目的:探討甲狀腺功能低下的早產兒血清游離三碘甲狀腺原氨酸(Free trilute,FT3)、游離甲狀腺激素(Free thyroxin,FT4)、促甲狀腺激素(Thyroid stimulation hormone,TSH)水平進行動態態變化及臨床意義。方法:我院在2007年11月至2008年4月住院的32例診斷為甲狀腺功能低下的早產兒為研究對象,應用放射免疫法檢測生后第1天,第7天,第15天,1月和2月血清FT3、FT4、TSH水平。結果:32例診斷為甲狀腺功能低下的早產兒中,有窒息史的22例,呼吸窘迫綜合癥(Respiratory distress syndrome,RDS)10例,單純性早產5例;出現少吃少動臨床癥狀6例;生后各時期的TSH變化沒有統計學差異,生后1、5天與生后1、2月FT3、FT4則可見Plt;0.05具有統計學差異。結論:有窒息和RDS的早產兒中,容易發生甲狀腺功能降低,以低甲狀腺素血癥最為常見,使用左旋甲狀腺素后,FT-3、FT-4水平可迅速恢復正常。如果同時存在TSH增高,則TSH水平恢復較慢。
摘要:目的:探討卡配因抑制劑3（MDL28170）對新生大鼠缺氧缺血性腦損傷(HIBD)神經細胞凋亡的影響。方法:建立新生SD大鼠HIBD模型,治療組于缺養缺血后即刻、2 h、4 h腹腔內注射MDL28170,對照組及手術組同時予生理鹽水。缺氧缺血后24 h用免疫組化方法觀察大腦皮質及海馬CA1區Caspase3 蛋白表達、TUNEL法檢測細胞凋亡,觀察組織病理改變并計算海馬神經元死亡數,透射電鏡觀察細胞超微結構。結果:缺氧缺血后24 h缺血側大腦皮質及海馬CA1區Caspase3和TUNEL陽性細胞數較對照組明顯增加,透射電鏡證實有凋亡細胞;MDL28170可減少陽性細胞數量,抑制神經元死亡,差異有顯著性(Plt;0.05)。結論:MDL28170可通過抑制神經凋亡而對新生大鼠HIBD具有一定保護作用。Abstract: Objective: To investigate the effect of (Calpain inhibitor3) MDL28170 on neural apoptosis in a neonatal model of hypoxicischemic brain damage (HIBD). Methods: A neonatal model of HIBD was established, 7dayold SD rats were divided into three groups. The treatment group received MDL28170(ip) at 0 h,2 h,4 h after HI, whereas the other two groups were administered normal saline simultaneously. The expression of caspase3 (by immunohistochemistry), neural apoptosis (by TUNEL) in cortex and hippocampus ipsilateral to the insult were observed 24 h after HI; hippocampal CA1 neural loss and electromicroscopic changes were assessed at the same time. Results: Apoptotic body was observed by electromicroscopy. Caspase3 positive cells and apoptotic cells increased significantly in the ipsilateral cortex and hippocampal CA1 region compared to the control, and MDL28170 reduced the number of positive cells, attenuated CA1 neural loss with significance (Plt;0.05). Conclusion: It is suggested that MDL28170 may protect the brain of neonatal rats after HIBD by suppressing neural apoptosis.