Study on biocompatibility of carbon-based composites_Journal of Biomedical Engineering

Authors：

YIN Yanxiong ¹ ,  YU Shu ¹ , LI Yunping ¹ , WU Qiang ¹ , LI Xiao ¹ , ZHONG Hui ² , DENG Youwen ³ , XIAO Tao ³ , LIU Lihong ³ , GUO Xiaoning ³

1. Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, P.R.China;
2. School of Life Sciences, Central South University, Changsha 410013, P.R.China;
3. Second Xiangya Hospital, Central South University, Changsha 410011, P.R.China;

Corresponding?author：

YU Shu, Email: yushu@csu.edu.cn

Keywords：

carbon-based composites; biocompatibility; particles shedding

DOI：

10.7507/1001-5515.201708014

Video：

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Silicon carbide (SiC) film and silicon dioxide (SiO₂) film were deposited on the surface of carbon/carbon composite (C/C) by low pressure chemical vapor deposition (LPCVD). The biocompatibility of the three carbon-based composites, e. g. C/C, C/C-SiC, C/C-SiO₂ were investigated by cytotoxicity test, cell direct contact and cell adhesion experiments. Cytotoxicity, cell direct contact and cell adhesion showed that the three materials had no toxic effect on mouse fibroblasts (L929 cells). However, the particles dropped off from the three materials had a great impact on evaluation accuracy of the thiazolyl blue (MTT) test. More the particles were lost, more growth inhibition to L929 cells. The evaluation accuracy of MTT method can be kept with the filtered extract of materials. Furthermore, the results of surface particles shedding experiment showed that the amount of surface particles shed from C/C-SiO₂ was the most, followed by C/C and C/C-SiC in 72 hours. Particles shedding curves showed there was a peak reached at eighth hour and then declined to the thirty-sixth hour. The filtrate analysis showed that there was no ion exchange between the three materials and simulated body fluid (SBF) solution. The results of this study on biocompatibility of carbon-based composites have certain guiding significance for their future application in clinical filed.

Citation： YIN Yanxiong, YU Shu, LI Yunping, WU Qiang, LI Xiao, ZHONG Hui, DENG Youwen, XIAO Tao, LIU Lihong, GUO Xiaoning. Study on biocompatibility of carbon-based composites. Journal of Biomedical Engineering, 2018, 35(5): 740-748. doi: 10.7507/1001-5515.201708014 Copy

