目的 探討手術切除聯合液氮冷凍治療結膜鱗狀細胞癌的臨床效果。 方法 分析1998年-2008年收治的21例眼瞼結膜鱗癌患者的臨床資料。對腫瘤局限在眼球結膜和部分穹窿結膜受侵犯者行單純手術切除聯合液氮冷凍治療；部分或全部結膜及眼瞼受侵犯者行局部腫瘤切除聯合眼瞼重建及液氮冷凍治療；眼瞼和球結膜廣泛受累，無法進行眼瞼重建者行眼眶內容物剜除術。 結果 21例中，7例行單純腫瘤切除合并冷凍治療，其中5例痊愈，2例復發；10例行局部腫瘤切除并進行眼瞼重建，其中7例痊愈，3例復發，復發病例作眶內容摘除；4例腫瘤侵犯范圍較寬而無法保留眼球者行眼眶內容物摘除。術手3例死于肝肺轉移。 結論 眼瞼結膜鱗狀細胞瘤的外科手術切除是唯一有效的方法，眼瞼重建視腫瘤侵犯范圍而定，冷凍治療作為輔助治療可提高疾病的治愈率。
In the present study, the performance of the liquid nitrogen frozen and thinned bovine pericardium was studied and compared with the porcine pericardium. The microstructure and mechanical properties of the bovine pericardium were observed and tested by hematoxylin-eosin (HE) staining and tensile test respectively. In all conditions, porcine pericardium was selected as a control group. The results showed that there was little difference in the performance of bovine pericardium after being frozen by liquid nitrogen. The secant modulus and ultimate strength of the thinned bovine pericardium were similar to those of porcine pericardium, however, the elastic modulus was a little higher than porcine pericardium. The study suggested that the performance of the thinned bovine pericardium was similar to those of porcine pericardium. It was easy for the thinned bovine pericardium to obtain a relatively ideal thickness and expected performance, therefore, the thinned bovine pericardium can be used as the materials of transcatheter aortic valve leaflets.
Abstract: Objective To examine the cell viability and hemodynamic functions of the stented homograft valves preserved in liquid nitrogen. Methods Cell viability of the stented homograft valve preserved in liquid nitrogen after 3 months of preservation (experimental group，n=6) was examined using flow cytometer. Fresh homografts served as control group (n=6). We prepared three sorts of stented homograft valve(21#, 23#, 25#) preserved by liquid nitrogen. In vitro pulsatile flow tests were performed on valves of two groups. Effective opening area EOA),transvalve pressure gradient and regurgitation ratio were recorded at various flow volume, and compare with Perfect bioprosthetic valve. Results The results revealed that the death ratio of endothelial cell was 10.24%±1.71% in the experimental group, and 9.09%±2.72% in the control group (P=0.441). The death ratio of smooth muscle cell was 8.76%±1.82% in the experimental group, and 7.84%±0.59% (P=0.178) in the control group. The death ratio of total cell was 8.79%±1.44% in the experimental group, and 7.40%±0.49% in the control group (P=0.072). There were no significantly differences between two groups. The transvalve pressure gradient of two groups of valve depended on the flow volume, and increased with the flow volume increasing. The transvalve pressure gradient of the stented homograft valve was higher than that of Perfect valve. Regurgitation ratio of the stented homograft valve was bigger than Perfect valve’s. EOA had an increasing character when flow volume increased. EOA of the stented homograft valve was smaller than that of Perfect valve’s. Conclusion Liquid nitrogen can offer the benefit of cell viability of the stented homograft bioprosthetic valves. The stented homograft valve has salisfactory hemodynamic functions.