Thirty seven rabbits were randomly divided into three groups fed with different feeds,the basic feed group as a control,high cholesterol group(feed containing 1.2% cholesterol)and high cholesterol feed added to Dong Li San which is a chinese medicine that can promote the contracition of gallbladder.All animals were fed with the corresponding feeds for four weeks.The results showed that 12 out of 14 rabbits in the high cholesterol group developed gallstone and 2 of 10 rabbits in the high cholesterol and Dong Li San feeding group had gallstone formation.The latter showed much higher emptying rate of gallbladder,lower level of mucin in the bile and much lower resistance to the cystic duct than those of the former.The results suggest that Dong Li San can effectively prevent gallstone formation by promoting gallbladder contraction ,reducing mucin in bile,decreasing cystic duct resistance and promoting the emptying capacity of gallbladder.