Krüppel 樣因子4 在實驗性肺纖維化小鼠肺組織中的表達及意義_《中國呼吸與危重監護雜志》

作者：

陳雪芬 ^1# , 姬文婕 ^1# , 胡道川 ¹ , 馬永強 ¹ , 周欣 ² , 魏路清 ¹

1 中國人民武裝警察部/ 隊后勤學院附屬醫院呼吸與重癥醫學科( 天津300162);;
2 中國人民武裝警察部隊后勤學院附屬醫院心臟中心武警部隊心血管病研究所( 天津300162)通信作者: 魏路清, E-mail: luqing-wei@ 163. com#陳雪芬，姬文婕為共同第一作者;

關鍵詞：

Krüppel 樣因子4 肺纖維化博萊霉素小鼠

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討博萊霉素誘導小鼠肺纖維化模型中, Krüppel 樣因子4( KLF4) 的表達及其意義。
方法 C57BL/6 小鼠隨機分為生理鹽水對照組( Control) 和博萊霉素組( BLM) , 經氣管內注入博萊霉素( 2. 5 mg/kg) 建立實驗性小鼠肺纖維化模型, 注入等量生理鹽水作為對照組, 分別于術后第12 h、1 d、2 d、3 d、7 d、14 d 及28 d 取材, 采用HE 染色和Masson 染色觀察肺組織病理變化及膠原的沉積部位和數量, 實時定量PCR 法和免疫組織化學法分別檢測各組肺組織中KLF4 mRNA 和蛋白表達水平。
結果博萊霉素誘導的小鼠肺纖維化過程中, 第1、2 及3 d 表現為急性炎癥; 第7 d 炎癥加重, 并出現膠原沉積; 第14 d 肺泡結構塌陷, 膠原沉積明顯; 第28 d 肺泡結構基本正常, 炎癥程度和膠原沉積較輕。與對照組相比, 博萊霉素組小鼠肺組織中KLF4 mRNA 的表達水平第1 d 開始升高, 而后降低, 第3 d 達最低后逐漸升高直至第28 d。博萊霉素組小鼠肺組織中KLF4 蛋白的表達趨勢與mRNA 水平基本一致, 第1 d 開始升高, 而后降低, 第3 d 達最低后逐漸增加至第14 d 后再次降低。
結論 KLF4 在博萊霉素致肺纖維化過程中呈現明顯的動態變化, 并可能參與了肺纖維化的發生與發展。

引用本文： 陳雪芬 ,姬文婕,胡道川,馬永強,周欣,魏路清. Krüppel 樣因子4 在實驗性肺纖維化小鼠肺組織中的表達及意義. 中國呼吸與危重監護雜志, 2013, 12(5): 509-512. doi: 復制

1.	McConnell BB, Yang VW. Mammalian Kruppel-like factors in health and diseases. Physiol Rev, 2010, 90: 1337-1381.
2.	Zhou Y, Hofstetter WL, He Y, et al. KLF4 inhibition of lung cancer cell invasion by suppression of SPARC expression. Cancer Biol Ther, 2010, 9: 507-513.
3.	Liao X, Haldar SM, Lu Y, et al. Kruppel-like factor 4 regulates pressure-induced cardiac hypertrophy. J Mol Cell Cardiol, 2010 ,49: 334-338.
4.	Rolipram attenuates bleomycin A5 -induced pulmonary fibrosis in rats. Respirology, 2009, 14: 975 -982.
5.	Todd NW, Luzina IG, Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012, 5: 11.
6.	Artlett CM. Inflammasomes in wound healing and fibrosis. J Pathol, 2013, 229: 157-167 .
7.	Hu B, Wu Z, Liu T, et al. Gut-enriched Kruppel-like factor interaction with Smad3 inhibits myofibroblast differentiation. Am J Respir Cell Mol Biol, 2007 , 36: 78-84.
8.	Liu YN, Abou-Kheir W, Yin JJ, et al. Critical and reciprocal regulation of KLF4 and SLUG in transforming growth factor betainitiated prostate cancer epithelial-mesenchymal transition. Mol CellBiol, 2012 , 32: 941-953.
9.	Feinberg MW, Cao Z, Wara AK, et al. Kruppel-like factor 4 is a mediator of proinflammatory signaling in macrophages. J Biol Chem, 2005, 280: 38247 -38258.
10.	Duffield JS, Forbes SJ, Constandinou CM, et al. Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. J Clin Invest, 2005, 115: 56-65.
11.	Liao X, Sharma N, Kapadia F, et al. Kruppel-like factor 4 regulates macrophage polarization. J Clin Invest, 2011, 121: 2736-2749.
12.	Alder JK, Georgantas RW, 3rd, Hildreth RL, et al. Kruppel-like factor 4 is essential for inflammatory monocyte differentiation in vivo. J Immunol, 2008, 180: 5645-5652.
13.	Degryse AL, Lawson WE. Progress toward improving animal models for idiopathic pulmonary fibrosis. AmJ Med Sci, 2011,341: 444-449.

