Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice <i>In Vivo</i>_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YUANQing ¹ ,  LIBo ² , JIANGShi-ping ¹ , LUOLan-lun ³ , LIGeng ¹ , ZHAOQiang ¹ , ZHANGLi-yong ¹

1. Department of General Surgery, People's Hospital of Yaan City, Yaan 625000, Sichuan Province, China;
2. Department of Hepatobiliary Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China;
3. Department of Hepatobiliary Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China;

Corresponding?author：

LIBo, Email: liboer2002@126.com

Keywords：

Gamma ray; Echinococcosis granulosus protoscoleces; Radiotherapy; Inhibition of growth

DOI：

10.7507/1007-9424.20150039

Video：

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Objective To investigate the effect ofγ-ray irradiation on echinococcus protoscoleces before abdominal cavity implantation in mice. Methods Echinococcus protoscoleces were cultured in vitro, and then divided into 5 groups, four groups receivedγ-ray irradiation with 10 Gy, 20 Gy, 40 Gy, and 80 Gy, respectively. The remaining control group was not irradiated. The protoscoleces were then implanted into the abdominal cavity of mice. Four months later, the incidence of echinococcosis was measured by detection of echinococcus antibody in the blood and then abdominal laparotomy was performed to isolate intraabdominal cysts, and the weight and structure of the echinococcus generated from the protoscoleces were measured and observed. Results After implantation of irradiated echinococcus protoscoleces in mice, the incidence of echinococcosis was 100% in the control group, 80.0% in the 10 Gy group, 33.3% in the 20 Gy group, 33.3% in the 40 Gy group, and 26.7% in the 80 Gy group. The echinococci had lower weight in the irradiated groups with 10 Gy (35.80 mg), with 20 Gy (0.00 mg), with 40 Gy (0.00 mg), and with 80 Gy (0.00 mg) as compared with the control group (157.80 mg). The echinococcus generated from the protoscoleces appeared calcification. Conclusions γ-ray irradiation could inhibit the formation of echinococcus granulosus generated from protoscoleces, damage the structure of echinococcus generated from the protoscoleces. Irradiation might prevent echinococcosis recurrence after surgical removal of hydatid cysts.

Citation： YUANQing, LIBo, JIANGShi-ping, LUOLan-lun, LIGeng, ZHAOQiang, ZHANGLi-yong. Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(2): 139-143. doi: 10.7507/1007-9424.20150039 Copy

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8.	Uhl W, Loftier H, Zimmer MA, et al. Surgical treatment of echinococcosis of the liver. Swiss Surg, 1999, 5(1): 126-132.
9.	袁慶, 李波, 賀凱, 等.伽馬射線體外照射對細粒棘球蚴原頭節的殺滅作用.中國人獸共患病學報, 2013, 29(9): 904-908.
10.	趙春芳, 溫浩, 王建華, 等. 3種藥物體外抗細粒棘球蚴原頭節作用的比較.中國寄生蟲病防治雜志, 2002, 15(1): 35-38.
11.	袁麗英, 張壯志, 石保新, 等.細粒棘球蚴-原頭蚴體外培養成囊模型的建立.畜牧與獸醫, 2009, 41(7): 29-31.
12.	溫浩, 丁兆勛.包蟲病圖譜第二版.北京:科學出版社, 2008: 28-29.
13.	曹得萍, 馬祁生, 趙海龍.藏藥治療小鼠繼發性棘球蚴超微結構的觀察.中國人獸共患病學報, 2006, 22(7): 654-656.
14.	Smyth TD, Heath DD. Pathogeresis of larval cestodes in mammals. Helminthol Abs, 1970, 39: 1-23.
15.	張積仁, 劉端祺.腫瘤物理治療新技術.北京:人民軍醫出版社, 2005: 224-225.

1. ?包根書, 張虹, 景濤, 等.阿苯達唑亞砜及其對映體體外抗細粒棘球絳蟲原頭蚴作用.中國寄生蟲學與寄生蟲病雜志, 2008, 26(6): 459-461, 465.
2. 康金鳳, 胡漢華, 白山別克, 等.過氧化氫體外誘導細粒棘球蚴原頭節細胞凋亡的實驗觀察.中國寄生蟲學與寄生蟲病雜志, 2008, 26(5): 332-337.
3. 呂海龍, 彭心宇, 張士杰, 等.阿苯達唑脂質體及槐耳浸膏對小鼠肝細粒棘球蚴感染率影響的實驗觀察.中國寄生蟲學與寄生蟲病雜志, 2008, 26(5): 361-365.
4. Adas G, Arikan S, Kemik O, et al. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol, 2009, 15(1): 112-116.
5. 蔣松, 賈斌, 陳玲香, 等.阿苯達唑及其脂質體治療羊肝包蟲病的病理學比較觀察.黑龍江畜牧獸醫:科技版, 2009, 10(10): 82-83.
6. 呂西, 李徐生.腹腔鏡治療肝包蟲的現狀與展望.中華腔鏡外科雜志:電子版, 2010, 3(1): 75-79.
7. Gollackner B, L?ngle F, Auer H, et al. Radical surgical therapy of abdominal cystic hydatid disease: factors of recurrence. World J Surg, 2000, 24(6): 717-721.
8. Uhl W, Loftier H, Zimmer MA, et al. Surgical treatment of echinococcosis of the liver. Swiss Surg, 1999, 5(1): 126-132.
9. 袁慶, 李波, 賀凱, 等.伽馬射線體外照射對細粒棘球蚴原頭節的殺滅作用.中國人獸共患病學報, 2013, 29(9): 904-908.
10. 趙春芳, 溫浩, 王建華, 等. 3種藥物體外抗細粒棘球蚴原頭節作用的比較.中國寄生蟲病防治雜志, 2002, 15(1): 35-38.
11. 袁麗英, 張壯志, 石保新, 等.細粒棘球蚴-原頭蚴體外培養成囊模型的建立.畜牧與獸醫, 2009, 41(7): 29-31.
12. 溫浩, 丁兆勛.包蟲病圖譜第二版.北京:科學出版社, 2008: 28-29.
13. 曹得萍, 馬祁生, 趙海龍.藏藥治療小鼠繼發性棘球蚴超微結構的觀察.中國人獸共患病學報, 2006, 22(7): 654-656.
14. Smyth TD, Heath DD. Pathogeresis of larval cestodes in mammals. Helminthol Abs, 1970, 39: 1-23.
15. 張積仁, 劉端祺.腫瘤物理治療新技術.北京:人民軍醫出版社, 2005: 224-225.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo

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