Objective To review the regulating effects of estrogen on endothel ial cell functions, the involved endothel ial progenitor cells (EPCs), and the related VEGF, and explore the mechanism of estrogen participating in new vesselformation on wound basement and wound heal ing. Methods Recent l iterature about biological effects of estrogen oncapillary vessel formation was reviewed. Results The formation of new vessel in the wound’s granulation tissue contained vasculogenesis and vascularization, and the new vessels could transport the oxygen and nutrient for the metabol ism of the local heal ing tissue. The estrogen effected vascular endothel ial cells through its receptors. Peripheral vascular EPCs differentiated into endothel ial cells and participated into the formation of new vessels. Estradiol exerted influences on the dynamics of vascular EPCs and the neovascularization. VEGF was a key mediator in the processes of estradiol regulating angiogenesis. Conclusion Understanding the molecular mechanisms that regulate vessel formation in wound heal ing, especially how estrogen modulates its receptor and angiogenic factor, may provide new approaches for managing wound heal ing.