氫氣飽和生理鹽水對腹主動脈瘤大鼠主動脈殘余應力的影響_《中國修復重建外科雜志》

作者：

宋玉祥 ¹ , 陳峰 ¹ , 熊江 ¹ , 郭偉 ¹ , 潘秀頡 ² , 賈森皓 ¹ , 劉杰 ¹

1解放軍總醫院血管外科（北京，100853）;;
2 軍事醫學科學院放射與輻射醫學研究所;

關鍵詞：

氫氣飽和生理鹽水腹主動脈瘤張開角殘余應力血管重建大鼠

DOI：

10.7507/1002-1892.20130193

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的通過觀察腹主動脈瘤（abnormal aortic aneurysm，AAA）大鼠腹主動脈直徑、擴張率、血管壁厚度及張開角等指標的變化，了解氫氣飽和生理鹽水對AAA大鼠主動脈殘余應力的影響，探討其對AAA形成的抑制作用。方法選取健康雄性SD大鼠20只（體重200～220 g），采用腎下腹主動脈CaCl2孵育法制備大鼠AAA模型，隨機分為實驗組和對照組，每組10只。建模后當天開始，實驗組于腹腔注射氫氣飽和生理鹽水（5 mL/kg），對照組注射生理鹽水（5 mL/ kg）；每天1次，共28 d。建模后28 d處死兩組動物，測量腹主動脈直徑并計算擴張率，選取瘤體位置動脈環測量腹主動脈張開角及血管壁厚度，并對標本行HE染色和醛品紅彈力纖維特殊染色觀察主動脈壁炎性細胞浸潤及中膜彈力纖維破壞情況。結果建模后28 d，實驗組與對照組腹主動脈直徑均較術前顯著增加（P lt; 0.01）；擴張率分別為65% ± 15%和128% ± 54%，實驗組顯著低于對照組（t=3.611，P=0.005）。實驗組與對照組腹主動脈張開角分別為（88.78 ± 29.20）°和（44.23 ± 28.52）°，血管壁厚度分別為（0.14 ± 0.03）mm和（0.36 ± 0.05）mm，兩組比較差異均有統計學意義（P lt; 0.01）。組織學觀察示，實驗組主動脈壁存在少量炎性細胞浸潤，彈力纖維未見明顯受損；對照組主動脈壁炎性細胞浸潤明顯，彈力纖維斷裂受損嚴重。結論氫氣飽和生理鹽水通過增大主動脈的殘余應力、減輕血管壁的組織重建程度，來維持腹主動脈更好的力學特性，減少腹主動脈擴張，抑制AAA的形成。

引用本文： 宋玉祥,陳峰,熊江,郭偉,潘秀頡,賈森皓,劉杰. 氫氣飽和生理鹽水對腹主動脈瘤大鼠主動脈殘余應力的影響. 中國修復重建外科雜志, 2013, 27(7): 881-884. doi: 10.7507/1002-1892.20130193 復制

