Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β<sub>1</sub> and basic fibroblast growth factor induced membrane by Masquelet technology_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DING Qiang , SUN Nan ,  WANG Xiaohui

Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, P.R.China;

Corresponding?author：

WANG Xiaohui, Email: 963099784@qq.com

Keywords：

Bone defect; glycoside of short-horned epimedium Herb; Masquelet technique; osteogenic activity; transforming growth factor β₁; basic fibroblast growth factor

DOI：

10.7507/1002-1892.201905060

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Objective To observe the expressions of transforming growth factor β₁ (TGF-β₁) and basic fibroblast growth factor (bFGF) induced membrane by Masquelet technique in rats treated with glycoside of short-horned epimedium Herb, and to explore the effect of glycoside of short-horned epimedium Herb on Masquelet induced membrane.Methods Sixty 3-month-old male Wistar rats were randomly divided into 3 groups with 20 rats in each group; a tibial bone defect (6 mm in length) model was established. The blank group (group A) was not treated; the control group (group B) and the experimental group (group C) were filled with vancomycin antibiotic bone cement in the drawing stage, and the bone cement was completely solidified. Group C was given perfused flavonoids glycoside of short-horned epimedium Herb (10 μmol/L) by gavage once a day (0.3 mL) from 1 day after operation, and groups A and B were given the same amount of normal saline by gavage. After operation, the recovery and wound healing of experimental animals were observed; at 4 weeks after operation, X-ray film was taken to observe the recovery of bone defect of proximal tibia; at 6 weeks after operation, the bone defect was observed, and the morphological changes and vascularization degree of granulation tissue and induction membrane tissue were observed; the expressions of TGF-β₁ and bFGF were observed by immunohistochemistry staining and ELISA detection.Results The bone defect models of the 3 groups were established successfully, and there was no abnormality after operation. The incisions healed by first intention after operation. At 4 weeks after operation, X-ray films of proximal tibial defect showed that there was obvious space in group A, while bone cement was filled and Kirschner wire fixation was good in groups B and C. At 6 weeks after operation, the gross observation showed that the granulation tissue was filled in the defect area in group A; transparent membrane was formed in groups B and C, and microvessels were seen in some areas, and the microvessels in group C were significantly more than those in group B. Immunohistochemical staining showed that the expressions of TGF-β₁ and bFGF were negative in group A, but they were expressed in groups B and C, and the expressions of TGF-β₁ and bFGF in group B were significantly less than those in group C. ELISA detection showed that the expressions of TGF-β₁ and bFGF in group C were significantly higher than those in groups A and B (P<0.05), but there was no significant difference between groups A and B (P>0.05). Conclusion Glycoside of short-horned epimedium Herb can significantly increase the expressions of TGF-β₁ and bFGF, accelerate the process of osteogenesis, and contribute to bone shaping and reconstruction.

Citation： DING Qiang, SUN Nan, WANG Xiaohui. Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology. Chinese Journal of Reparative and Reconstructive Surgery, 2020, 34(11): 1405-1409. doi: 10.7507/1002-1892.201905060 Copy

1.	何偉, 李自力, 崔元璐, 等. 淫羊藿苷對大鼠成骨細胞核結合因子 α1、骨形成蛋白-2、骨形成蛋白-4 mRNA 表達的影響. 北京大學學報 (醫學版), 2009, 41(6): 669-673.
2.	馬秀武, 程迅生. 自體骨移植修復骨缺損的研究進展. 中國骨與關節損傷雜志, 2011, 26(6): 574-576.
3.	Wang XL, Wang NL, Zhang Y, et al. Effects of eleven flavonoids from the osteoprotective fraction of Drynaria fortunei (KUNZE) J. SM. on osteoblastic proliferation using an osteoblast-like cell line. Chem Pharm Bull (Tokyo), 2008, 56(1): 46-51.
4.	王日雄, 林建華, 修忠標, 等. 鹿茸多肽誘導自體骨髓間充質干細胞移植修復兔膝關節軟骨缺損. 中國骨與關節損傷雜志, 2007, 22(11): 916-918.
5.	高怡加, 黃培鎮, 李悅, 等. 骨碎補總黃酮對牽張成骨過程中骨形態發生蛋白-2 和轉化生長因子-β₁ 表達的影響. 廣州中醫藥大學學報, 2016, 33(5): 679-683.
6.	劉印, 賀利平, 田京. 不同時期 bFGF 或副甲狀腺激素相關肽對 TGF-β₁ 誘導的兔 BMSCs 向軟骨細胞分化的影響. 中國修復重建外科雜志, 2013, 27(2): 199-206.
7.	Gouron R, Petit L, Boudot C, et al. Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique. J Tissue Eng Regen Med, 2017, 11(2): 382-389.
8.	章元沛. 藥理學實驗. 北京: 人民衛生出版社, 1996: 28.
9.	劉劍剛, 謝雁鳴, 徐哲, 等. 骨碎補總黃酮的活血化瘀作用及對實驗性微循環障礙和骨質疏松癥的影響. 中國骨質疏松雜志, 2006, 12(1): 46-49.
10.	Apard T, Bigorre N, Cronier P, et al. Two-stage reconstruction of post-traumatic segmental tibia bone loss with nailing. Orthop Traumatol Surg Res, 2010, 96(5): 549-553.
11.	Wong RW, Rabie AB. Systemic effect of crude extract from rhizome of Drynaria fortunei on bone formation in mice. Phytother Res, 2006, 20(4): 313-315.
12.	Flamans B, Pauchot J, Petite H, et al. Use of the induced membrane technique for the treatment of bone defects in the hand or wrist, in emergency. Chir Main, 2010, 29(5): 307-314.
13.	Masquelet AC, Fitoussi F, Begue T, et al. Reconstruction of the long bones by the induced membrane and spongy autograft. Ann Chir Plast Esthet, 2000, 45(3): 346-353.
14.	高子范, 楊宗智, 馬克昌, 等. 淫羊藿注射液對試管內雞胚股骨生長的促進作用. 中西醫結合雜志, 1985, 5(3): 172-174, 133.
15.	李樹源, 周琦石, 李悅, 等. 不同組織部位誘導膜血管化及成骨因子表達的比較. 中國組織工程研究, 2020, 24(5): 667-672.
16.	蔡群斌, 李定, 李悅, 等. 骨碎補總黃酮對 Masquelet 技術誘導膜內血管形成的影響. 廣州中醫藥大學學報, 2017, 34(6): 867-871.