1. McConnell BB, Yang VW. Mammalian Kruppel-like factors in health and diseases. Physiol Rev, 2010, 90: 1337-1381.
2. Zhou Y, Hofstetter WL, He Y, et al. KLF4 inhibition of lung cancer cell invasion by suppression of SPARC expression. Cancer Biol Ther, 2010, 9: 507-513.
3. Liao X, Haldar SM, Lu Y, et al. Kruppel-like factor 4 regulates pressure-induced cardiac hypertrophy. J Mol Cell Cardiol, 2010 ,49: 334-338.
4. Rolipram attenuates bleomycin A5 -induced pulmonary fibrosis in rats. Respirology, 2009, 14: 975 -982.
5. Todd NW, Luzina IG, Atamas SP. Molecular and cellular mechanisms of pulmonary fibrosis. Fibrogenesis Tissue Repair,2012, 5: 11.
6. Artlett CM. Inflammasomes in wound healing and fibrosis. J Pathol, 2013, 229: 157-167 .
7. Hu B, Wu Z, Liu T, et al. Gut-enriched Kruppel-like factor interaction with Smad3 inhibits myofibroblast differentiation. Am J Respir Cell Mol Biol, 2007 , 36: 78-84.
8. Liu YN, Abou-Kheir W, Yin JJ, et al. Critical and reciprocal regulation of KLF4 and SLUG in transforming growth factor betainitiated prostate cancer epithelial-mesenchymal transition. Mol CellBiol, 2012 , 32: 941-953.
9. Feinberg MW, Cao Z, Wara AK, et al. Kruppel-like factor 4 is a mediator of proinflammatory signaling in macrophages. J Biol Chem, 2005, 280: 38247 -38258.
10. Duffield JS, Forbes SJ, Constandinou CM, et al. Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. J Clin Invest, 2005, 115: 56-65.
11. Liao X, Sharma N, Kapadia F, et al. Kruppel-like factor 4 regulates macrophage polarization. J Clin Invest, 2011, 121: 2736-2749.
12. Alder JK, Georgantas RW, 3rd, Hildreth RL, et al. Kruppel-like factor 4 is essential for inflammatory monocyte differentiation in vivo. J Immunol, 2008, 180: 5645-5652.
13. Degryse AL, Lawson WE. Progress toward improving animal models for idiopathic pulmonary fibrosis. AmJ Med Sci, 2011,341: 444-449.

上一篇
感染后閉塞性細支氣管炎的臨床診治
下一篇
吉非替尼致間質性肺疾病二例并文獻復習

《中國呼吸與危重監護雜志》

Krüppel 樣因子4 在實驗性肺纖維化小鼠肺組織中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

《中國呼吸與危重監護雜志》

Krüppel 樣因子4 在實驗性肺纖維化小鼠肺組織中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料