目的 幽門螺桿菌NCTC11637菌株Hp1501基因進行序列測定，并運用生物信息學軟件對其進行分析。 方法 用聚合酶鏈反應方法從幽門螺桿菌 NCTC11637菌株基因組DNA擴增Hp1501基因，T-A克隆，鑒定后測序，將基因序列向GeneBank提交并申請登錄號，用生物信息學軟件分析其生物學特性。 結果 成功獲取幽門螺桿菌 NCTC11637株Hp1501基因序列，獲得GeneBank登錄號JF820815。軟件分析表明，序列全長為1 164 bp，與幽門螺桿菌國際標準株26695和J99的基因序列一致性為96%～97%，氨基酸序列一致性為97%～98%；軟件預測其編碼的前59個氨基酸為信號肽，其編碼的核心肽為幽門螺桿菌外膜蛋白。 結論 成功測定幽門螺桿菌 NCTC11637菌株Hp1501基因序列并預測出其生物學特性，為進一步研究其功能，闡明其致病機制奠定了基礎。
Objective To determine the sequence of Hp1501 gene from H. pylori NCTC11637 and analyze the sequence with bioinformatics software. Methods Polymerase chain reaction (PCR) was used to amplify the Hp1501 gene from chromosomal DNA of H. pylori NCTC11637. After T-A clone, the amplified DNA sequence was determined and the gene sequence was sent to GeneBank for analysis with bioinformatics software. Results Hp1501 gene from H. pylori NCTC11637 was successfully attained, and the logging number for GeneBank was JF820815. The analysis showed that the gene sequence was 1164 bp in length, 96%-97% identical in DNA sequence and 97%-98% identical in amino acid sequence compared with standard strain 26695 and J99. The forward 59 amino acids were signal peptide and the core peptide was outer membrane protein of H. pylori based on the software prediction. Conclusions The sequence and bionomics of Hp1501 gene from H. pylori NCTC11637 has been determined successfully. It provides a solid base for the further research of the biological function and pathogenicity mechanism of H. pylori.
引用本文： 楊繼文,敬保遷,王朝麗,馮莉. 幽門螺桿菌NCTC11637株Hp1501基因序列的測定與生物信息學分析. 華西醫學, 2012, 27(1): 58-62. doi: CNKI: 51-1356/R.20120115.1546.013 復制