摘要:目的:動態觀察大鼠腦出血后血腫周圍組織補體激活與細胞凋亡的規律。方法:用膠原酶注入到大鼠尾狀核的方法制作腦出血模型。將大鼠分為腦出血、假手術組、正常組3組。采用蘇木素伊紅(HE) 染色、免疫組織化學染色及原位末端脫氧核苷酸轉移酶介導的dUTP 缺口末端標記法(TUNEL)分別觀察各組在腦出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d時血腫周圍補體C3、促凋亡基因(Bax)、抑凋亡基因(Bclxl)及TUNEL的表達。結果補體C3的表達峰值在24~48 h;TUNEL、Bax蛋白表達術后12h增加,48~72 h達高峰,而Bclxl蛋白表達高峰在48h。結論:大鼠腦出血后血腫周圍組織補體C3的表達增加與細胞凋亡的演變趨勢一致,C3與凋亡有相關。Abstract: Objective: To study the complement activation and apoptosis regular genes changes in the tissues of the perihematoma of intracerebral hemorrhage (ICH) in rats. Methods: Intracerebral hemorrhage was induced in rats by injection of bacterial collagenase into the caudate nucleus. Histopathological changes were studied in 6 h,12 h, 24 h, 2 d, 3 d, 5 d, 7 d after the injection. The immunohistochemistry and TUNEL analysis were performed. The expression of complement factor C3, the TUNELpositive cells, the proapoptotic gene expression (Bax) and the antiapoptotic gene (Bclxl) were examined. Results: The expression of C3 increased to its maximum between 2448 h. The TUNELpositive cells and Bax protein expression increased gradually and reached the peak level between 4872 h. The Bclxl protein reached the peak level at 48 h. The correlation analysis showed that the quantity of C3 was positively related to that of the TUNELpositive cells, but the bax protein was not related to Bclxl protein. Conclusion: The expression of complement factor C3 may contributes to the nerve injury after cerebral hemorrhage and relate to the apotosis in the tissues surrounding the hametoma in rats.