Objective To evaluate the biocompatibility of a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering. Methods Osteoblasts combined with NBM in vitro were cultured. The morphological characteristics was observed; cell proliferation, protein content and basic alkaline phosphatase(ALP) activity were measured. NBM combined with osteoblasts were implanted into the skeletal muscles of rabbits and the osteogenic potential of NBM was evaluated through contraat microscope, scanning electromicroscope and histological examination. In vitro osteoblasts could attach and proliferate well in the NBM, secreting lots of extracellular matrix; NBM did not cause the inhibition of proliferation and ALP activity of osteoblasts. While in vivo experiment of the NBM with osteoblasts showed that a large number of lymphacytes and phagocytes invading into the inner of the material in the rabbit skeletalmuscle were seen after 4 weeks of implantation and that no new bone formation was observed after 8 weeks. Conclusion This biocompat ibility difference between in vitro and in vivo may be due to the immunogenity of NBM which causes cellular immuno reaction so as to destroy the osteogenic environment. The immunoreaction between the host and the organic-inorganic composite materials in tissue engineering should be paid more attention to.
ObjectiveTo review the research progress of medicine biomaterials in prevention and treatment of adhesion after tendon injury, and to provide reference for clinical treatment.MethodsThe literature on the application of medical biomaterials in the prevention and treatment of tendon adhesions in recent years was reviewed, and the biological process, treatment methods, and current status of tendon adhesions were summarized.ResultsTendon adhesion as part of the healing process of the tendon is the biological response of the tendon to the injury and is also a common complication of joint dysfunction. Application of medical biomaterials can achieve better biological function of postoperative tendon by reducing the adhesion of peritendon tissues as far as possible without adversely affecting the tendon healing process.ConclusionThe use of medical biomaterials is conducive to reduce the adhesion of tendon after operation, and the appropriate anti-adhesion material should be selected according to the patients’ condition and surgical needs.
Objective To summarize the latest developments in silk protein fiber as biomaterials and their applications in tissue engineering. Methods Recent original literature on silk protein fiber as biomaterials were reviewed, illustrating the properties of silk protein fiber biomaterials. Results The silk protein fiber has the same functions of supporting the cell adhesion, differentiation and growth as native collagen, and is renewed as novel biomaterials with good biocompatibility, unique mechanical properties and is degradable over a longer time. Conclusion Silk protein-fiber can be used as asuitable matrix for three dimensional cell culture in tissue engineering. It has a great potential applications in other fields.
Objective To investigate the currently-used biomaterials in reparative and reconstructive surgery and to clarify the relationship between the development of biomaterials and the progress of reparative and reconstructive surgery. Methods Based on the author’s many years’ scientific researches and combined with the literature available at home and abroad, the biomaterials used in the clinical practice, and their kinds and application fields were summarized. Results Based on the sufficient knowledge of the component structure of biomaterials and the patient’s pathological status, the matching biomaterials could be designed and developed. According to the analysis on some common defects occurring in the skin, bone, cartilage, vocalcord, nerve, and drum membrane, the methods of repairing the defects with biomaterials that we had developed, such as collagen, chitosan, and hyaluronate, achieved good results. Conclusion The rapid development of biomaterials can greatly promote progress of reparative and reconstructive surgery andthere exists a dependence relationship between the two. The related histological responses and the importance of biological estimation after implantation of biomaterials should be emphasized.
In search of a rapid method for vascular anastomosis with high quality,a compatative study was carried out to observe the results of laser welding of the saphenous artery of rabbit(0.45-0.85 indiameter)with the use of a degradable intraluninal bionterial support and the traditional method of suture anastomosis. The results showed that there was no significant difference observed between the two groups in the immediate and long patency rated and the occurrence of stenosis,However,the time ...
Objective To investigate the latest development of tissue engineeredregenerative medicine in industrialization, with the intention to direct work in practical area. Methods A complete insight of regenerative medicine in industrialization was obtained through referring to update publications, visiting related websites, as well as learning from practical experience. Results The aerial view of the future of regenerative medicine was got based on knowledge of four different tissue engineering projects. Conclusion All present efforts should be devoted to regenerative medicine area meeting the industrialized trends.
ObjectiveTo summarize the developments of oxygen-generating materials as biomaterials and its applications in tissue engineering. MethodsThe recent literature on oxygen-generating materials as biomaterials was extensively reviewed, illustrating the properties and applications of oxygen-generating materials in tissue engineering. ResultsOxygen-generating materials as biomaterials have good biocompatibility and degradability. It supports the cell adhesion differentiation and growth. It is used for repairing liver, pancreas, myocardium, and so on. After modification, oxygen-generating materials can be extensively used in tissue engineering. ConclusionOxygen-generating materials is a good biomaterial, which has a great potential applications in tissue engineering.
OBJECTIVE: To discuss the approaches of tissue engineered blood vessels (TEBV) reconstruction. METHODS: The recent literatures about TEBV were widely reviewed. We summarized various types of biomaterials served as scaffold for TEBV and evaluated the construction model of TEBV. And the biological properties of some TEBV were compared. RESULTS: Although the final model of construction of TEBV was not clear, reports in the last two years had shown several important advances in this exciting field. CONCLUSION: Mimicry of some or all of the properties of three layers of natural healthy blood vessels has been the strategy of all TEBV approaches.
ObjectiveTo summarize the application status and progress of the strategies to augment tendon-to-bone healing. MethodsThe present researches focused on augmentation of tendon-to-bone healing were extensively reviewed. ResultsThe present strategies to augment healing of tendon-to-bone by enhancing the location environment, and increasing the cell numbers and relative growth factor. The mainly strategies include using calcium phosphate materials, biocompatible scaffolds and glue, growth factors, cell matrix, platelet-rich plasma, and periosteum. Although periosteum have been used in clinical and got some possitive effects, the others still not be used in clinical and needs further studies. ConclusionThere are many strategies to enhance the ability of tendon-to-bone healing, which got some positive results, but results of studies were varied. Thus, further fundamental research and clinical studies are required to achieve the best effects.
Objective To review the research advances of the tracheal prosthesis. Methods The articles concerned in recent years were extensively reviewed. Results There were still many arguments about the use of tracheal substitutes. Avariety of artificial trachea had been designed and assessed, but so far none of them had been satisfactory for clinical use. The failures were mainly due to their high mortality and incidence of complication such as prosthetic defluvium, granuloma formation, local infection, air leakage, anastomotic stenosis or obstruction. Conclusion The major causes of the poor effectiveness by the use of tracheal prosthesis are closely related to its biological compatibilities. The selected biomaterials and the design of prosthesis hold the key to a breakthrough in research and clinical use of tracheal prosthesis.