Objective To explore the effect of a teamwork intervention during their rehabilitation process for patients who had undergone an amputatation after an earthquake. Methods A total of 85 patients undergoing amputation were included in the study. A rehabilitation team consisting of orthopaedic doctors, orthopaedic nurses, physical therapists, professional therapists, psychology consultants, volunteers, social workers, patients and their relatives participated in the comprehensive rehabilitation program. The outcome of this teamwork intervention was evaluated. Results Statistically significant improvement was observed in terms of patients’ physiological, psychological and social roles after the teamwork intervention ( Plt;0.05). Conclusion The teamwork intervention can improve the overall outcome of rehabilitation, including quality of life, in amputated patients following an earthquake.