Human fibroblasts and human epidermal keratinocytes were used for culture. Chitosan solution were added in the culture solution(DMEM). After 72 hours, the fibroblasts showed rapid growth in the control culture without Chitosan, But the numbers of human fibroblasts from growth was decreased as the concentration of Chitosan was increasing. On the contrary the human epidermal keratinocytes growed more rapidly in the culture with Chitosan than in the culture without Chitosan. The results showed that Chitosan inhibited the growwth of human fibroblast and stimulated the growth of human epidermal keratinocyte .
AbstractThe Staphyloccus epidermidis,pseudomonas aeruginosa,Staphylococcus aureus,Escherichi時 acoli,and Candida al bicans were selected as tested micrcorganisms. According to doubling dilutionrule,the chitosan solution of different dosage was added in the culture solution and kept at 37℃constant tcmporature for 18 hours. The smallest bacteriortatic concentration of the chitosan solutionwas 0.016%for Saphylococcus aureus,0.008%for Staphylococcus epidermidis, 0.032%forEscherichia coli,0...