The number of new cancer cases in China has been increasing year by year, but with the continuous innovation of medical technology, cancer is gradually becoming a chronic disease. The contradiction between the increasingly large tumor patient population and limited medical resources is becoming more prominent, so the mode of daytime chemotherapy has been widely promoted. This article is based on the operational practice of the “one-stop” daytime chemotherapy center at the Second Affiliated Hospital of Zhejiang University School of Medicine. It shares experiences in management mode and system construction (such as spatial layout, personnel structure, operation process, emergency process, job responsibilities, quantitative indicators), showcases the construction achievements of the “one-stop” daytime chemotherapy center, and proposes suggestions for improving the centralized daytime chemotherapy mode.