目的 探討乙型肝炎病毒（HBV）表面抗原（HBsAg）陽性肝硬化患者血清中HBV前S1抗原(前S1抗原)、HBV e抗原（HBeAg）及HBV核酸定量檢測（HBV DNA）相關性。 方法 2008年7月－2011年5月對97例HBsAg陽性肝硬化住院患者和50份HBsAg陰性的健康體檢者血清進行前S1抗原、HBV血清標志物檢測及實時熒光定量PCR檢測HBV DNA結果進行分析。 結果 97份HBsAg陽性肝硬化患者血清中，前S1抗原、HBeAg及HBV DNA陽性率分別為53.6%（52/97）、22.7%（22/97）及61.8%（60/97）。22例HBeAg陽性血清中，前S1抗原陽性18例（81.8%）， HBV DNA陽性20例（90.9%)。75例HBeAg陰性血清中，前S1抗原陽性34例（45.3%），HBV DNA陽性40例（53.3%），兩者的前S1抗原與HBV DNA結果間都具有很好的相關性。HBV DNA含量與前S1抗原及HBeAg陽性結果顯示：HBsAg陽性的肝硬化患者血清中HBV DNA陰性率為38.1%（含量＜103 copies/mL），而陽性檢出率HBV DNA含量主要集中在103～105 copies/mL，占81.7%（49/60），HBV DNA含量＞105 copies/mL占18.3%（11/60）。 結論 HBsAg陽性的肝硬化患者血清中主要以HBV非HBeAg陽性血清學模式為主，HBV DNA陽性檢出率的含量主要集中在103～105 copies/mL。前S1抗原在HBeAg陽性血清中與其含有HBsAg病毒及HBeAg陽性患者具有很好的相關性，而在HBeAg陰性血清中存在著差異。Objective To study the correlation among Pre-S1 antigen, HBeAg and HBV DNA results in patients with HBsAg-positive liver cirrhosis. Methods We retrospectively analyzed the serum pre-S1-antigen, HBV serum markers and real-time quantitative PCR HBV DNA results in 97 patients with HBsAg-positive liver cirrhosis and 50 HBsAg-negative healthy volunteers in our hospital from July 2008 to May 2011. Results Among the 97 samples of HBsAg-positive liver cirrhosis patients’ serum, the positive rates of Pre-S1 antigen, HBeAg and HBV DNA were 53.6% (52/97), 22.7% (22/97) and 61.8% (60/97), respectively. In the 22 samples of HBeAg-positive serum, the number of positive pre-S1 antigen and HBV DNA was 18 (81.8%) and 20, respectively. In the 75 samples of negative HBeAg serum, the number of positive pre-S1 antigen and HBV DNA was 34 (45.3%) and 40 (53.3%) respectively. The pre-S1 antigen was correlated well with HBV DNA results in both the two groups. HBV DNA level, pre-S1 antigen and HBeAg-positive results showed that the serum HBV DNA negative rate of HBsAg-positive patients with cirrhosis was 38.1% (＜103 copies/mL), while the positive rate of HBV DNA level was mainly concentrated at 103~105 copies/mL, accounting for 81.7% (49/60), and HBV DNA level over 105 copies/mLaccounted for only 18.3% (11/60). Conclusions HBsAg-positive patients with cirrhosis mainly have a serum non-HBeAg-positive HBV serology pattern, and HBV DNA positive rate of the content is mainly concentrated at 103~105 copies/mL. There is a good correlation between pre-S1 antigen in HBeAg-positive serum and patients with HBsAg virus or positive HBeAg, while for Pre-S1 antigen in HBeAg-negative serum, it is quite different.
ObjectiveTo evaluate the relationship between hepatitis B virus (HBV) preS1 antigen and the virus replication index human hepatitis B e antigen (HBeAg) and liver function. MethodsA total of 310 cases of serum samples from patients with chronic HBV treated in our hospital between May and July 2012 were examined and studied. HBV preS1 antigen was detected by ELISA; HBeAg was detected by the Architect i2000 system; and alanine transaminase (ALT) was detected by kinetic method. ResultsThe detection rate of HBV preS1 antigen was higher in HBeAg positive group. HBV preS1 antigen was correlated with HBeAg which was an index of virus replication. HBV preS1 antigen was correlated with ALT levels. ConclusionThe levels of HBV preS1 antigen may reflect the replication of HBV and the reactiveness condition of chronic hepatitis.