ObjectiveTo study the effects of newcastle disease virus (NDV) on tumor infiltrating lymphocyte (TIL). MethodsTILs were dissociated from 6 cases of primary hepatic carcinoma. Each case was divided into 3 portions equally, and was added 20 μl NDV with HA titer of 1∶160 (NDV group), rIL2 with the end concentration of 1 000 u/ml (rIL2 group) and 0.9% sodium chloride (control group) respectively, then cultured in the condition of 37℃，5% CO2. CD3, CD4 and CD8 were assayed by flow cytometry. The contents of IL2, IFNγ and TNFβ in supernate of NDV group and control group were examined by ELISA.ResultsCoupled material revealed that CD3 and CD4 were significantly higher in NDV group than in the other two groups (P＜0.05, P＜0.01). CD8 had no significant changes in three groups. The contents of IL2, IFNγ and TNFβ in NDV group were significantly higher than those in control group (P＜0.01, P＜0.01 and P＜0.05 respectively). ConclusionNDV can activate TIL directly.