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Alginate sodiumretinoic acid microspheres suppress the subretinal proliferation after laser coagulation

Objective　To observe the inhibiting effects of alginate sodiumretinoic acid（AGS-RA）microspheres release system on the laser coagulationinduced subretinal proliferation.Methods　RA were dissolved by absolute alcohol,then mixed with 1.5% AGS and made into AGSRA microspheres by a microcapsule electrostatic generator. The parameter of laser injury include irradiation time (0.20 s)，spot diameter (200 mu;ｍ) and output power (420 ｍＷ).Thirty pigmented rabbits were randomly divided into 3 groups (laser injury，experimental and control group).After laser coagulation,AGSRA or blank microspheres were immediately injected into the vitrous of experimental and control rabbits respectively.The height,width and area of 6 retinal spots of laser coagulation at each timepoint were analyzed histopathologically with serial retinal sections at 1,2,3,4,and 6 weeks after laser coagulation.Results　Histopathological examination showed that there were morphological and distribution changes of retinal cells in all layers, and localized fibroblasts proliferation in the retina after laser injury. The laserinduced responses in experimental group were much milder（P＜0.01）, while the laser injury group and control group have same width（P＞0.05）and height/area of laser spots（P＞0.05）.Conclusion　AGSRA release system can alleviate the subretinal proliferate after laser injury.

The Perioperative Nursing Care of TURP

摘要:目的:探討良性前列腺增生經尿道前列腺電切術圍手術期的護理經驗。方法:回顧性分析96例良性前列腺增生患者臨床資料。結果:96例患者手術順利,圍手術期經周密的護理,療效滿意,無明顯并發癥。結論:周密的手術期護理對經尿道前列腺電切術治療老年良性前列腺增生十分重要。Abstract: Objective: To investigate the perioperative nursing care of transurethral prostatic resection (TURP). Methods: The data of 96 TURP cases were analyzed retrospectively. Results: All the operations were performed successfully, and there were no obvious complications among the patients with precise nursing care. Conclusion: It is very important for precise nursing care to the patients who underwent TURP.

STUDY OF THE EXPRESSION OF HEAT SHOCK PROTEIN 47 AND ITS CORRELATION TO COLLAGEN DEPOSITION IN PATHOLOGICAL SCAR TISSUES

Objective To study the expression of heat shock protein 47 (HSP47) and its correlation to collagen deposition in pathological scar tissues. Methods The tissues of normal skin(10 cases), hypertrophic scar(19 cases), and keloid(16 cases) were obtained. The expression ofHSP47 was detected by immunohistochemistry method. The collagen fiber content was detected by Sirius red staining and polarization microscopy method. Results Compared with normal skin tissues(Mean IOD 13 050.17±4 789.41), the expression of HSP47 in hypertrophic scar(Mean IOD -521 159.50±272994.13) and keloid tissues(Mean IOD 407 440.30±295 780.63) was significantly high(Plt;0.01). And there was a direct correlation between the expression of HSP47 and the total collagen fiber content（r＝0.386，Plt;0.05）. Conclusion The HSP47 is highly expressed in pathological scartissues and it may play an important role in the collagen deposition of pathological scar tissues.

Effects of heat-inactivated Lactobacillus gasseri TMC0356 on liver lipid metabolism in rats with metabolic syndrome and its possible mechanism

