目的 研究人喉表皮癌細胞系Hep-2中乳腺癌易感基因1（BRCAl）、P53結合蛋白1（53BP1）和DNA損傷檢測點介質1（MDC1）的表達及臨床意義。 方法 采用逆轉錄聚合酶鏈式反應檢測BRCA1、53BP1、MDC1在喉癌細胞系Hep-2中mRNA的表達，同時用免疫印跡法檢測蛋白的表達。 結果 在所檢測的人喉癌細胞系Hep-2中BACR1、53BP1、MDC1在基因與蛋白兩個水平均有表達。 結論 BRCA1、53BP1、MDC1可能在喉癌的發生發展中有一定作用。
【Abstract】ObjectiveTo investigate the expressions of hTERT mRNA and BRCA1 protein and to analyze the correlation between these two factors in breast cancer. MethodsThe expression of hTERT mRNA was examined by reverse transcription polymerase chain reaction (RT-PCR). The expression of BRCA1 protein was examined by immunohistochemistry. ResultsThe positive rates of hTERT mRNA and BRCA1 protein were 72.1%（31/43） and 34.9%（15/43） in breast cancer tissue, were 5.0%(2/40) and 77.5%(31/40) in paracancerous breast tissue respectively. Significant difference existed between breast cancer tissue and paracancerous breast tissue (P＜0.05). Significant negative correlation existed between the expression of BRCA1 protein and expression of hTERT mRNA （r=-0.995, P＜0.01）. ConclusionThe expression of hTERT mRNA is upregulated in breast cancer, and expression of BRCA1 protein is downregulated in breast cancer. BRCA1 protein expression may be associated with expression of hTERT mRNA in breast cancer, which may be involved in the carcinogenesis of breast cancer.