ObjectiveTo research on the efficient and scientific management for clean operation room. MethodsWe invited a specialist team to deal with the problems of clean operation rooms in our hospital found by a third-party monitoring organization in June 2012. Through making regulations, installing equipment, strengthening management and distributing responsibilities, we tried to correct such problems as unstandardized number of dust particles, pressure, temperature and humidity. ResultsWithin two months of reforming, under multi-disciplinary cooperation, environmental monitoring of the clean operation room reached the management standard. ConclusionStrengthening the management of operation staff, logistics, environment and equipment can assure that the clean operation room conforms to the national management standard. This can effectively prevent hospital infection and assure medical safety. Multi-disciplinary cooperation plays a very important role through the process.