Objective To improve the method of hard tissue slicing for research of bone tissue engineering.Methods The method of hard tissue slicing especially the key techniqueslice mounting had been studied and improved. Results The experiments showed that our research improved techniques in hard tissue slicing not only in keeping the characteristics of hard tissue slicing, easily stain with HE, Masson, immunohistochemistry, and distinguishing the differences among tissues easily, but also in overcoming the shortcoming of issue shedding easy to happen in the general hard tissue slicing. Conclusion The improved techniquesin hard tissue slicing is a more useful hard tissue slicing techniques.