Objective To examine the expression of proliferating cell nuclear antigen (PCNA) of retinal pigment epithelial (RPE) cells, thus assessing the role of mechanism of contact inhibition playing in the process of experimental retinal detachment and reattachemnt.Methods Retinal detachment was produced in 72 cats by subretinal injection of 0.25% solution of healon through a micropipette three weeks after extracapsular lens extraction and vitrectomy. Some of the detached retinae were reattached 24 hours later. At different time, the cats were killed and eye globes were fixed and embeded in paraffin. Histologic sections were processed for immunohistochemistry examination using an antibody to detect PCNA protein. Labeled RPE cells were identified, and the proliferation was quantified in detached and un-detached retinae of detachment group, and also in reattached retinae of reattachment group. The comparsion of PCNA-labeled RPE cells in different groups were analyzed by ANOVA. Results In detached regions of detachment group, PCNA-expression of RPE cells occured within 24 hours, and reached a maximum after 5-6 days, then gradually declined to barely detectable levels after 20 days. Similar tendency was found in reattached retinae, but the number of PCNA-labeled RPE cells was obviously small. Fewer PCNA-labeled RPE cells were found in regions of un-detached retinae in detachment group. The difference of these three groups was significant.Conclusion Proliferation of RPE cells is induced when they lose contact with neural retina, but inhibited after neural retina reattached to RPE cells. It suggests that the mechanism of contact inhibition plays a role in the proliferative process after retinal detachment and reattachment. (Chin J Ocul Fundus Dis，2003，19：20-23)
目的 總結腹部閉合性損傷的診治體會。方法 回顧性分析我院78例腹部閉合性損傷患者的臨床資料。結果 78例中67例手術治療，4例行腎動脈栓塞術，7例保守治療； 除1例死亡外，余均治愈。結論 及時診斷和治療是救治腹部閉合性損傷患者的關鍵，腹腔穿刺、B超、CT及X線檢查的合理應用對診斷有重要價值。
Six patients with moderate to advanced primary carcinoma of the liver were treated in this hospital with perfusion chemotherapy and embolization through the regional portal vein under the guidance of B-ultrasongraph rather than (with) operatie catheteization of the portal vein. The results show that all the tumor masses were reduced in size after the treatment (1.2-3.2cm, average value 1.9cm). It might be a new way for treating the primary carcinom of liver. The detailed procedure is descibed and the effects are also discussed in this article.
目的 總結分析普通B超監測引導PTCD方法改進后的優點及經驗。方法 用普通B型超聲診斷儀，腹部扇掃探頭和國產配套的專用PTCD套針及引流管，改進監測引導PTCD的方法，總結分析其方法的優越性。結果 95例梗阻性黃疸患者PTCD成功率為100%，引流效果良好，并發癥發生率為零。結論 用改進普通B超監測引導的方法進行PTCD，能克服在X線下進行PTCD的盲目性，對碘過敏者無禁忌，避免了長時間X線對人體的傷害。同時具有定位準確、費時少、成功率高、并發癥少、價格便宜、適宜在基層醫院推廣應用等優點。
ObjectiveTo evaluate the diagnostic value of BRAFV600E mutation test in high-risk thyroid nodules with easily underdiagnosed fine-needle aspiration biopsy (FNAB) results.MethodsRetrospectively collected 122 cases of thyroid nodule who treated in the Hebei Petrochina Central Hospital between January 2017 and December 2018, all the cases admitted preoperative ultrasound and FNAB detection. All of the patients had the non-positive cytological results of FNAB and the high-risk features of ultrasound. Contrasted the postoperative pathological coincidence rate of combination of FNAB and BRAFV600E test with FNAB alone.ResultsThe BRAFV600E mutation rate was 27.0% (33/122). The positive rate of BRAFV600E mutation increased with the increase of ultrasound thyroid imaging reporting and data system(TI-RADS) grade (P<0.05), which was independent of patients’ age, gender, number of nodules, diameter of nodules, and FNAB results (P>0.05). The coincidence rate of FNAB combined with BRAFV600E mutation detection was higher than that of FNAB alone ［86.9% (106/122) vs. 69.7% (85/122), P<0.05).ConclusionsThe BRAFV600E mutation test can detect papillary thyroid carcinoma that might be missed by FNAB. We recommend that FNAB should be routinely accompanied by the BRAFV600E mutation test in the high-risk thyroid nodules.
