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RESEARCH PROGRESS OF MYOCARDIAL TISSUE ENGINEERING SCAFFOLD MATERIALS

Objective To review the current status and problems in the developing scaffolds for the myocardial tissue engineering appl ication. Methods The l iterature concerning the myocardial tissue engineering scaffold in recent years was reviewed extensively and summarized. Results As one of three elements for tissue engineering, a proper scafold is veryimportant for the prol iferation and differentiation of the seeding cells. The naturally derived and synthetic extracellular matrix (ECM) materials aim to closely resemble the in vivo microenvironment by acting as an active component of the developing tissue construct in myocardial tissue engineering. With the advent and continuous refinement of cell removal techniques, a new class of native ECM has emerged with some striking advantages. Conclusion Through using the principle of composite scaffold, computers and other high-technology nano-polymer technology, surface modification of traditional biological materials in myocardial tissue engineering are expected to provide ideal myocardial scaffolds.

ROLE OF STEM CELL NICHES IN MAINTAINING CARDIAC STEM CELLS HOMEOSTASIS

ObjectiveTo review the role of stem cell niches in maintaining cardiac stem cells homeostasis, and to foresee its prospects. MethodsThe literature on cardiac stem cells niches was extensively reviewed. The roles of stem cell niches components, extracellular matrix, and secretory factors in maintaining cardiac stem cell homeostasis were analysed and reviewed. ResultsLots of experiments reveal that stem cell niches are able to delay the aging of cardiac stem cells, protect from external damage, keep stem properties, and improve the purity and quantity. However, the mechanism is not fully understood. ConclusionThe stem cell niches have a very bright application prospect in homeostasis, purification, and amplification for the cardiac stem cells, and it needs further study.

肺曲菌病八例

A NEW METHOD OF ESTABLISHING EXPERIMENTAL MODEL OF CHRONIC MYOCARDIALINFARCTION BY INCISION BELOW XIPHOIDPROCESS IN BLACK GOATS

Objective To establish an effective model of myocardial infarction in black goat so as to provide a safe, convenient and credible model of myocardial infarction for treatment and research. Methods Sixteen black goats were made chronic myocardial infarction by ligation of far end of left anterior descending coronary artery through incision below xiphoidprocess. Electrocardiogram(ECG) and serum myocardial enzymes were investigated before and after occlusion. Echocardiographic measurements were performed, and left coronary artery angiography was performed with digital subtraction angiography (DSA) before infarction and 6 weeks after infarction. The myocardial ultrastructure were observed. Results All goats survived more than 6 weeks. ECG showed ambulatory change, ST-segment elevated half an hour after occlusion and pathologic Q waves 6 weeks after infarction, CK-MB significantly increased. Echocardiographic indexes showed significant decrease of maximal peak A, percent wall thickening(WHT) and ejecting fraction (EF), increase ofend-systolic volume (ESV), end-diastolic volume (EDV), and dilation of left ventricle. DSA showed block or decrease of perfusion of far end of left anterior descending coronary artery. Conclusion It is safe, convenient and credible to establish model of myocardial infarction by ligation of far end of left anterior descending coronary artery through incision below xiphoidprocess in black goat.

食管結核誤診為食管癌一例

22例心臟腫瘤的診斷與治療

Study on Downstream Flow Field Uniformity of Aortic Stenosis by Using Pulse Wave Doppler Echocardiography

ObjectiveBy comparing the difference between different stenosed degree of aortic valve in flow field uniformity and turbulent shear stress (TSS), to explore the relation between flow field uniformity and different stenosed degree of aortic valve, and probe the clinical value for deciding the operation timing, and analyze the possible role of TSS in the progress of the disease.MethodsThe flow field uniformity values and TSS in parasternal long axis plane and apical five cavity plane on each point were measured and calculated by pulse wave Doppler echocardiography technique for 33 patients with different stenosed degree of aortic valve.ResultsThere were significant difference between the different stenosed degree of aortic valve in maximal velocity difference(ΔV max )and TSS( P lt;0.05, 0.01). The more severe the aortic stenosis was, the worse the flow field uniformity was, the lower the TSS was.ConclusionsThere are significant difference between the patients of different stenosed degree of aortic valve in flow field uniformity. Flow field uniformity has important value in classifying the degree of aortic stenosis and deciding the timing of operation. The more severe the aortic stenosis is ,the lower TSS is. It can be thought that low TSS distribution has important role in pathological process of constriction in cardiovascular system diseases.

肺結核球103例

心臟刀刺傷15例

小腸黏膜下層-骨髓間充質干細胞移植-治療陳舊性心肌梗死

摘要: 目的 探討自體骨髓間充質干細胞(MSCs)小腸黏膜下層(SIS)支架復合物移植于陳舊性心肌梗死區域后細胞的存活、支架的降解情況及其對心功能的影響。 方法 將16只黑山羊按隨機數字表法分為兩組，每組8只。實驗組：建立心肌梗死模型，抽取自體骨髓,經體外分離MSCs,進行培養、傳代、BrdU標記、與SIS支架復合，并于心肌梗死6周時將MSCs-SIS復合物補片移植至陳舊性心肌梗死區；對照組：建立心肌梗死模型。于移植后6周行超聲心動圖、HE染色及免疫組織化學檢測。 結果 MSCs-SIS植入心肌梗死區2周時在補片區可見大量炎性細胞浸潤；6周時淋巴細胞浸潤消失，SIS部分降解，移植區見大量移植細胞存活。MSCs-SIS植入體內后6周時，實驗組每搏輸出量(42.81±4.91 ml vs. 37.06±4.75 ml)、射血分數(59.20%±5.41% vs. 44.56%±4.23%)、室壁增厚率（54.51%±8.60% vs. 43.36%±8.91%）、舒張期E峰 (54.85±6.35 cm/s vs. 43.14±4.81 cm/s)顯著高于對照組(Plt;0.05)，左心室收縮期末容積(29.75±5.98 ml vs. 46.25±6.68 ml)、舒張期末容積(72.55±8.13 ml vs. 83.31±8.61 ml)顯著低于對照組(Plt;0.05)。 結論 SIS作為支架移植MSCs有利于移植細胞存活，對陳舊性心肌梗死后心功能有明顯的改善作用。