In order to explore the effect of Sipunculus nudus extract (SNE) on skin wound healing in mice and its mechanism, hemostasis effect of SNE was measured, the mouse skin wound model was established by full-thickness excision. The morphological changes of the wound were observed after the treatment with SNE and the healing rate was measured. The changes of wound histology were observed by hematoxylin eosin (HE) staining, Masson staining and transmission electron microscope (TEM). The expression of cell factors and related proteins was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that the SNE possessed hemostatic function. SNE could obviously improve the healing rate of wound in mouse and shorten time of scab removal compared with the none-treatment (NT) group (P < 0.05).The pathological histology analysis results showed complete epidermal regeneration, with remarkable capillary and collagen fiber observed in the SNE group. The expression level of tumor necrosis factor-α (TNF -α), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in SNE group was significantly lower than that of the NT group on 7 d (P < 0.05). Moreover, compared with the NT group, the gene expressions level of Smad7 was significantly increased and the level of type II TGF-β receptors (TGF-βRII), collagen I (COL1A1) and α-smooth muscle actin (α-SMA) were significantly reduced in the SNE group on 28 d (P < 0.05), but the difference was not statistically significant compared to Yunnanbaiyao group (PC group) (P > 0.05). These results indicated that SNE possessed obvious activity of accelerating wound healing and inhibiting scar formation, and its mechanism was closely related to hemostatic function, regulation of inflammatory factors, collagen deposition, collagen fiber remodeling and intervening TGF-β/Smads signal pathway. Therefore, SNE may have promising clinical applications in skin wound repair and scar inhibition.
ObjectiveTo observe and analyze the efficacy and adverse reactions of Lacosamide (LCM) in the treatment of refractory epilepsy in children and adolescents. MethodsA retrospective cohort study was conducted on 85 patients with refractory epilepsy, with 50 males and 35 females, aged 0.5 ~ 15 years with an average age of (6.90±3.61) years, who were treated in the Department of Neurology of Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, from January 2020 to March 2023. A self-controlled study was conducted by oral LCM add on treatment, and follow-up was performed to compare and observe the efficacy as well as the adverse reactions before and after the use of LCM. ResultsBy self-control, after 12 months of follow-up after addition of LCM treatment, compared with baseline, the frequency of seizures decreased after 3, 6 and 12 months of treatment, the differences were statistically significant (P<0.05), and the effective rate of analysis after 3, 6 and 12 months of addition of treatment were 36.47%, 42.35% and 41.18%, respectively. There were 22 cases without seizure after 12 months of LCM treatment, and the seizure-free rate was 25.88%. Enrolled patients used a variety of antiseizure medications at baseline, and the three drugs used by the most patients were sodium valproate in 54 cases (63.53%), levetiracetam in 41 cases (48.24%) and oxcarbazepine in 24 cases (28.24%) respectively. After addition of LCM, a total of 10 cases experienced adverse reactions, such as dizziness, headache, nausea, etc. The incidence of adverse reactions was 11.76%. The retention rate at 12 months after adding LCM was 63.5%. ConclusionsThe addition of LCM in the treatment of refractory epilepsy in children and adolescents can effectively improve the frequency of seizures, with fewer adverse reactions and higher retention rates.
Objective To investigate the efficacy and safety of brivaracetam (BRV) as a substitute therapy for children with drug-resistant epilepsy (DRE) who have exhibited a suboptimal response to levetiracetam (LEV). MethodsTwenty-four children aged 3 to 13 years with drug-resistant epilepsy and poor response to LEV were retrospectively included. LEV was replaced with BRV within 3 days at a dose ratio of 10∶1. The maximum maintenance dose of BRV was ≤5 mg·kg?1·d?1. Patients were followed up for 6 months. Changes in seizure frequency before treatment and at 1, 3, and 6 months of treatment were observed and analyzed. Treatment-related adverse events and laboratory indicators were also recorded. ResultsThrough self-controlled analysis, after 6 months of treatment, the mean frequency of epileptic seizures in pediatric patients decreased from a baseline of 27.2±42.5 times/28 days to 8.9±14.6 times/28 days. The median frequency of seizures decreased from a baseline of 2 times/28 days to 1 time/28 days. The difference compared to baseline was statistically significant (P=0.002). The clinical response rate was 45.8%, and the complete seizure-free rate was 41.7%, with efficacy showing an increasing trend over the treatment duration. Only 3 cases (12.5%) experienced mild to moderate adverse reactions (1 case each of dizziness, nausea/vomiting, and abdominal pain). Only 1 case discontinued medication due to adverse reactions. Hematology, urinalysis, liver and kidney function, etc., showed no abnormalities, and there were no serious adverse events. ConclusionsSubstituting LEV with BRV demonstrates significant efficacy in children with DRE who have a suboptimal response to LEV. The regimen is associated with few adverse events and good tolerability, suggesting it is a viable and effective alternative clinical strategy.