Objective To investigate the cognitive attitudes of evidence-based medicine (EBM) and barriers to applying EBM in clinical practice in medical postgraduates. Methods One thousand and one hundred and thirtyseven medical postgraduates selecting the EBM courses were surveyed by questionnaires.SPSS11.5 software was used for statistical analysis. Results Medical postgraduates acquired the knowledge of EBM through classroom and seminar. Medical postgraduates though that EBM could guide clinical research and improve operational lever and sever patient. More and more postgraduates will to take part in relevant training of EBM. The barriers to using EBM in clinical practice included lack of EBM knowledge, searching and accessing evidence, having influences from clinical experience and authorities, uncertainties about the effectiveness of evidence. Conclusions EBM education has transformed from dissemination and popularization stage to application stage. We should strengthen the training of clinical evidence-based practice.by evidence-based medical education.