Objective To explore the effects of heat-inactivated Lactobacillus gasseri TMC0356 on liver lipid metabolism in rats with metabolic syndrome (MS) and its possible mechanism. Methods Sixty male Sprague-Dawley rats were selected. Rats were randomly divided into 5 groups, including control group, MS model group and three TMC0356 test groups (low-, medium- and high-dose groups). The rats in each group were fed with different diets for 7 days, and the liver was dissected and removed after 15 weeks. The mRNA and protein expression levels of peroxisome hyperbioactive receptor-α (PPAR-α), sterol regulatory element binding protein-1c (REBP-1c), fatty acid synthase (FAS) and carnitine lipoacyltransferase-1 (CPT-1) genes in liver were detected. Results There was no significant difference in the mRNA expression of PPAR-α, SREBP-1c or CPT-1 among the five groups (P>0.05). The mRNA expression of FAS in low-dose TMC0356 test group was lower than that in MS model group (P=0.011), medium-dose TMC0356 test group (P=0.042) and high-dose TMC0356 test group (P=0.009). There was no significant difference in the expression of FAS mRNA between other groups (P>0.05). There was no significant difference in the protein expression of PPAR-α, SREBP-1c or FAS among the five groups (P>0.05). The protein expression of CPT-1 in low-dose TMC0356 test group was higher than that in control group (P=0.033) and high-dose TMC0356 test group (P=0.043). There was no significant difference in the protein expression of CPT-1 between the other groups (P>0.05). Conclusion Heat-inactivated Lactobacillus gasseri TMC0356 may improve the symptoms of metabolic disorder in rats by suppressing appetite, improving insulin resistance, and downregulating the expression of key fat metabolism genes such as FAS and SREBP-1c.

Inhibitive effect of E2F decoy oligodeoxynucleotide on proliferation of human retinal pigment epithelial cells in vitro

Objective To investigate the inhibitive effect of E2F decoy oligodeoxynucleotides (E2F decoy ODNs) on cultured human retinal pigment epithelial (HRPE) cells.Methods E2F decoy ODNs or scramble decoy ODNs at varied concentrations were put into the HRPE cells mediated by lipofectamineTM2000. The proliferative activity of HRPE was detected by methythiazolyl-terazollium assay, and the competitive combinative activity of E2F decoy ODNs and transcription factor E2F was detected by electrophoresis mobility-shift assay. Results The proliferation of HRPE was inhibited markedly by E2F decoy ODNs at the concentration of 0.2 μmol/L (P=0.002) in a dose-dependent manner but not by scrambled decoy. The results of electrophoresis mobility-shift assay showed that the combinative activity of transcription factor E2F was abolished completely by E2F decoy ODNs. Conclusions E2F decoy ODNs may sequence-specifically inhibit the combinative activity of transcripti on factor E2F,and inhibit the proliferation of HRPE cells.(Chin J Ocul Fundus Dis，2004，20：182-185)

核增殖抗原表達基因Ki67與人肺癌臨床病理生理特征及其預后的關系

目的 研究細胞增殖狀態與肺癌臨床病理生理特征和預后的關系，探討Ki67對臨床診斷和預后判斷的價值。方法 應用免疫組織化學法檢測166例肺癌組織標本和37例肺良性病變組織中Ki67的表達水平。結果 (1)肺癌組織中Ki67指數顯著高于肺良性病變組織(P＜0.01)，而癌旁組織與良性病變組織相比差別無顯著性意義；(2)肺癌組織中Ki67表達水平增強與肺癌組織學類型、細胞分化程度有關(P＜0.05)，而與患者PTNM分期、淋巴結轉移程度、吸煙與否以及患者的性別、年齡無明顯關系；(3)Ki67高表達組肺癌患者的術后生存時間顯著低于低表達組(P＜0.01)，按PTNM分期分層后這種差別仍然存在(P＜0.05)。結論 Ki67能較好地反映肺癌細胞的增殖狀態，并對臨床診斷和預后判斷有一定價值。

增生性視網膜前膜中單核細胞趨化蛋白-1的表達

Plasmakinetic Energy Transurethral Resection of the Prostate for Benign Prostatic Hypertrophy

