Analysis of iron regulated locus in <i>Klebsiella Pneumoniae</i> isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital _Chinese Journal of Evidence-Based Medicine

Authors：

ZHANG Jie ,  YU Hua

Department of Clinical Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, 610072, P.R.China;

Corresponding?author：

YU Hua, Email: yvhua2002@163.com

Keywords：

Klebsiella pneumoniae; Iron regulated locus; Liver abscess; Risk factor

DOI：

10.7507/1672-2531.201708029

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Objective To investigate the iron regulated locus in Klebsiella pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital. Methods From January to December of 2015, a total of 10 isolates of Klebsiella pneumoniae were collected from blood culture of liver abscess patients from Sichuan Provincial People’s Hospital. The genomic DNA was extracted to identify the genes of iroB, iroC, and iroD by PCR, and data was further analyzed by Graphpad Prism 5 software. Results Among the 10 Klebsiella pneumoniae clinical strains, 9 strains were iroB positive strains, 9 strains were iroC positive strains, and 10 strains were iroD positive strains, 9 strains were iroB/C/D triple positive. Conclusion The current study suggests that the frequency of triple positive of iroB/C/D in Klebsiella pneumoniae is high in isolates from liver abscess patients, the triple positive of iroB/C/D may contribute to liver abscess.

Citation： ZHANG Jie, YU Hua. Analysis of iron regulated locus in Klebsiella Pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital . Chinese Journal of Evidence-Based Medicine, 2017, 17(8): 907-909. doi: 10.7507/1672-2531.201708029 Copy

1.	Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
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3.	Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
4.	Sorsa LJ, Dufke S, Heesemann J, et al. Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor. Infect Immun, 2003, 71(6): 3285-3293.
5.	B?umler AJ, Norris TL, Lasco T, et al. IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J Bacteriol, 1998, 180(6): 1446-1453.
6.	Caza M, Lépine F, Milot S, et al. Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelins. Infect Immun, 2008, 76(8): 3539-3549.
7.	Porcheron G, Garénaux A, Proulx J, et al. Iron, copper, zinc, and manganese transport and regulation in pathogenic enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence. Front Cell Infect Microbiol, 2013, 3: 90.
8.	Holden VI, Bachman MA. Diverging roles of bacterial siderophores during infection. Metallomics, 2015, 7(6): 986-995.
9.	Hsieh PF, Lin TL, Lee CZ, et al. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis, 2008, 197(12): 1717-1727.
10.	Hsu CR, Lin TL, Chen YC, et al. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology, 2011, 157(Pt 12): 3446-3457.
11.	Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.
12.	吳華, 李東冬, 王京, 等. 肺炎克雷伯桿菌肝膿腫的臨床及微生物特征分析. 中華醫學雜志, 2015, 95(40): 3259-3263.
13.	陳祥, 劉靜, 高崧, 等. 禽病原性大腸桿菌 iro 和 tsh 基因缺失株的構建. 生物工程學報, 2008, 24(3): 401-408.
14.	Schmidt PJ. Regulation of iron metabolism by hepcidin under conditions of inflammation. J Biol Chem, 2015, 290(31): 18975-18983.
15.	劉強, 曹鋒, 王亞軍, 等. 細菌性肝膿腫經皮穿刺置管引流與細針抽吸療效比較. 中國普外基礎與臨床雜志, 2010, 17(10): 1083-1086.

1. Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
2. Nairz M, Haschka D, Demetz E, et al. Iron at the interface of immunity and infection. Front Pharmacol, 2014, 5: 152.
3. Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
4. Sorsa LJ, Dufke S, Heesemann J, et al. Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor. Infect Immun, 2003, 71(6): 3285-3293.
5. B?umler AJ, Norris TL, Lasco T, et al. IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J Bacteriol, 1998, 180(6): 1446-1453.
6. Caza M, Lépine F, Milot S, et al. Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelins. Infect Immun, 2008, 76(8): 3539-3549.
7. Porcheron G, Garénaux A, Proulx J, et al. Iron, copper, zinc, and manganese transport and regulation in pathogenic enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence. Front Cell Infect Microbiol, 2013, 3: 90.
8. Holden VI, Bachman MA. Diverging roles of bacterial siderophores during infection. Metallomics, 2015, 7(6): 986-995.
9. Hsieh PF, Lin TL, Lee CZ, et al. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis, 2008, 197(12): 1717-1727.
10. Hsu CR, Lin TL, Chen YC, et al. The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology, 2011, 157(Pt 12): 3446-3457.
11. Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.
12. 吳華, 李東冬, 王京, 等. 肺炎克雷伯桿菌肝膿腫的臨床及微生物特征分析. 中華醫學雜志, 2015, 95(40): 3259-3263.
13. 陳祥, 劉靜, 高崧, 等. 禽病原性大腸桿菌 iro 和 tsh 基因缺失株的構建. 生物工程學報, 2008, 24(3): 401-408.
14. Schmidt PJ. Regulation of iron metabolism by hepcidin under conditions of inflammation. J Biol Chem, 2015, 290(31): 18975-18983.
15. 劉強, 曹鋒, 王亞軍, 等. 細菌性肝膿腫經皮穿刺置管引流與細針抽吸療效比較. 中國普外基礎與臨床雜志, 2010, 17(10): 1083-1086.

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Analysis of iron regulated locus in Klebsiella Pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People’s Hospital

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