers, and levels of transforming growth factor β1 (TGF-β1), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. Results All rats successfully completed the experiment. Compared with the control group, Nair’s score, inflammation score, expression level of TGF-β1, thickness of collagen fibers in the adherent tissues, and MDA level were significantly lower in the PHL+HA group (P<0.05), but the SOD level and expression lever of Nrf2 were significantly higher in the PHL+HA group (P<0.05). Conclusion Phloretin combined with sodium hyaluronate can prevent the formation of postoperative abdominal adhesions in the rat model, which may be related to reducing inflammation, reducing collagen deposition, activating Nrf2 pathway and inhibiting oxidative stress.
【摘要】 目的 研究過氧化酶增殖因子活化受體γ（peroxisome proliferator-activated receptor gamma， PPARγ）和血管內皮生長因子C（vascular endothelial growth factor C， VEGF-C）在胃癌組織中表達的相關性，分析其表達與臨床病理因素之間的關系。 方法 2005年3月-2009年3月間采用逆轉錄-聚合酶鏈反應方法檢測36例胃癌手術標本中PPARγ和VEGF-C mRNA的表達，同時選取相同患者的胃正常組織作為對照。 結果 PPARγ mRNA在胃癌中的表達量高于癌旁正常組織，兩者的差異有統計學意義（P=0.007）；VEGF-C在胃癌中的表達量高于癌旁正常組織，兩者的差異有統計學意義（P=0.004）；PPARγ的表達與VEGF-C表達無關聯性（r=0.135，P=0.414）；PPARγ表達與胃癌組織中浸潤程度有關（χ2=4.620，P=0.032）、淋巴結轉移有關（χ2=15.753，P=0.000）和臨床病理分期有關（χ2=4.610，P=0.032）；VEGF-C表達與胃癌組織中淋巴結轉移有關（χ2=4.729，P=0.030）、遠處轉移有關（χ2=4.064，P=0.044）和臨床病理分期有關（χ2=6.300，P=0.012）。 結論 PPARγ和VEGF-C可能在胃癌新生淋巴管形成中起重要作用，兩者的表達水平與胃癌患者的病情判斷及預后評價密切相關。【Abstract】 Objective To investigate the significance of expressions of peroxisome proliferator-activated receptor gamma (PPARγ) and vascular endothelial growth factor C (VEGF-C) in gastric carcinoma as well as their correlation, and to study the relationship between the expressions and clinicopathologic characteristics of gastric carcinoma. Methods Thirty-six pairs of normal mucosa and cancer specimens were obtained from patients who had undergone gastric operation for primary gastric carcinoma and subjected to reverse transcription-ploymerase chain reaction (RT-PCR) for PPARγ and VEGF-C mRNA detection. Results The positive rate and level of PPARγ mRNA expression were higher in gastric cancer tissues than those in normal gastric mucosa (P=0.007). The positive rate and level of VEGF-C mRNA expression were also higher in gastric cancer tissues than those in normal gastric mucosa (P=0.004). Simultaneously, the expression of PPARγ was not correlated with that of VEGF-C (r=0.135, P=0.414), while the highly productions of PPARγ and VEGF-C in gastric carcinomas were both associated with the lymph node metastasis and the clinical stage (Plt;0.05). Conclusion PPARγ and VEGF-C may play an important role in the lymphangio-genesis of gastric cancer, and united detection of PPARγ and VEGF-C expressions may be correlated with making diagnosis, evaluating prognosis in patients with gastric cancer at the same time.