依達拉奉對膿毒癥大鼠肺損傷的保護作用_《中國呼吸與危重監護雜志》

作者：

邢海波 ¹ ,  李剛 ² , 徐亞平 ³ , 高福云 ³ , 劉國玲 ³

1 紹興市人民醫院ICU（浙江紹興 312000）;;
2 衛生部中日友好醫院ICU（北京 100029）;;
3 衛生部中日友好醫院臨床醫學研究所（北京 100029）;

關鍵詞：

急性肺損傷依達拉奉核因子κB 活性氧髓過氧化物酶丙二醛超氧化物歧化酶

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目的探討自由基清除劑依達拉奉( ED) 對膿毒癥致ALI 的保護作用。方法 24 只雄性Wistar 大鼠隨機分成3 組: 對照組( NS組) 、模型組( LPS 組) 及依達拉奉治療組( ED 組) 。LPS 組和ED 組采用尾靜脈注射LPS( 10 mg/ kg) 建立ALI 模型, ED 組隨后立即尾靜脈注射依達拉奉( 3 mg/kg) 。LPS 注射6 h后留取肺標本, 分別測定肺組織濕/干重比值( W/D) 和肺組織勻漿中髓過氧化物酶( MPO) 、丙二醛( MDA) 和超氧化物歧化酶( SOD) 含量, 觀察肺組織病理改變及肺組織核因子κB( NF-κB) 表達情況。結果 LPS 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達均高于NS組( P 均 lt;0. 01) , 肺組織SOD 含量低于NS 組( P lt;0. 01) 。ED 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達均低于LPS組( P 均 lt;0. 01) , 仍高于NS 組( P 均 lt;0. 01) ;ED 組肺組織SOD 含量高于LPS 組( P lt;0. 01) , 仍低于NS 組( P lt;0. 01) 。結論依達拉奉可以減輕LPS 誘導的大鼠ALI, 其機制可能與清除ROS, 降低了NF-кB 信號轉導通路的活化, 進而減輕炎癥級聯放大效應有關。

引用本文： 邢海波,李剛,徐亞平,高福云,劉國玲. 依達拉奉對膿毒癥大鼠肺損傷的保護作用. 中國呼吸與危重監護雜志, 2009, 09(2): 176-180. doi: 復制

1.	錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認識. 中國呼吸與危重監護雜志, 2002, 1: 199-201.
2.	Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106.
3.	Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130.
4.	Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229.
5.	Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317.
6.	Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311.
7.	:Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458.
8.	Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755.
9.	林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達及其臨床意義. 中國呼吸與危重監護雜志, 2007, 6: 205-207, 229.
10.	Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59.
11.	Atabai K, Matthay MA. The pulmonary physician in critical care.
12.	Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235.
13.	Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63.
14.	Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
15.	Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156.
16.	Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352.
17.	Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104.
18.	Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431.
19.	Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
20.	Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58.
21.	Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367.

1. 錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認識. 中國呼吸與危重監護雜志, 2002, 1: 199-201.
2. Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106.
3. Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130.
4. Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229.
5. Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317.
6. Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311.
7. :Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458.
8. Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755.
9. 林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達及其臨床意義. 中國呼吸與危重監護雜志, 2007, 6: 205-207, 229.
10. Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59.
11. Atabai K, Matthay MA. The pulmonary physician in critical care.
12. Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235.
13. Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63.
14. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
15. Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156.
16. Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352.
17. Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104.
18. Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431.
19. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
20. Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58.
21. Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367.

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《中國呼吸與危重監護雜志》

依達拉奉對膿毒癥大鼠肺損傷的保護作用

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