1.	White JJ, Dalman RL. Gaining new insights into early abdominal aortic aneurysm disease. Perm J, 2008, 12(2): 10-14.
2.	Fung YC. What are the residual stresses doing in our blood vessels? Ann Biomed Eng, 1991, 19(3): 237-249.
3.	Ohsawa I, Nishimaki K, Yamagata K, et al. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochem Biophys Res Commun, 2008, 377(4): 1195-1198.
4.	Shen L, Wang J, Liu K, et al. Hydrogen-rich saline is cerebroprotective in a rat model of deep hypothermic circulatory arrest. Neurochem Res, 2011, 36(8): 1501-1511.
5.	Kajiya M, Silva MJ, Sato K, et al. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun, 2009, 386(1): 11-15.
6.	Isenburg JC, Simionescu DT, Starcher BC, et al. Elastin stabilization for treatment of abdominal aortic aneurysms. Circulation, 2007, 115(13): 1729-1737.
7.	吳曉飛, 張健, 辛世杰, 等. 雌激素在大鼠腹主動脈瘤形成中的作用. 中華醫學雜志, 2007, 87(7): 471-474.
8.	Fung YC, Liu SQ. Changes of zero-stress state of rat pulmonary arteries in hypoxic hypertension. J Appl Physiol, 1991, 70(6): 2455-2470.
9.	Liu SQ, Fung YC. Relationship between hypertension, hypertrophy, and opening angle of zero-stress state of arteries following aortic constriction. J Biomech Eng, 1989, 111(4): 325-335.
10.	Zhao J, Day J, Yuan ZF, et al. Regional arterial stress-strain distributions referenced to the zero-stress state in the rat. Am J Physiol Heart Circ Physiol, 2002, 282(2): H622-629.
11.	盧曉, 王公瑞, 葉志義, 等. 主動脈血管壁三種主要組份對其殘余應力貢獻的分析. 中國生物醫學工程學報, 2001, 20(5): 445-450, 472.
12.	Chiou AC, Chiu B, Pearce WH. Murine aortic aneurysm produced by periarterial application of calcium chloride. J Surg Res, 2001, 99(2): 371-376.
13.	Cardinal JS, Zhan J, Wang Y, et al. Oral hydrogen water prevents chronic allograft nephropathy in rats. Kidney Int, 2010, 77(2): 101-109.
14.	Buchholz BM, Kaczorowski DJ, Sugimoto R, et al. Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant, 2008, 8(10): 2015-2024.
15.	Ohta S. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des, 2011, 17(22): 2241-2252.

1. White JJ, Dalman RL. Gaining new insights into early abdominal aortic aneurysm disease. Perm J, 2008, 12(2): 10-14.
2. Fung YC. What are the residual stresses doing in our blood vessels? Ann Biomed Eng, 1991, 19(3): 237-249.
3. Ohsawa I, Nishimaki K, Yamagata K, et al. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochem Biophys Res Commun, 2008, 377(4): 1195-1198.
4. Shen L, Wang J, Liu K, et al. Hydrogen-rich saline is cerebroprotective in a rat model of deep hypothermic circulatory arrest. Neurochem Res, 2011, 36(8): 1501-1511.
5. Kajiya M, Silva MJ, Sato K, et al. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun, 2009, 386(1): 11-15.
6. Isenburg JC, Simionescu DT, Starcher BC, et al. Elastin stabilization for treatment of abdominal aortic aneurysms. Circulation, 2007, 115(13): 1729-1737.
7. 吳曉飛, 張健, 辛世杰, 等. 雌激素在大鼠腹主動脈瘤形成中的作用. 中華醫學雜志, 2007, 87(7): 471-474.
8. Fung YC, Liu SQ. Changes of zero-stress state of rat pulmonary arteries in hypoxic hypertension. J Appl Physiol, 1991, 70(6): 2455-2470.
9. Liu SQ, Fung YC. Relationship between hypertension, hypertrophy, and opening angle of zero-stress state of arteries following aortic constriction. J Biomech Eng, 1989, 111(4): 325-335.
10. Zhao J, Day J, Yuan ZF, et al. Regional arterial stress-strain distributions referenced to the zero-stress state in the rat. Am J Physiol Heart Circ Physiol, 2002, 282(2): H622-629.
11. 盧曉, 王公瑞, 葉志義, 等. 主動脈血管壁三種主要組份對其殘余應力貢獻的分析. 中國生物醫學工程學報, 2001, 20(5): 445-450, 472.
12. Chiou AC, Chiu B, Pearce WH. Murine aortic aneurysm produced by periarterial application of calcium chloride. J Surg Res, 2001, 99(2): 371-376.
13. Cardinal JS, Zhan J, Wang Y, et al. Oral hydrogen water prevents chronic allograft nephropathy in rats. Kidney Int, 2010, 77(2): 101-109.
14. Buchholz BM, Kaczorowski DJ, Sugimoto R, et al. Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant, 2008, 8(10): 2015-2024.
15. Ohta S. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des, 2011, 17(22): 2241-2252.

《中國修復重建外科雜志》

氫氣飽和生理鹽水對腹主動脈瘤大鼠主動脈殘余應力的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

《中國修復重建外科雜志》

氫氣飽和生理鹽水對腹主動脈瘤大鼠主動脈殘余應力的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料