ObjectiveTo evaluate the effectiveness of Curved Diffusion Needle in unilateral percutaneous vertebroplasty (PVP) by compared with bilateral PVP. MethodsA clinical data of 93 patients with osteoporotic vertebral compression fracture (OVCF) treated with PVP between January 2020 and January 2021 was retrospectively analyzed, including 47 patients underwent unilateral PVP assisted with Curved Diffusion Needle (unilateral group) and 46 patients underwent bilateral PVP (bilateral group). There was no significant difference in gender, age, cause of injury, time from injury to operation, T value of bone mineral density, AO classification, distribution of injured vertebrae, and preoperative visual analogue scale (VAS) score, Oswestry disability index (ODI), relative height of injured vertebrae, and Cobb angle between the two groups (P>0.05). The operation time, the amount of bone cement injection, the incidence of bone cement leakage, the bone cement diffusion distribution, VAS score, ODI, the relative height of injured vertebrae, and Cobb angle were recorded and compared between the two groups. Results All operations successfully completed. The operation time was significantly shorter in unilateral group than in bilateral group (t=?13.936, P=0.000), and the amount of bone cement injection was significantly less in unilateral group than in bilateral group (t=?13.237, P=0.000). The incidence of bone cement leakage in unilateral group was 19.14%, which was significantly lower than that in bilateral group (39.13%) (χ2=4.505, P=0.034). The score of bone cement distribution in unilateral group was 7.0±1.3, of which 41 cases were excellent and 6 cases were well. The score of bilateral group was 7.4±0.8, of which 43 cases were excellent and 3 cases were well. There was no significant difference in score and grading of bone cement distribution between the two groups (t=?1.630, P=0.107; Z=?1.013, P=0.311). All patients were followed up and the follow-up time was 3-10 months (mean, 6.5 months) in unilateral group and 3-10 months (mean, 6.1 months) in bilateral group. The VAS score, ODI, the relative height of injured vertebrae, and Cobb angle at 24 hours after operation and last follow-up were significantly better than those before operation in the two groups (P<0.05). There were significant differences in all indicators between 24 hours after operation and last follow-up (P<0.05). There was no significant difference in all indexes between the two groups (P>0.05) at the same time point after operation. During follow-up, there was no complication such as contralateral vertebral collapse, refracture, adjacent vertebral fracture, or local kyphosis in the two groups. ConclusionUnilateral PVP assisted with Curved Diffusion Needle for OVCF is beneficial to the distribution of bone cement, which can not only achieve similar effectiveness to bilateral PVP, but also achieve shorter operation time, less bone cement injection, and lower risk of bone cement leakage.
目的 討論多窗技術+凝血酶封閉在CT導向下經皮肺穿刺活檢中的應用價值。 方法 2009年6月－2010年3月收集分析由同一工作組連續完成的CT導向下肺穿刺活檢患者共128例，其中A組58例，采用雙窗技術+注射生理鹽水；B組70例，采用多窗技術+注射凝血酶）。比較兩組患者的診斷陽性率、氣胸及肺出血發生率的差異。 結果 128例均穿刺成功，A組的穿刺診斷陽性率、氣胸發生率及肺出血發生率分別為87.9%、13.8%、17.5%。B組的穿刺診斷陽性率為92.9%，氣胸發生率為8.6%，肺出血發生率為5.7%。兩組穿刺診斷陽性率和氣胸發生率的差異無統計學意義（P＞0.05）；B組肺出血的發生率均明顯低于A組，兩組間肺內出血的發生率差異有統計學意義（P＜0.05）。 結論 多窗技術+凝血酶針道封閉技術能有助于減少氣胸、肺出血等肺穿刺活術的并發癥，具有重要的臨床應用價值。
The authors analysed the medical records of 30 patients with congenital cystic disease of the liver treated in this hospital and with a review of the article some problems of diagnosis and treatment are discussed. B-altrasonic scaner (B-US), computerized tomographic scanning and magnetic resonance imaging appeared to be most helpful in diagnosing and treating this disease. After comparing different treatments, such as aspiration .alcohol sclerotherapy, fenetration, cyst resection and partial hepatectomy, the arthors state the best results could be achieved by alcohol sclerotherapy under B-US guidence (6 cases) or fenestration (15 cases) with no postoperative complication. Malignant change was found in one patient of this group.
目的探討腹腔鏡手術中穿刺相關出血的常見原因及相應的對策。 方法對2000年5月至2010年10月期間我院腹腔鏡手術中發生與穿刺相關出血的16例患者的資料進行回顧性分析。 結果16例患者中腹壁穿刺孔腹膜層出血7例，肌層出血5例，誤傷大網膜血管3例，誤傷后腹膜血管1例，均在術中及時發現。誤傷大網膜血管病例予鏡下止血，誤傷后腹膜血管病例及時中轉開腹手術，腹膜層及肌層出血病例分別給予電灼或縫扎止血。 患者均康復出院。 結論直視進腹、規范操作和抽吸滴水試驗是避免腹腔鏡手術穿刺時血管誤傷及出血遺漏的有效方法。