CyclinD1基因擴增及其蛋白表達在乳腺癌中的意義_《中國普外基礎與臨床雜志》

作者：

趙俊玲 , 楊金巧 , 賴毅

華西醫科大學附屬第一醫院普外科(成都610041);

關鍵詞：

乳腺癌 CyclinD1基因 CyclinD1蛋白

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目的探討乳腺癌中CyclinD1基因擴增和其蛋白表達的相關性及其臨床意義。
方法應用半定量PCR和免疫組化技術，檢測和分析了乳腺癌及其癌旁組織、乳腺良性組織及正常乳腺標本中CyclinD1基因的擴增和CyclinD1蛋白的表達情況。
結果 62例乳腺癌中，CyclinD1基因擴增占22.6%(14/62)，蛋白過度表達占48.4%(30/62)，二者有一定相關性，而其它各種乳腺組織的CyclinD1基因擴增及蛋白過度表達與之相比差異有顯著性意義(Ｐ＜0.05)。CyclinD1基因擴增及蛋白過度表達與乳腺癌組織學分級呈明顯正相關，且CyclinD1蛋白過度表達還與雌孕激素受體狀況呈正相關。
結論乳腺癌中CyclinD1基因擴增程度和其蛋白過度表達雖有一定相關性，但并不完全一致，說明還存在著其它導致CyclinD1蛋白過度表達的機理，CyclinD1蛋白的表達可能還受雌激素的調控。對乳腺癌中CyclinD1基因及其蛋白表達的檢測有一定臨床意義。

引用本文： 趙俊玲,楊金巧,賴毅. CyclinD1基因擴增及其蛋白表達在乳腺癌中的意義. 中國普外基礎與臨床雜志, 2000, 7(3): 147-149下轉163. doi: 復制

1.	Kamb A, Gruis NA, Weaver FT, et al. A cell cycle regulator potentially in genesis of many tumor types 〔Ｊ〕. Science, 1994; 264(2)∶436.
2.	Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
3.	Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
4.	Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
5.	Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
6.	Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
7.	沈凱，唐孝達，楊宇如. D類細胞周期素與癌基因和抑癌基因〔Ｊ〕. 中華醫學遺傳學雜志， 1996； 13(6)∶361.
8.	Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
9.	Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
10.	Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.

1. Kamb A, Gruis NA, Weaver FT, et al. A cell cycle regulator potentially in genesis of many tumor types 〔Ｊ〕. Science, 1994; 264(2)∶436.
2. Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
3. Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
4. Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
5. Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
6. Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
7. 沈凱，唐孝達，楊宇如. D類細胞周期素與癌基因和抑癌基因〔Ｊ〕. 中華醫學遺傳學雜志， 1996； 13(6)∶361.
8. Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
9. Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
10. Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.

《中國普外基礎與臨床雜志》

CyclinD1基因擴增及其蛋白表達在乳腺癌中的意義

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《中國普外基礎與臨床雜志》

CyclinD1基因擴增及其蛋白表達在乳腺癌中的意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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