【摘要】目的探討乳腺癌根治術式改良的方法及其在減少術后早期并發癥中的意義。方法398例乳腺癌患者，行乳腺癌根治術22例，Patey氏手術83例，Anchincloss手術247例，改良Anchincloss手術46例。比較不同術式術后早期并發癥發生情況。結果乳腺癌根治術術后的早期并發癥發生率明顯高于其它術式（Plt;0.01）； Patey氏手術與Anchincloss手術相比，皮下積液、皮瓣壞死的發生率差異無顯著性意義（Pgt;0.05），但患側上肢水腫的發生率前者高于后者（Plt;0.01）； 改良Anchincloss手術的并發癥發生率明顯低于前3種術式（Plt;0.01），且其手術時間比其它術式平均縮短20 min。結論改良Anchincloss手術能明顯減少術后早期并發癥發生率并縮短手術時間。
Objective To observe the effects of hydrogen peroxide on the expression of transforming growth factorβ1(TGF-β1) and Smad3 protein in A549 cells. Methods A549 cells were cultured with different concentrations of hydrogen peroxide. MTT assay was used to determine the cell growth and survival rates. The level of TGF-β1 and p-Smad3 protein were detected by western blotting. Results It was observed that hydrogen peroxide significantly inhibit proliferation of A549 cells. When the concentration of hydrogen peroxide was 1.0 mmol/L, the inhibition ratio reaches 46.34%, and the level of TGF-β1 and p-Smad3 protein were increased in a time-dependence manner and reached a peak after 24 h, then decreased a little but also remained at high level. Conclusions In the early oxidative damage, A549 cells express high level of TGF-β1 and p-Smad3 protein. It may be relevant to tissue repair and remodeling after lung injury.