1. 何偉, 李自力, 崔元璐, 等. 淫羊藿苷對大鼠成骨細胞核結合因子 α1、骨形成蛋白-2、骨形成蛋白-4 mRNA 表達的影響. 北京大學學報 (醫學版), 2009, 41(6): 669-673.
2. 馬秀武, 程迅生. 自體骨移植修復骨缺損的研究進展. 中國骨與關節損傷雜志, 2011, 26(6): 574-576.
3. Wang XL, Wang NL, Zhang Y, et al. Effects of eleven flavonoids from the osteoprotective fraction of Drynaria fortunei (KUNZE) J. SM. on osteoblastic proliferation using an osteoblast-like cell line. Chem Pharm Bull (Tokyo), 2008, 56(1): 46-51.
4. 王日雄, 林建華, 修忠標, 等. 鹿茸多肽誘導自體骨髓間充質干細胞移植修復兔膝關節軟骨缺損. 中國骨與關節損傷雜志, 2007, 22(11): 916-918.
5. 高怡加, 黃培鎮, 李悅, 等. 骨碎補總黃酮對牽張成骨過程中骨形態發生蛋白-2 和轉化生長因子-β₁ 表達的影響. 廣州中醫藥大學學報, 2016, 33(5): 679-683.
6. 劉印, 賀利平, 田京. 不同時期 bFGF 或副甲狀腺激素相關肽對 TGF-β₁ 誘導的兔 BMSCs 向軟骨細胞分化的影響. 中國修復重建外科雜志, 2013, 27(2): 199-206.
7. Gouron R, Petit L, Boudot C, et al. Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique. J Tissue Eng Regen Med, 2017, 11(2): 382-389.
8. 章元沛. 藥理學實驗. 北京: 人民衛生出版社, 1996: 28.
9. 劉劍剛, 謝雁鳴, 徐哲, 等. 骨碎補總黃酮的活血化瘀作用及對實驗性微循環障礙和骨質疏松癥的影響. 中國骨質疏松雜志, 2006, 12(1): 46-49.
10. Apard T, Bigorre N, Cronier P, et al. Two-stage reconstruction of post-traumatic segmental tibia bone loss with nailing. Orthop Traumatol Surg Res, 2010, 96(5): 549-553.
11. Wong RW, Rabie AB. Systemic effect of crude extract from rhizome of Drynaria fortunei on bone formation in mice. Phytother Res, 2006, 20(4): 313-315.
12. Flamans B, Pauchot J, Petite H, et al. Use of the induced membrane technique for the treatment of bone defects in the hand or wrist, in emergency. Chir Main, 2010, 29(5): 307-314.
13. Masquelet AC, Fitoussi F, Begue T, et al. Reconstruction of the long bones by the induced membrane and spongy autograft. Ann Chir Plast Esthet, 2000, 45(3): 346-353.
14. 高子范, 楊宗智, 馬克昌, 等. 淫羊藿注射液對試管內雞胚股骨生長的促進作用. 中西醫結合雜志, 1985, 5(3): 172-174, 133.
15. 李樹源, 周琦石, 李悅, 等. 不同組織部位誘導膜血管化及成骨因子表達的比較. 中國組織工程研究, 2020, 24(5): 667-672.
16. 蔡群斌, 李定, 李悅, 等. 骨碎補總黃酮對 Masquelet 技術誘導膜內血管形成的影響. 廣州中醫藥大學學報, 2017, 34(6): 867-871.

Chinese Journal of Reparative and Reconstructive Surgery

Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology

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Chinese Journal of Reparative and Reconstructive Surgery

Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β1 and basic fibroblast growth factor induced membrane by Masquelet technology

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Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β₁ and basic fibroblast growth factor induced membrane by Masquelet technology