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8.	方鐵鈞, 周群, 狄麗莎, 等. 碳纖維增強碳與碳化硅雙基體陶瓷基復合材料作為口腔種植體材料的細胞毒性. 中國醫科大學學報, 2011, 40(5): 417-421.
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11.	倪昕曄, 湯曉斌, 林濤, 等. 炭/炭復合材料的生物學安全性評價研究. 國際生物醫學工程雜志, 2011, 34(6): 340-343.
12.	袁小兵, 陳嵐, 孫宏偉, 等. 炭質股骨頭生物相容性的動物實驗. 中國組織工程研究, 2009, 13(38): 7503-7506.
13.	van Zlverden M, Granum B. Adjuvant activity of particulate pollutants in different mouse models. Toxicology, 2000, 152(1/3): 69-77.
14.	鄧紅平, 宋偉民. 炭黑顆粒毒性作用的研究進展. 中華勞動衛生職業病雜志, 2009, 27(6): 372-374.
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16.	徐鶯鶯, 林曉影, 陳春英. 影響納米材料毒性的關鍵因素. 科學通報, 2013, 58(24): 2466-2478.
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19.	Lao Fang, Li Wei, Han Dong, et al. Fullerene derivatives protect endothelial cells against NO-induced damage. Nanotechnology, 2009, 20(22): 225103.
20.	Zhou Hejiang, Zhao Kai, Li Wei, et al. The interactions between pristine graphene and macrophages and the production of cytokines/chemokines via TLR- and NF-kappa B-related signaling pathways. Biomaterials, 2012, 33(29): 6933-6942.
21.	于成, 趙衛生, 賈偉. 生物醫用復合材料的研究進展. 玻璃鋼/復合材料, 2012, (2): 78-81.
22.	中國國家標準委員會. GB/T16886.5-2003 醫療器械生物學評價第12部分:樣品制備與參照樣品. 北京: 中國標準出版社, 2005.
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24.	中國國家標準委員會. GB/T16886.17-2005醫療器械生物學評價第17部分:可瀝濾物允許限量的建立. 北京: 中國標準出版社, 2005.
25.	中國國家標準委員會. GB/T16886.11-1997醫療器械生物學評價第11部分:全身毒性實驗. 北京: 中國標準出版社, 1997.
26.	中國國家標準委員會. GB/T16886.5-2003醫療器械生物學評價第5部分:體外細胞毒性實驗. 北京: 中國標準出版社, 2003.
27.	鄭旭, 王莉芳, 陳芳, 等. MTT法評價4種醫用材料的細胞毒性. 西北藥學雜志, 2011, 26(5): 363-364.
28.	Wang B L, Li L, Zheng Y F. In vitro cytotoxicity and hemocompatibility studies of Ti-Nb, Ti-Nb-Zr and Ti-Nb-Hf biomedical shape memory alloys. Biomedical Materials, 2010, 5(4): 044102.
29.	International Organization for Standard. Implants for surgery-in vitro evaluation for apatite-forming ability of implant materials. ISO, 2007: 23317.
30.	毛菲菲, 陳書慧, 聶飛龍, 等. 成骨細胞和成纖維細胞黏附及增殖:不同寬度表面均一平行溝槽的影響. 中國組織工程研究, 2014, 18(51): 8243-8247.
31.	吳強, 于澍, 李云平, 等. C/C復合材料的非晶SiO₂涂層制備及其生物活性. 復合材料學報, 2017, 34(7): 1582-1588.
32.	Li Hejun, Zhang Leilei, Lu Jinhua, et al. Wear mechanism of biomedical carbon/carbon composites for artificial hip joints. Journal of Inorganic Materials, 2010, 25(9): 999-1002.
33.	Howling G I, Ingham E, Sakoda H, et al. Carbon-carbon composite bearing materials in hip arthroplasty: analysis of wear and biological response to wear debris. J Mater Sci Mater Med, 2004, 15(1): 91-98.