摘要:目的:探討經尿道前列腺等離子切除術(PKRP)治療前列腺增生癥(BPH)的療效及安全性。方法:回顧分析采用PKRP治療的BPH患者,收集患者臨床資料,隨訪12個月,并對手術前后患者國際前列腺癥狀評分、最大尿流率、生活質量評分進行比較。結果:2006年8月至2008年8月PKRP手術治療BPH患者共238例,手術時間30～159 min,平均70 min,切除腺體25～127 g,平均54 g。無電切綜合征。術后及1年后最大尿流率、國際前列腺癥狀評分、生活質量評分三項指標較術前明顯改善(Plt;0.05)。結論:PKRP是治療BPH安全有效的治療方式。Abstract: Objective: To assess the clinical efficacy and safety of plasmakinetic energy transurethral resection of the prostate (PKRP) for benign prostatic hypertrophy (BPH). Methods: The data of patients with BPH treated with PKRP were retrospectively analyzed and the International Prostate Symptoms Scales (IPSS), maximum flow rate (Qmax) and Quality of Life (QOL) of patients with 12month followup were compared before and afteroperation and postoperation. Results: A total of 238 patients with BPH were enrolled from June 2006 to June 2008. The duration of the procedure was 70. 3 min (ranged from 30 min to 159 min) and the weight of dissected tissue was 54 g (ranged from 25 g to 127 g). No transurethral resection syndrome occurred. IPSS, Qmax and QOL were improved obviously after operation (Plt;0.05). Conclusion: PKRP is effective and safe.

胸內巨淋巴結增生癥的診斷及外科治療

目的 為了提高對胸內巨淋巴結增生癥的認識,探討其診斷方法、外科治療特點. 方法 回顧11例胸內巨淋巴結增生癥的病例,對其臨床癥狀、影象學特點、手術方式、病理分類等進行分析. 結果 胸內巨淋巴結增生癥的臨床癥狀無特異性,影象學表現為縱隔、肺部或肺門陰影和胸腔積液.9例瘤體完整切除,2例有少量殘留;術后效果良好,隨訪至今無復發. 結論 胸內巨淋巴結增生癥可以發生在胸腔的任何部位,術前確診率低,但手術效果好.病理屬良性腫瘤,故術中應盡量保留正常肺組織,并避免損傷周圍臟器.

Surgical results of persistent hyperplastic primary vitreous

Objective To observe the surgical results of the patients with persistent hyperplastic primary vitreous (PHPV). Methods Clinical data of 16 eyes of 16 patients with PHPV who had undergone retina surgery were retrospectively analyzed. The patient, 7 males and 9 females, aged from 3 months to 25 years with the average of 51.9 months. 14 cases were si n gle eye pathogenesis, 2 cases were binocular pathogenesis. Of the 16 eyes, 3 had anterior PHPV, 13 had components of both anterior and posterior disease. In addition, 10 eyes with cataract, 7 eyes with posterior synechia, 5 eyes with shallo w anterior chamber, 3 eyes with bandshaped degeneration of cornea, 1 eye with corneal opacity, 2 eyes contractive retinal detachment, 1 eye with rhegmatogenous retinal detachment. The visual acuity was light sensation, hand move, counting f ingers/10cm and 002 in 1 eye respectively. 12 eyes can not meet visual inspection, the reflection of b light was not obvious.13 eyes underwent lensectomy and anterior vitrectomy, 1 eye was implanted intraocular lens, 3 eyes with retin al detachment underwent lensectomy, vitrectomy, photocoagulation, gas tamponade and scleral buckling. Follow-up ranged from 6 months to 4 years (mean 15.3 months). Results After surgery, all 16 eyes had normal intraocular pressure and anterior chamber, retinal attachment; two eyes achieved a visual acuity of 10/100 and 20/ 100; 8 patients have the reflection of b light who once didnprime;t meet visual inspection; the visual acuity in 6 patients below counting fingers. Conclusions Surgical treatment of PHPV can prevent future glaucoma and he morrhagic complications. It is useful to win functional visual acuity if it combined with amblyopia training after surgery. （Chin J Ocul Fundus Dis，2008，24：210-212）