【Abstract】ObjectiveTo inquire the therapeutic effect of retroperitoneal splenic autotransplantation combined with lower esophageal transection in the treatment of hepatic cirrhosis induced portal hypertension with randomized comparasion.MethodsThe hepatic cirrhosis induced portal hypertension patients with Child A or B grade of liver function were randomly divided into splenic autotransplantation group and splenectomy group.In the splenic autotransplantation group, retroperitoneal transplantation of pedicled autosplenic tissue combined with modified lower esophageal transection was performed,while in the splenectomy group, splenectomy combined with modified lower esophageal transection was conducted.The general conduction, splenic scanning, liver function, serum tuftsin and IgM levels in patients were observed 2 to 6 months after operation, and compared with those before operation. ResultsOne patient died in the splenectomy group on the 6th postoperative day, rebleeding occurred in one case of the splenic autotransplantation group. The levels of tuftsin and IgM in splenic autotransplantation group were higher than those of splenectomy group after operation, with significant difference (P＜0.01). The liver function between two groups showed no difference (Pgt;0.05).ConclusionSpleen autografts could maintain the basic immune function of spleen and survive for a long time.
目的:探討Livin基因在人膽管癌組織及膽管癌細胞系中的表達情況及其與膽管癌發 生發展之間的關系。方法:采用免疫組織化學技術（SP法）檢測Livin基因蛋白在45例人膽管 癌標本及及40例癌旁膽管組織、20例正常膽管組織標本中的表達；同時采用RTPCR法及SP 法檢測了Livin基因mRNA和蛋白在人膽管癌細胞系QBC939及非腫瘤細胞系HT1080中表達。 結果:Livin在膽管癌組織中表達陽性率為57.8%，而癌旁膽管組織、非癌膽管組織中未能檢 測到Livin表達。Livin表達與性別、年齡、腫瘤大小及腫瘤分化程度無關。在有淋巴結轉組 中，Livin陽性表達率（70.4%）明顯高于無淋巴結轉移組（38.9%）。在人膽管癌細胞QBC939 中，Livin mRNA及蛋白均特異性表達，而非腫瘤細胞系HT1080未見Livin表達。結論:Livin 基因在人類膽管癌組織和細胞系中選擇性高表達，其可能與膽管癌發生、發展及預后密切相 關。