煙霧吸入傷后大鼠肺及腎血管緊張素轉換酶表達變化的研究_《中國呼吸與危重監護雜志》

作者：

高維 ¹ ,  金發光 ¹ , 李圓園 ² , 保鵬濤 ³

1 第四軍醫大學唐都醫院呼吸科（陜西西安 710038）;;
2 解放軍253醫院急診科（內蒙古呼和浩特 010051）;;
3 第四軍醫大學西京醫院呼吸科（陜西西安 710032）;

關鍵詞：

煙霧吸入急性肺損傷血管緊張素轉換酶血管緊張素轉換酶2

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目的探討血管緊張素轉換酶( ACE) 和ACE2 在煙霧吸入傷后大鼠肺、腎的表達變化和可能的作用機制。方法 30 只健康雄性SD 大鼠隨機分為正常對照組, 傷后1、4、10 和24 h 組, 每組各6 只。致傷組予以濃煙吸入30 min, 按組別不同在相應時間點取其肺、腎組織, 分別進行肺組織濕/ 干重測定、HE染色和ACE、ACE2 免疫組織化學檢測。結果致傷組大鼠均表現出急性肺損傷癥狀, 肺濕/ 干重均顯著高于正常對照組( P lt;0. 05 或P lt;0. 01) 。ACE、ACE2 在正常肺、腎細胞膜上有表達。肺內ACE 表達在致傷后立即升高; 而肺內ACE2 在致傷后4 h 開始明顯升高。各組間腎組織ACE 及ACE2 表達無明顯差別。結論肺組織ACE、ACE2 表達的不同變化在煙霧吸入傷的發生機制中可能有重要作用。

引用本文： 高維,金發光,李圓園,保鵬濤. 煙霧吸入傷后大鼠肺及腎血管緊張素轉換酶表達變化的研究. 中國呼吸與危重監護雜志, 2009, 09(1): 63-67. doi: 復制

1.	Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol Chem, 2000, 275 :33238-33243.
2.	Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase ( ACE2 ) converts angiotensin I to angiotensin 1-9. Circ Res, 2000, 87: 523-525.
3.	Kuba K, Imai Y, Penninger JM. Angiotensin-converting enzyme 2 in lung diseases. Curr Opin Pharmacol, 2006, 6: 271-276.
4.	謝爾凡, 楊宗城, 王孰, 等. 大鼠煙霧吸入性損傷模型的制作.中國實驗動物學雜志, 1994, 4: 219-222.
5.	Imai Y, Kuba K, Penninger JM. The discovery of angiotensin-converting enzyme 2 and its role in acute lung injury in mice, Exp Physiol,2008, 93: 543-548.
6.	張維, 王導新. 肺泡上皮細胞與急性肺損傷水腫液清除的研究進展. 中國呼吸與危重監護雜志, 2008, 1: 74-77.
7.	Marshall RP, Webb S, Bellingan GJ, et al. Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med, 2002, 166: 646-650.
8.	Brizio-Molteni L, Piano G, Warpeha RL, et al. Angiotensin-1 -converting enzyme activity as index of pulmonary damage in thermal injury with or without smoke inhalation. Ann Clin Lab Sci, 1992, 22 : 1-10.
9.	Imai Y, Kuba K, Rao S, et al. Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature, 2005, 436: 112-116.
10.	Skeggs LT, Dorer FE, Lecine M, et al. The biochemistry of the reninangiotensin system. Adv Exp Med Biol, 1980, 130: 1-27.
11.	Corvol P, Williams TA, Soubrier F. Peptidyl dipeptidase A: angiotensin I-converting enzyme. Methods Enzymol, 1995, 248: 283-305.
12.	Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature, 2002 ,417: 822-828.
13.	Yamamoto K, Ohishi M, Katsuya T, et al. Deletion of angiotensinconverting enzyme 2 accelerates pressure overload-induced cardiac dysfunction by increasing local angiotensin II. Hypertension, 2006 ,47: 718-726.
14.	Imai Y, Kuba K, Penninger JM. The renin-angiotensin system in acute respiratory distress syndrome. Drug Discovery Today: Disease Mechanisms, 2006, 2: 225-229.
15.	Rivière G, Michaud A, Breton C, et al. Angiotensin-converting enzyme 2 ( ACE2) and ACE activities display tissue-specific sensitivity to undernutrition-programmed hypertension in the adult rat. Hypertension,2005, 46: 1169-1174.
16.	史家欣, 趙中蘇. 腎素-血管緊張素系統和應激在延誤吸入性急性肺損傷中的作用. 西北國防醫學雜志, 2006, 27: 214-216.
17.	Koka V, Huang XR, Chung AC, et al. Angiotensin II up-regulates angiotensin I-converting enzyme ( ACE) , but down-regulates ACE2 via the AT1-ERK/p38 MAP kinase pathway. Am J Pathol, 2008,172: 1174-1183.

1. Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol Chem, 2000, 275 :33238-33243.
2. Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase ( ACE2 ) converts angiotensin I to angiotensin 1-9. Circ Res, 2000, 87: 523-525.
3. Kuba K, Imai Y, Penninger JM. Angiotensin-converting enzyme 2 in lung diseases. Curr Opin Pharmacol, 2006, 6: 271-276.
4. 謝爾凡, 楊宗城, 王孰, 等. 大鼠煙霧吸入性損傷模型的制作.中國實驗動物學雜志, 1994, 4: 219-222.
5. Imai Y, Kuba K, Penninger JM. The discovery of angiotensin-converting enzyme 2 and its role in acute lung injury in mice, Exp Physiol,2008, 93: 543-548.
6. 張維, 王導新. 肺泡上皮細胞與急性肺損傷水腫液清除的研究進展. 中國呼吸與危重監護雜志, 2008, 1: 74-77.
7. Marshall RP, Webb S, Bellingan GJ, et al. Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med, 2002, 166: 646-650.
8. Brizio-Molteni L, Piano G, Warpeha RL, et al. Angiotensin-1 -converting enzyme activity as index of pulmonary damage in thermal injury with or without smoke inhalation. Ann Clin Lab Sci, 1992, 22 : 1-10.
9. Imai Y, Kuba K, Rao S, et al. Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature, 2005, 436: 112-116.
10. Skeggs LT, Dorer FE, Lecine M, et al. The biochemistry of the reninangiotensin system. Adv Exp Med Biol, 1980, 130: 1-27.
11. Corvol P, Williams TA, Soubrier F. Peptidyl dipeptidase A: angiotensin I-converting enzyme. Methods Enzymol, 1995, 248: 283-305.
12. Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature, 2002 ,417: 822-828.
13. Yamamoto K, Ohishi M, Katsuya T, et al. Deletion of angiotensinconverting enzyme 2 accelerates pressure overload-induced cardiac dysfunction by increasing local angiotensin II. Hypertension, 2006 ,47: 718-726.
14. Imai Y, Kuba K, Penninger JM. The renin-angiotensin system in acute respiratory distress syndrome. Drug Discovery Today: Disease Mechanisms, 2006, 2: 225-229.
15. Rivière G, Michaud A, Breton C, et al. Angiotensin-converting enzyme 2 ( ACE2) and ACE activities display tissue-specific sensitivity to undernutrition-programmed hypertension in the adult rat. Hypertension,2005, 46: 1169-1174.
16. 史家欣, 趙中蘇. 腎素-血管緊張素系統和應激在延誤吸入性急性肺損傷中的作用. 西北國防醫學雜志, 2006, 27: 214-216.
17. Koka V, Huang XR, Chung AC, et al. Angiotensin II up-regulates angiotensin I-converting enzyme ( ACE) , but down-regulates ACE2 via the AT1-ERK/p38 MAP kinase pathway. Am J Pathol, 2008,172: 1174-1183.

《中國呼吸與危重監護雜志》

煙霧吸入傷后大鼠肺及腎血管緊張素轉換酶表達變化的研究

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《中國呼吸與危重監護雜志》

煙霧吸入傷后大鼠肺及腎血管緊張素轉換酶表達變化的研究

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