Objective To discuss the method of constructingbiomechanical model of rabbit femur.Methods The sample of rabbit femur was prepared as follows:firstly，femur section images were obtained，then the image wasput into the computer and processed to get the boundary contour line; secondly, through programming the contour line coordinate for modeling was obtained, then the data were put into the model software to find the threedimensional entity model. Results Whole three-dimensional model of rabbit femur was constructed. It simulated actually dissection form of femur. Conclusion The establishment of the model lays a foundation for ascertaining optimal parameter of vibration improving bone minerydensity by finite element analysis.