Drug-coated devices, including drug-coated balloons and drug-eluting stents, are effective tools to overcome restenosis after endovascular treatment of lower extremity peripheral arterial diseases. To use drug-coated devices properly, it is essential to assess the nature of the lower limb arterial lesions, which is a key factor affecting surgical success and postoperative efficacy. Based on a thorough assessment of the lesion characteristics, the most appropriate vessel preparation method can be selected, including the two main methods of balloon dilation and debulking. The purpose of vessel preparation is to fully obtain the lumen of the vessel, improve the compliance of the vascular wall and create conditions for the application of drug-coated devices. Standardized application of drug-coated devices requires an understanding of the performance characteristics of different devices. Drug-coated balloons and drug-eluting stents have different clinical applications, so it is essential to strictly grasp the indications for use, reasonably combine them, and complement their respective advantages. Attention must be paid to controlling technical details during use and managing perioperative medication properly.
Objective To study the inhibitory effect of RNA interference (RNAi) on bcl-2 expression of vascular smooth muscle cells （VSMCs) in rabbit. Methods The expression vector of bcl-2 gene-targeting small interference RNA (pshRNA-bcl-2) was constructed and was transfected into VSMCs by lipofectamine, and the unloaded vector was used as control. The expression of bcl-2 mRNA was identified by RT-PCR and Western blot, respectively. The growth of the transfected VSMCs was examined by MTT. Results The pshRNA-bcl-2 may inhibit the expression of bcl-2 gene at the levels of transcription and translation. There were significant differences (P＜0.01) of the expressions of bcl-2 mRNA between the VSMCs that were transfected with pshRNA-bcl-2 and the ones in plasmid transfected group and control group, respectively. There was a significant difference (P＜0.01) in the growth of VSMCs between the plasmid transfected and the control groups. Conclusion The plasmid containing the small interference RNA of bcl-2 may have an inhibitory effect on the cell growth and endogenous expression of bcl-2 gene at the levels of transcription and translation in VSMCs.