1. Nine M J, Choudhury D, Hee A C, et al. Wear debris characterization and corresponding biological response: artificial hip and knee joints. Materials (Basel), 2014, 7(2): 980-1016.
2. Steinberg E L, Rath E, Shlaifer A, et al. Carbon fiber reinforced PEEK optima-a composite material biomechanical properties and wear/debris characteristics of CF-PEEK composites for orthopedic trauma implants. J Mech Behav Biomed Mater, 2013, 17(1): 221-228.
3. Baker D, Kadambande S S, Alderman P M. Carbon fibre plates in the treatment of femoral periprosthetic fractures. Injury- International Journal of the Care of the Injured, 2004, 35(6): 596-598.
4. Coelho P G, Granjeiro J M, Romanos G E, et al. Basic research methods and current trends of dental implant surfaces. J Biomed Mater Res B Appl Biomater, 2009, 88B(2): 579-596.
5. 張磊磊, 李賀軍, 李克智, 等. 炭/炭復合材料表面粗糙度對成骨細胞生長行為的影響. 無機材料學報, 2008, 23(2): 341-345.
6. Wan H, Williams R L, Doherty P J, et al. The cytotoxicity evaluation of Kevlar and silicon carbide by MTT assay. J Mater Sci Mater Med, 1994, 5(6/7): 441-445.
7. Lapp C A, Schuster G S. Effects of DMAEMA and 4-methoxyphenol on gingival fibroblast growth, metabolism, and response to interleukin-1. J Biomed Mater Res, 2002, 60(1): 30-35.
8. 方鐵鈞, 周群, 狄麗莎, 等. 碳纖維增強碳與碳化硅雙基體陶瓷基復合材料作為口腔種植體材料的細胞毒性. 中國醫科大學學報, 2011, 40(5): 417-421.
9. 熊信柏, 李賀軍, 黃劍鋒, 等. 生物醫用碳/碳復合材料碳化硅涂層的研究. 西北工業大學學報, 2003, 21(3): 356-359.
10. 段海英, 高少懷. 3種材料纖維樁細胞毒性評價. 口腔醫學研究, 2012, 28(6): 516-518.
11. 倪昕曄, 湯曉斌, 林濤, 等. 炭/炭復合材料的生物學安全性評價研究. 國際生物醫學工程雜志, 2011, 34(6): 340-343.
12. 袁小兵, 陳嵐, 孫宏偉, 等. 炭質股骨頭生物相容性的動物實驗. 中國組織工程研究, 2009, 13(38): 7503-7506.
13. van Zlverden M, Granum B. Adjuvant activity of particulate pollutants in different mouse models. Toxicology, 2000, 152(1/3): 69-77.
14. 鄧紅平, 宋偉民. 炭黑顆粒毒性作用的研究進展. 中華勞動衛生職業病雜志, 2009, 27(6): 372-374.
15. Shvedova A A, Kagan V E, Fadeel B. Close encounters of the small kind: adverse effects of man-made materials interfacing with the nano-cosmos of biological systems. Annu Rev Pharmacol Toxicol, 2010, 50(1): 63-88.
16. 徐鶯鶯, 林曉影, 陳春英. 影響納米材料毒性的關鍵因素. 科學通報, 2013, 58(24): 2466-2478.
17. Sharifi S, Behzadi S, Laurent S, et al. Toxicity of nanomaterials. Chem Soc Rev, 2012, 41(41): 2323-2343.
18. Xia Tian, Kovochich M, Brant J, et al. Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett, 2006, 6(8): 1794-1807.
19. Lao Fang, Li Wei, Han Dong, et al. Fullerene derivatives protect endothelial cells against NO-induced damage. Nanotechnology, 2009, 20(22): 225103.
20. Zhou Hejiang, Zhao Kai, Li Wei, et al. The interactions between pristine graphene and macrophages and the production of cytokines/chemokines via TLR- and NF-kappa B-related signaling pathways. Biomaterials, 2012, 33(29): 6933-6942.
21. 于成, 趙衛生, 賈偉. 生物醫用復合材料的研究進展. 玻璃鋼/復合材料, 2012, (2): 78-81.
22. 中國國家標準委員會. GB/T16886.5-2003 醫療器械生物學評價第12部分:樣品制備與參照樣品. 北京: 中國標準出版社, 2005.
23. 中國國家標準委員會. GB/T16886.14-2003醫療器械生物學評價第14部分:陶瓷降解產物的定性與定量. 北京: 中國標準出版社, 2003.
24. 中國國家標準委員會. GB/T16886.17-2005醫療器械生物學評價第17部分:可瀝濾物允許限量的建立. 北京: 中國標準出版社, 2005.
25. 中國國家標準委員會. GB/T16886.11-1997醫療器械生物學評價第11部分:全身毒性實驗. 北京: 中國標準出版社, 1997.
26. 中國國家標準委員會. GB/T16886.5-2003醫療器械生物學評價第5部分:體外細胞毒性實驗. 北京: 中國標準出版社, 2003.
27. 鄭旭, 王莉芳, 陳芳, 等. MTT法評價4種醫用材料的細胞毒性. 西北藥學雜志, 2011, 26(5): 363-364.
28. Wang B L, Li L, Zheng Y F. In vitro cytotoxicity and hemocompatibility studies of Ti-Nb, Ti-Nb-Zr and Ti-Nb-Hf biomedical shape memory alloys. Biomedical Materials, 2010, 5(4): 044102.
29. International Organization for Standard. Implants for surgery-in vitro evaluation for apatite-forming ability of implant materials. ISO, 2007: 23317.
30. 毛菲菲, 陳書慧, 聶飛龍, 等. 成骨細胞和成纖維細胞黏附及增殖:不同寬度表面均一平行溝槽的影響. 中國組織工程研究, 2014, 18(51): 8243-8247.
31. 吳強, 于澍, 李云平, 等. C/C復合材料的非晶SiO₂涂層制備及其生物活性. 復合材料學報, 2017, 34(7): 1582-1588.
32. Li Hejun, Zhang Leilei, Lu Jinhua, et al. Wear mechanism of biomedical carbon/carbon composites for artificial hip joints. Journal of Inorganic Materials, 2010, 25(9): 999-1002.
33. Howling G I, Ingham E, Sakoda H, et al. Carbon-carbon composite bearing materials in hip arthroplasty: analysis of wear and biological response to wear debris. J Mater Sci Mater Med, 2004, 15(1): 91-98.

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Study on biocompatibility of carbon-based composites

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