Objective To investigate the effect of myeloid derived suppressor cells ( MDSCs) on airway inflammation of asthmatic mice. Methods Five male BALB/ c mice aged 6 weeks were used for preparing 4T1 tumor bearing mice. Thirty female BALB/ c mice aged six weeks were randomly divided into a normal control group, an athmatic model group, and a cell transplantation group. The MDSCs were separated frommyeloid tissue of tumor-bearing mice using amagnetic cell sorting systemand cultured in RPMI medium 1640 containing GM-CSF. The morphologic characteristics of these cells were observed under lightmicroscope and the phenotypic figures were analyzed with flow cytometry. The mice in the model group and the cell transplantation group were sensitized by ovalbumin and then stimulated with nebulized ovalbumin. The mice in the cell transplantation group were intravenously administered MDSCs which purified by magnetic cell sorting system at 10 days after sensitization. The airway inflammation was evaluated by HE staining. The total and differential cell counts in bronchoalveolar lavage fluid ( BALF) were measured.Results The neutrophil and eosinophil infiltration in pulmonary tissue was dramatically increased in the model group, but not observed in the normal control group and was much milder in the cell transplantation group. The total cell count, the eosinophil and lymphocyte counts in BALF of the model group and the cell transplantation group were significantly higher than those of the normal control group( P lt; 0. 05) , and the number of eosinophils in BALF of the cell transplantation group was decreased when compared with that of the model group( P lt;0. 05) . Conclusion MDSCs via intravenous infusion can effectively suppress airway inflammation in a mouse asthma model.
Objective To evaluate the effect of transpancreatic pre-cut sphincterotomy on recurrence after endoscopic retrograde cholangiopancreatography (ERCP) for common bile duct stone. MethodsA total of 102 patients with common bile duct stone who underwent ERCP stone extraction at Beijing Aerospace General Hospital from January 2022 to January 2024 were retrospectively collected. Using propensity score matching to adjust equilibrium, 45 patients were divided into the transpancreatic pre-cut sphincterotomy group and 45 were in the control group (standard cannulation method). Compare stone recurrence rate, surgical parameters, liver function indicators, inflammatory factors, oxidative stress markers and adverse events between the two groups. ResultsThe improvement of postoperative indicators of the transpancreatic pre-cut sphincterotomy group including total bilirubin [(5.18±1.30) μmol/L vs. (1.64±0.88) μmol/L, P<0.001], alkaline phosphatase [(16.45±4.82) U/L vs. (9.12±3.04) U/L, P<0.001], alanine aminotransferase [(6.83±2.21) U/L vs. (3.57±1.92) U/L, P<0.001], C-reactive protein [(3.34±1.15) mg/L vs. (1.53±0.87) mg/L, P<0.001], interleukin-6 [(5.72±1.90) pg/mL vs. (3.02±1.48) pg/mL, P<0.001], tumor necrosis factor-α [(6.84±2.35) pg/mL vs. (3.44±1.98) pg/mL, P<0.001], malondialdehyde [(2.40±0.79) nmol/mL vs. (1.16±0.72) nmol/mL, P<0.001], superoxide dismutase [(–17.36±5.52) U/mL vs. (–7.43±4.46) U/mL, P<0.001] were superior to the control group. The overall stone recurrence rate was lower in the transpancreatic pre-cut sphincterotomy group [11.11%(5/45) vs. 33.33%(15/45), P=0.011]. The adverse events rate was decreased in the transpancreatic pre-cut sphincterotomy group [22.22% (10/45) vs. 8.89% (4/45), P=0.037], accompanied by a reduced risk of adverse events [RR=0.523, 95%CI (0.280, 0.964)]. ConclusionTranspancreatic pre-cut sphincterotomy effectively reduces the risk of stone recurrence and adverse events following ERCP for common bile duct stone, while promoting postoperative recovery of liver function.